Design of a custom RT-qPCR array for assignement of abiotic stress tolerance in traditional portuguese grapevine varieties

Detalhes bibliográficos
Autor(a) principal: Carvalho, Luísa C.
Data de Publicação: 2017
Outros Autores: Silva, Marilia, Coito, João L., Rocheta, Margarida P., Amâncio, Sara
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/14456
Resumo: Original Research
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spelling Design of a custom RT-qPCR array for assignement of abiotic stress tolerance in traditional portuguese grapevine varietiesabiotic stressacclimationcustom arraygrapevineqPCRstress tolerancetranscriptomicsOriginal ResearchWidespread agricultural losses attributed to drought, often combined with high temperatures, frequently occur in the field, namely in Mediterranean climate areas, where the existing scenarios for climate change indicate an increase in the frequency of heat waves and severe drought events in summer. Grapevine (Vitis vinifera L.) is the most cultivated fruit species in the world and the most valuable one and is a traditional Mediterranean species. Currently, viticulture must adjust to impending climate changes that are already pushing vine-growers toward the use of ancient and resilient varieties. Portugal is very rich in grapevine biodiversity, however, currently, 90% of the total producing area is planted with only 16 varieties. There is a pressing need to understand the existing genetic diversity and the physiological potential of the varieties/genotypes available to be able to respond to climate changes. With the above scenario in mind, an assembly of 65 differentially expresses genes (DEGs) previously identified as responsive to abiotic stresses in two well studied genotypes, ‘Touriga Nacional’ and ‘Trincadeira,’ was designed to scan the gene expression of leaf samples from 10 traditional Portuguese varieties growing in two regions with distinct environmental conditions. Forty-five of those DEGs proved to be associated to “abiotic stress” and were chosen to build a custom qPCR array to identify uncharacterized genotypes as sensitive or tolerant to abiotic stress. According to the experimental set-up behind the array design these DEGs can also be used as indicators of the main abiotic stress that the plant is subjected and responding to (drought, heat, or excess lightFrontiersRepositório da Universidade de LisboaCarvalho, Luísa C.Silva, MariliaCoito, João L.Rocheta, Margarida P.Amâncio, Sara2017-12-11T14:25:22Z2017-102017-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/14456engCarvalho LC, Silva M, Coito JL, Rocheta MP and Amâncio S (2017) Design of a Custom RT-qPCR Array for Assignment of Abiotic Stress Tolerance in Traditional Portuguese Grapevine Varieties. Front. Plant Sci. 8:1835.10.3389/fpls.2017.01835info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:44:30Zoai:www.repository.utl.pt:10400.5/14456Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:00:16.777648Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Design of a custom RT-qPCR array for assignement of abiotic stress tolerance in traditional portuguese grapevine varieties
title Design of a custom RT-qPCR array for assignement of abiotic stress tolerance in traditional portuguese grapevine varieties
spellingShingle Design of a custom RT-qPCR array for assignement of abiotic stress tolerance in traditional portuguese grapevine varieties
Carvalho, Luísa C.
abiotic stress
acclimation
custom array
grapevine
qPCR
stress tolerance
transcriptomics
title_short Design of a custom RT-qPCR array for assignement of abiotic stress tolerance in traditional portuguese grapevine varieties
title_full Design of a custom RT-qPCR array for assignement of abiotic stress tolerance in traditional portuguese grapevine varieties
title_fullStr Design of a custom RT-qPCR array for assignement of abiotic stress tolerance in traditional portuguese grapevine varieties
title_full_unstemmed Design of a custom RT-qPCR array for assignement of abiotic stress tolerance in traditional portuguese grapevine varieties
title_sort Design of a custom RT-qPCR array for assignement of abiotic stress tolerance in traditional portuguese grapevine varieties
author Carvalho, Luísa C.
author_facet Carvalho, Luísa C.
Silva, Marilia
Coito, João L.
Rocheta, Margarida P.
Amâncio, Sara
author_role author
author2 Silva, Marilia
Coito, João L.
Rocheta, Margarida P.
Amâncio, Sara
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Carvalho, Luísa C.
Silva, Marilia
Coito, João L.
Rocheta, Margarida P.
Amâncio, Sara
dc.subject.por.fl_str_mv abiotic stress
acclimation
custom array
grapevine
qPCR
stress tolerance
transcriptomics
topic abiotic stress
acclimation
custom array
grapevine
qPCR
stress tolerance
transcriptomics
description Original Research
publishDate 2017
dc.date.none.fl_str_mv 2017-12-11T14:25:22Z
2017-10
2017-10-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/14456
url http://hdl.handle.net/10400.5/14456
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Carvalho LC, Silva M, Coito JL, Rocheta MP and Amâncio S (2017) Design of a Custom RT-qPCR Array for Assignment of Abiotic Stress Tolerance in Traditional Portuguese Grapevine Varieties. Front. Plant Sci. 8:1835.
10.3389/fpls.2017.01835
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers
publisher.none.fl_str_mv Frontiers
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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