Isolation of a Xanthobacter sp. degrading dichloromethane and characterization of the gene involved in the degradation
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/3262 |
Resumo: | A bacterial strain able to degrade dichloromethane (DCM) as the sole carbon source was isolated from a wastewater treatment plant receiving domestic and pharmaceutical effluent. 16S rDNA studies revealed the strain to be a Xanthobacter sp. (strain TM1). The new isolated strain when grown aerobically on DCM showed Luong type growth kinetics, with lmax of 0.094 h-1 and Sm of 1,435 mg l-1. Strain TM1 was able to degrade other aromatic and aliphatic halogenated compounds, such as halobenzoates, 2-chloroethanol and dichloroethane. The gene for DCM dehalogenase, which is the key enzyme in DCM degradation, was amplified through PCR reactions. Strain TM1 contains type A DCM dehalogenase (dcmAa), while no product could be obtained for type B dehalogense (dcmAb). The sequence was compared against 12 dcmAa from other DCM degrading strains and 98% or 99% similarity was observed with all other previously isolated DCM dehalogenase genes. This is the first time a Xanthobacter sp. is reported to degrade DCM. |
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Isolation of a Xanthobacter sp. degrading dichloromethane and characterization of the gene involved in the degradationDechlorinationDichloromethaneDCM dehalogenaseHalogenated organicXanthobacterA bacterial strain able to degrade dichloromethane (DCM) as the sole carbon source was isolated from a wastewater treatment plant receiving domestic and pharmaceutical effluent. 16S rDNA studies revealed the strain to be a Xanthobacter sp. (strain TM1). The new isolated strain when grown aerobically on DCM showed Luong type growth kinetics, with lmax of 0.094 h-1 and Sm of 1,435 mg l-1. Strain TM1 was able to degrade other aromatic and aliphatic halogenated compounds, such as halobenzoates, 2-chloroethanol and dichloroethane. The gene for DCM dehalogenase, which is the key enzyme in DCM degradation, was amplified through PCR reactions. Strain TM1 contains type A DCM dehalogenase (dcmAa), while no product could be obtained for type B dehalogense (dcmAb). The sequence was compared against 12 dcmAa from other DCM degrading strains and 98% or 99% similarity was observed with all other previously isolated DCM dehalogenase genes. This is the first time a Xanthobacter sp. is reported to degrade DCM.Springer VerlagVeritati - Repositório Institucional da Universidade Católica PortuguesaEmanuelsson, Maria A. E.Osuna, M. BegoñaJorge, Ruben M. FerreiraCastro, Paula M. L.2010-11-02T14:39:45Z20092009-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/3262engEMANUELSSON, Maria A. E ...[et al.] - Isolation of a xanthobacter sp. degrading dichloromethane. Biodegradation. ISSN 1572-9729. Vol. 20, n.º 2 (2009), p. 235–244info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-12T17:09:55ZPortal AgregadorONG |
dc.title.none.fl_str_mv |
Isolation of a Xanthobacter sp. degrading dichloromethane and characterization of the gene involved in the degradation |
title |
Isolation of a Xanthobacter sp. degrading dichloromethane and characterization of the gene involved in the degradation |
spellingShingle |
Isolation of a Xanthobacter sp. degrading dichloromethane and characterization of the gene involved in the degradation Emanuelsson, Maria A. E. Dechlorination Dichloromethane DCM dehalogenase Halogenated organic Xanthobacter |
title_short |
Isolation of a Xanthobacter sp. degrading dichloromethane and characterization of the gene involved in the degradation |
title_full |
Isolation of a Xanthobacter sp. degrading dichloromethane and characterization of the gene involved in the degradation |
title_fullStr |
Isolation of a Xanthobacter sp. degrading dichloromethane and characterization of the gene involved in the degradation |
title_full_unstemmed |
Isolation of a Xanthobacter sp. degrading dichloromethane and characterization of the gene involved in the degradation |
title_sort |
Isolation of a Xanthobacter sp. degrading dichloromethane and characterization of the gene involved in the degradation |
author |
Emanuelsson, Maria A. E. |
author_facet |
Emanuelsson, Maria A. E. Osuna, M. Begoña Jorge, Ruben M. Ferreira Castro, Paula M. L. |
author_role |
author |
author2 |
Osuna, M. Begoña Jorge, Ruben M. Ferreira Castro, Paula M. L. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Emanuelsson, Maria A. E. Osuna, M. Begoña Jorge, Ruben M. Ferreira Castro, Paula M. L. |
dc.subject.por.fl_str_mv |
Dechlorination Dichloromethane DCM dehalogenase Halogenated organic Xanthobacter |
topic |
Dechlorination Dichloromethane DCM dehalogenase Halogenated organic Xanthobacter |
description |
A bacterial strain able to degrade dichloromethane (DCM) as the sole carbon source was isolated from a wastewater treatment plant receiving domestic and pharmaceutical effluent. 16S rDNA studies revealed the strain to be a Xanthobacter sp. (strain TM1). The new isolated strain when grown aerobically on DCM showed Luong type growth kinetics, with lmax of 0.094 h-1 and Sm of 1,435 mg l-1. Strain TM1 was able to degrade other aromatic and aliphatic halogenated compounds, such as halobenzoates, 2-chloroethanol and dichloroethane. The gene for DCM dehalogenase, which is the key enzyme in DCM degradation, was amplified through PCR reactions. Strain TM1 contains type A DCM dehalogenase (dcmAa), while no product could be obtained for type B dehalogense (dcmAb). The sequence was compared against 12 dcmAa from other DCM degrading strains and 98% or 99% similarity was observed with all other previously isolated DCM dehalogenase genes. This is the first time a Xanthobacter sp. is reported to degrade DCM. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009 2009-01-01T00:00:00Z 2010-11-02T14:39:45Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/3262 |
url |
http://hdl.handle.net/10400.14/3262 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
EMANUELSSON, Maria A. E ...[et al.] - Isolation of a xanthobacter sp. degrading dichloromethane. Biodegradation. ISSN 1572-9729. Vol. 20, n.º 2 (2009), p. 235–244 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer Verlag |
publisher.none.fl_str_mv |
Springer Verlag |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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1777303117379928064 |