Cell-extrallular matrix interactions during early paraxial mesoderm development

Detalhes bibliográficos
Autor(a) principal: Lopes, Catarina Teixeira
Data de Publicação: 2008
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/1222
Resumo: Tese de mestrado, Biologia (Biologia Evolutiva e do Desenvolvimento), 2007, Universidade de Lisboa, Faculdade de Ciências
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spelling Cell-extrallular matrix interactions during early paraxial mesoderm developmentEmbriologiaBiologia evolutivaTeses de mestradoTese de mestrado, Biologia (Biologia Evolutiva e do Desenvolvimento), 2007, Universidade de Lisboa, Faculdade de CiênciasFibronectin is an abundant glycoprotein of the extracellular matrix, which has been shown to play a role in a variety of functions such as cell proliferation, survival, migration and differentiation. Fibronectin is assembled into a fibrillar matrix through a cell-mediated process involving their heterodimeric membrane receptors, integrins. Recently, the work of our group has evidenced the importance of fibronectin in somitogenesis and how the ectoderm and mesoderm collaborate in the construction of the fibronectin matrix of presomitic mesoderm (Rifes et al., 2007). In the present work, we further characterised the expression patterns of fibronectin (Fn1), integrin α5 (Itga5), the main sub-unit involved in fibronectin assembly, and integrin α4 (Itga4), during chick embryonic development. Fn1 transcripts are strongly expressed in most epithelia, mainly in the ectoderm in all stages studied. Itga5 is initially expressed in the primitive streak and node and it is later found in the ingressing mesoderm and many of its derivates, especially the heart and vascular primordia. Itga4 transcripts are also detected in the node and mesoderm, but later it is expressed in the dorsal neural tube and somite derivates. Finally, we also established an in vitro culture system of presomitic mesoderm cells by using different enzymes and substrata. Immunofluorescence labelling of fibronectin shows that isolated PSM cells in culture can assembly a fibronectin matrix, even when it was previously removed by dispase. In the tested culture conditions, these cells remained in a non-proliferative mesenchymal-like state, expressing very low levels of both N-cadherin and ß-catenin. These data suggest that fibronectin is not only an important component of the extracellular matrix in vivo and in vitro, but also that its presence in various tissues during the early stages of chick development is imperativeRodrigues, Maria GabrielaRepositório da Universidade de LisboaLopes, Catarina Teixeira2010-07-27T08:55:35Z20082008-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdftext/xmlhttp://hdl.handle.net/10451/1222porhttp://catalogo.ul.pt/F/?func=item-global&doc_library=ULB01&type=03&doc_number=000559481info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T15:40:34Zoai:repositorio.ul.pt:10451/1222Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:27:52.949135Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Cell-extrallular matrix interactions during early paraxial mesoderm development
title Cell-extrallular matrix interactions during early paraxial mesoderm development
spellingShingle Cell-extrallular matrix interactions during early paraxial mesoderm development
Lopes, Catarina Teixeira
Embriologia
Biologia evolutiva
Teses de mestrado
title_short Cell-extrallular matrix interactions during early paraxial mesoderm development
title_full Cell-extrallular matrix interactions during early paraxial mesoderm development
title_fullStr Cell-extrallular matrix interactions during early paraxial mesoderm development
title_full_unstemmed Cell-extrallular matrix interactions during early paraxial mesoderm development
title_sort Cell-extrallular matrix interactions during early paraxial mesoderm development
author Lopes, Catarina Teixeira
author_facet Lopes, Catarina Teixeira
author_role author
dc.contributor.none.fl_str_mv Rodrigues, Maria Gabriela
Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Lopes, Catarina Teixeira
dc.subject.por.fl_str_mv Embriologia
Biologia evolutiva
Teses de mestrado
topic Embriologia
Biologia evolutiva
Teses de mestrado
description Tese de mestrado, Biologia (Biologia Evolutiva e do Desenvolvimento), 2007, Universidade de Lisboa, Faculdade de Ciências
publishDate 2008
dc.date.none.fl_str_mv 2008
2008-01-01T00:00:00Z
2010-07-27T08:55:35Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/1222
url http://hdl.handle.net/10451/1222
dc.language.iso.fl_str_mv por
language por
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
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