Validation of a Standard Luminescence Method for the Fast Determination of the Antimicrobial Activity of Nanoparticles in Escherichia coli

Detalhes bibliográficos
Autor(a) principal: Marcelo, Gonçalo A.
Data de Publicação: 2022
Outros Autores: Galhano, Joana, Duarte, Maria Paula, Capelo-Martínez, José Luis, Lodeiro, Carlos, Oliveira, Elisabete
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/143867
Resumo: Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
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spelling Validation of a Standard Luminescence Method for the Fast Determination of the Antimicrobial Activity of Nanoparticles in Escherichia coliantimicrobial activity assessmentE. coliluminescenceminimum inhibitory concentrationnanoparticlesChemical Engineering(all)Materials Science(all)Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.The use of nanoparticles in multiple industries has raised concerned voices about the assessment of their toxicity/antimicrobial activity and the development of standardized handling protocols. Issues emerge during the antimicrobial assaying of multiple cargo, colorimetric, colloidal nanoformulations, as standard protocols often rely on visual evaluations, or optical density (OD) measurements, leading to high variance inhibitory concentrations (MIC). Thus, a fast, luminescence-based assay for the effective assessment of the antimicrobial activity of nanoparticles is herein reported, using the bioluminescence of an in-house E. coli ATCC® 8739™ construct with the pMV306G13 + Lux plasmid (E. coli Lux). The new strain’s sensitivity to ofloxacin as a standard antibiotic was confirmed, and the methodology robustness verified against multiple nanoparticles and colorimetric drugs. The reduction of incubation from 24 to only 8 h, and the sole use of luminescence (LUX490) to accurately determine and distinguish MIC50 and MIC90, are two main advantages of the method. By discarding OD measurements, one can avoid turbidity and color interferences when calculating bacterial growth. This approach is an important tool that contributes to the standardization of methods, reducing samples’ background interference and focusing on luminescence as a direct probe for bacterial metabolic activity, growth and, most importantly, the correct assessment of nanomaterials’ antimicrobial activity.LAQV@REQUIMTEDQ - Departamento de QuímicaMEtRICS - Centro de Engenharia Mecânica e Sustentabilidade de RecursosRUNMarcelo, Gonçalo A.Galhano, JoanaDuarte, Maria PaulaCapelo-Martínez, José LuisLodeiro, CarlosOliveira, Elisabete2022-09-20T22:26:03Z2022-06-232022-06-23T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article22application/pdfhttp://hdl.handle.net/10362/143867eng2079-4991PURE: 46651221https://doi.org/10.3390/nano12132164info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:22:42Zoai:run.unl.pt:10362/143867Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:51:10.953187Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Validation of a Standard Luminescence Method for the Fast Determination of the Antimicrobial Activity of Nanoparticles in Escherichia coli
title Validation of a Standard Luminescence Method for the Fast Determination of the Antimicrobial Activity of Nanoparticles in Escherichia coli
spellingShingle Validation of a Standard Luminescence Method for the Fast Determination of the Antimicrobial Activity of Nanoparticles in Escherichia coli
Marcelo, Gonçalo A.
antimicrobial activity assessment
E. coli
luminescence
minimum inhibitory concentration
nanoparticles
Chemical Engineering(all)
Materials Science(all)
title_short Validation of a Standard Luminescence Method for the Fast Determination of the Antimicrobial Activity of Nanoparticles in Escherichia coli
title_full Validation of a Standard Luminescence Method for the Fast Determination of the Antimicrobial Activity of Nanoparticles in Escherichia coli
title_fullStr Validation of a Standard Luminescence Method for the Fast Determination of the Antimicrobial Activity of Nanoparticles in Escherichia coli
title_full_unstemmed Validation of a Standard Luminescence Method for the Fast Determination of the Antimicrobial Activity of Nanoparticles in Escherichia coli
title_sort Validation of a Standard Luminescence Method for the Fast Determination of the Antimicrobial Activity of Nanoparticles in Escherichia coli
author Marcelo, Gonçalo A.
author_facet Marcelo, Gonçalo A.
Galhano, Joana
Duarte, Maria Paula
Capelo-Martínez, José Luis
Lodeiro, Carlos
Oliveira, Elisabete
author_role author
author2 Galhano, Joana
Duarte, Maria Paula
Capelo-Martínez, José Luis
Lodeiro, Carlos
Oliveira, Elisabete
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv LAQV@REQUIMTE
DQ - Departamento de Química
MEtRICS - Centro de Engenharia Mecânica e Sustentabilidade de Recursos
RUN
dc.contributor.author.fl_str_mv Marcelo, Gonçalo A.
Galhano, Joana
Duarte, Maria Paula
Capelo-Martínez, José Luis
Lodeiro, Carlos
Oliveira, Elisabete
dc.subject.por.fl_str_mv antimicrobial activity assessment
E. coli
luminescence
minimum inhibitory concentration
nanoparticles
Chemical Engineering(all)
Materials Science(all)
topic antimicrobial activity assessment
E. coli
luminescence
minimum inhibitory concentration
nanoparticles
Chemical Engineering(all)
Materials Science(all)
description Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
publishDate 2022
dc.date.none.fl_str_mv 2022-09-20T22:26:03Z
2022-06-23
2022-06-23T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/143867
url http://hdl.handle.net/10362/143867
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2079-4991
PURE: 46651221
https://doi.org/10.3390/nano12132164
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 22
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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