Chitosan-olive oil microparticles for phenylethyl isothiocyanate delivery: optimal formulation

Detalhes bibliográficos
Autor(a) principal: Coscueta, Ezequiel R.
Data de Publicação: 2021
Outros Autores: Sousa, Ana Sofia, Reis, Celso A., Pintado, Manuela
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.14/33166
Resumo: Phenylethyl isothiocyanate (PEITC), a chemopreventive compound, is highly reactive due to its considerably electrophilic nature. Furthermore, it is hydrophobic and has low stability, bioavailability and bioaccessibility. This restricts its use in biomedical and nutraceutical or food applications. Thus, the encapsulation of this agent has the function of overcoming these limitations, promoting its solubility in water, and stabilizing it, preserving its bioactivity. So, polymeric microparticles were developed using chitosan-olive oil-PEITC systems. For this, an optimisation process (factors: olive oil: chitosan ratio and PEITC: chitosan ratio) was implemented through a 3-level factorial experimental design. The responses were: the particle size, zeta-potential, polydisperse index, and entrapment efficiency. The optimal formulation was further characterised by FTIR and biocompatibility in Caco-2 cells. Optimal conditions were olive oil: chitosan and PEITC: chitosan ratios of 1.46 and 0.25, respectively. These microparticles had a size of 629 nm, a zeta-potential of 32.3 mV, a polydispersity index of 0.329, and entrapment efficiency of 98.49%. We found that the inclusion process affected the optical behaviour of the PEITC, as well as the microparticles themselves and their interaction with the medium. Furthermore, the microparticles did not show cytotoxicity within the therapeutic values of PEITC. Thus, PEITC was microencapsulated with characteristics suitable for potential biomedical, nutraceutical and food applications.
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spelling Chitosan-olive oil microparticles for phenylethyl isothiocyanate delivery: optimal formulationPhenylethyl isothiocyanate (PEITC), a chemopreventive compound, is highly reactive due to its considerably electrophilic nature. Furthermore, it is hydrophobic and has low stability, bioavailability and bioaccessibility. This restricts its use in biomedical and nutraceutical or food applications. Thus, the encapsulation of this agent has the function of overcoming these limitations, promoting its solubility in water, and stabilizing it, preserving its bioactivity. So, polymeric microparticles were developed using chitosan-olive oil-PEITC systems. For this, an optimisation process (factors: olive oil: chitosan ratio and PEITC: chitosan ratio) was implemented through a 3-level factorial experimental design. The responses were: the particle size, zeta-potential, polydisperse index, and entrapment efficiency. The optimal formulation was further characterised by FTIR and biocompatibility in Caco-2 cells. Optimal conditions were olive oil: chitosan and PEITC: chitosan ratios of 1.46 and 0.25, respectively. These microparticles had a size of 629 nm, a zeta-potential of 32.3 mV, a polydispersity index of 0.329, and entrapment efficiency of 98.49%. We found that the inclusion process affected the optical behaviour of the PEITC, as well as the microparticles themselves and their interaction with the medium. Furthermore, the microparticles did not show cytotoxicity within the therapeutic values of PEITC. Thus, PEITC was microencapsulated with characteristics suitable for potential biomedical, nutraceutical and food applications.Veritati - Repositório Institucional da Universidade Católica PortuguesaCoscueta, Ezequiel R.Sousa, Ana SofiaReis, Celso A.Pintado, Manuela2021-05-23T15:38:35Z2021-052021-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/33166eng1932-620310.1371/journal.pone.024825785105271765PMC810172833956792000664611400009info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-12-19T01:37:40Zoai:repositorio.ucp.pt:10400.14/33166Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:26:55.110768Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Chitosan-olive oil microparticles for phenylethyl isothiocyanate delivery: optimal formulation
title Chitosan-olive oil microparticles for phenylethyl isothiocyanate delivery: optimal formulation
spellingShingle Chitosan-olive oil microparticles for phenylethyl isothiocyanate delivery: optimal formulation
Coscueta, Ezequiel R.
title_short Chitosan-olive oil microparticles for phenylethyl isothiocyanate delivery: optimal formulation
title_full Chitosan-olive oil microparticles for phenylethyl isothiocyanate delivery: optimal formulation
title_fullStr Chitosan-olive oil microparticles for phenylethyl isothiocyanate delivery: optimal formulation
title_full_unstemmed Chitosan-olive oil microparticles for phenylethyl isothiocyanate delivery: optimal formulation
title_sort Chitosan-olive oil microparticles for phenylethyl isothiocyanate delivery: optimal formulation
author Coscueta, Ezequiel R.
author_facet Coscueta, Ezequiel R.
Sousa, Ana Sofia
Reis, Celso A.
Pintado, Manuela
author_role author
author2 Sousa, Ana Sofia
Reis, Celso A.
Pintado, Manuela
author2_role author
author
author
dc.contributor.none.fl_str_mv Veritati - Repositório Institucional da Universidade Católica Portuguesa
dc.contributor.author.fl_str_mv Coscueta, Ezequiel R.
Sousa, Ana Sofia
Reis, Celso A.
Pintado, Manuela
description Phenylethyl isothiocyanate (PEITC), a chemopreventive compound, is highly reactive due to its considerably electrophilic nature. Furthermore, it is hydrophobic and has low stability, bioavailability and bioaccessibility. This restricts its use in biomedical and nutraceutical or food applications. Thus, the encapsulation of this agent has the function of overcoming these limitations, promoting its solubility in water, and stabilizing it, preserving its bioactivity. So, polymeric microparticles were developed using chitosan-olive oil-PEITC systems. For this, an optimisation process (factors: olive oil: chitosan ratio and PEITC: chitosan ratio) was implemented through a 3-level factorial experimental design. The responses were: the particle size, zeta-potential, polydisperse index, and entrapment efficiency. The optimal formulation was further characterised by FTIR and biocompatibility in Caco-2 cells. Optimal conditions were olive oil: chitosan and PEITC: chitosan ratios of 1.46 and 0.25, respectively. These microparticles had a size of 629 nm, a zeta-potential of 32.3 mV, a polydispersity index of 0.329, and entrapment efficiency of 98.49%. We found that the inclusion process affected the optical behaviour of the PEITC, as well as the microparticles themselves and their interaction with the medium. Furthermore, the microparticles did not show cytotoxicity within the therapeutic values of PEITC. Thus, PEITC was microencapsulated with characteristics suitable for potential biomedical, nutraceutical and food applications.
publishDate 2021
dc.date.none.fl_str_mv 2021-05-23T15:38:35Z
2021-05
2021-05-01T00:00:00Z
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.14/33166
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dc.language.iso.fl_str_mv eng
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dc.relation.none.fl_str_mv 1932-6203
10.1371/journal.pone.0248257
85105271765
PMC8101728
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