How can biomolecules improve mucoadhesion of oral insulin? A comprehensive insight using ex-vivo, in silico, and in vivo models
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/97933 |
Resumo: | Funding: Supported in part by UID/DTP/04138/2019 from FCT, Portugal and DREAMS (ULHT). SEM analysis was funded by FCT/MCTES for the financial support to CESAM (UIDP/50017/2020+UIDB/50017/2020), through national funds. |
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How can biomolecules improve mucoadhesion of oral insulin? A comprehensive insight using ex-vivo, in silico, and in vivo modelsDiabetes mellitusInsulinMarine-derived biomoleculesMucoadhesionNanoparticleOral deliveryBiochemistryMolecular BiologySDG 3 - Good Health and Well-beingSDG 14 - Life Below WaterFunding: Supported in part by UID/DTP/04138/2019 from FCT, Portugal and DREAMS (ULHT). SEM analysis was funded by FCT/MCTES for the financial support to CESAM (UIDP/50017/2020+UIDB/50017/2020), through national funds.Currently, insulin can only be administered through the subcutaneous route. Due to the flaws associated with this route, it is of interest to orally deliver this drug. However, insulin delivered orally has several barriers to overcome as it is degraded by the stomach’s low pH, enzymatic content, and poor absorption in the gastrointestinal tract. Polymers with marine source like chitosan are commonly used in nanotechnology and drug delivery due to their biocompatibility and special features. This work focuses on the preparation and characterization of mucoadhesive insulin-loaded polymeric nanoparticles. Results showed a suitable mean size for oral administration (<600 nm by dynamic laser scattering), spherical shape, encapsulation efficiency (59.8%), and high recovery yield (80.6%). Circular dichroism spectroscopy demonstrated that protein retained its secondary structure after encapsulation. Moreover, the mucoadhesive potential of the nanoparticles was assessed in silico and the results, corroborated with ex-vivo experiments, showed that using chitosan strongly increases mucoadhesion. Besides, in vitro and in vivo safety assessment of the final formulation were performed, showing no toxicity. Lastly, the insulin-loaded nanoparticles were effective in reducing diabetic rats’ glycemia. Overall, the coating of insulin-loaded nanoparticles with chitosan represents a potentially safe and promising approach to protect insulin and enhance peroral delivery.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)Centro de Estudos de Doenças Crónicas (CEDOC)DF – Departamento de FísicaRUNAmaral, MarianaMartins, Ana SofiaCatarino, JoséFaísca, PedroKumar, PradeepPinto, João F.Pinto, RuiCorreia, IsabelAscensão, LiaAfonso, Ricardo A.Gaspar, M. ManuelaCharmier, Adília J.Figueiredo, Isabel VitóriaReis, Catarina Pinto2020-05-18T22:32:56Z2020-052020-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/97933eng2218-273XPURE: 18208984https://doi.org/10.3390/biom10050675info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:44:48Zoai:run.unl.pt:10362/97933Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:38:52.348472Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
How can biomolecules improve mucoadhesion of oral insulin? A comprehensive insight using ex-vivo, in silico, and in vivo models |
| title |
How can biomolecules improve mucoadhesion of oral insulin? A comprehensive insight using ex-vivo, in silico, and in vivo models |
| spellingShingle |
How can biomolecules improve mucoadhesion of oral insulin? A comprehensive insight using ex-vivo, in silico, and in vivo models Amaral, Mariana Diabetes mellitus Insulin Marine-derived biomolecules Mucoadhesion Nanoparticle Oral delivery Biochemistry Molecular Biology SDG 3 - Good Health and Well-being SDG 14 - Life Below Water |
| title_short |
How can biomolecules improve mucoadhesion of oral insulin? A comprehensive insight using ex-vivo, in silico, and in vivo models |
| title_full |
How can biomolecules improve mucoadhesion of oral insulin? A comprehensive insight using ex-vivo, in silico, and in vivo models |
| title_fullStr |
How can biomolecules improve mucoadhesion of oral insulin? A comprehensive insight using ex-vivo, in silico, and in vivo models |
| title_full_unstemmed |
How can biomolecules improve mucoadhesion of oral insulin? A comprehensive insight using ex-vivo, in silico, and in vivo models |
| title_sort |
How can biomolecules improve mucoadhesion of oral insulin? A comprehensive insight using ex-vivo, in silico, and in vivo models |
| author |
Amaral, Mariana |
| author_facet |
Amaral, Mariana Martins, Ana Sofia Catarino, José Faísca, Pedro Kumar, Pradeep Pinto, João F. Pinto, Rui Correia, Isabel Ascensão, Lia Afonso, Ricardo A. Gaspar, M. Manuela Charmier, Adília J. Figueiredo, Isabel Vitória Reis, Catarina Pinto |
| author_role |
author |
| author2 |
Martins, Ana Sofia Catarino, José Faísca, Pedro Kumar, Pradeep Pinto, João F. Pinto, Rui Correia, Isabel Ascensão, Lia Afonso, Ricardo A. Gaspar, M. Manuela Charmier, Adília J. Figueiredo, Isabel Vitória Reis, Catarina Pinto |
| author2_role |
author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) Centro de Estudos de Doenças Crónicas (CEDOC) DF – Departamento de Física RUN |
| dc.contributor.author.fl_str_mv |
Amaral, Mariana Martins, Ana Sofia Catarino, José Faísca, Pedro Kumar, Pradeep Pinto, João F. Pinto, Rui Correia, Isabel Ascensão, Lia Afonso, Ricardo A. Gaspar, M. Manuela Charmier, Adília J. Figueiredo, Isabel Vitória Reis, Catarina Pinto |
| dc.subject.por.fl_str_mv |
Diabetes mellitus Insulin Marine-derived biomolecules Mucoadhesion Nanoparticle Oral delivery Biochemistry Molecular Biology SDG 3 - Good Health and Well-being SDG 14 - Life Below Water |
| topic |
Diabetes mellitus Insulin Marine-derived biomolecules Mucoadhesion Nanoparticle Oral delivery Biochemistry Molecular Biology SDG 3 - Good Health and Well-being SDG 14 - Life Below Water |
| description |
Funding: Supported in part by UID/DTP/04138/2019 from FCT, Portugal and DREAMS (ULHT). SEM analysis was funded by FCT/MCTES for the financial support to CESAM (UIDP/50017/2020+UIDB/50017/2020), through national funds. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020-05-18T22:32:56Z 2020-05 2020-05-01T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/97933 |
| url |
http://hdl.handle.net/10362/97933 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
2218-273X PURE: 18208984 https://doi.org/10.3390/biom10050675 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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