Effects of high CO2 and hypoxia on the embryonic development of European cuttlefish (Sepia officinalis)

Detalhes bibliográficos
Autor(a) principal: Amaduzzi, Angelica
Data de Publicação: 2022
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.1/19330
Resumo: The increasing atmospheric CO2 concentrations due to anthropic influence is causing the acidification of the oceans, which often co-occurs with hypoxia events. These are becoming a growing concern for marine ecosystem and for aquaculture due to the potential consequences on early life stages of marine animals and in particular for invertebrates, who form their internal skeletons out of calcium carbonate. The European cuttlefish (Sepia officinalis) is an excellent model to assess the effects of climate change, since it possesses a calcium carbonate structure from early development that grows very fast with a short life cycle. The present thesis aims to determine the effects of two environmental stressors, high CO2 environment and hypoxia events, in European cuttlefish embryogenesis, hatching rates and physiological characterisation through metabolic rates. Two separate experiments were performed. During the first, the cuttlefish eggs were kept in an indoor semi-open system with temperature maintained stable at 20°C and exposed to an acute event of hypoxia during the embryonic development (either day 10 or day 20). In the second experiment, cuttlefish eggs were kept in an outdoor open seawater system and exposed to high CO2 or to high CO2 plus an acute hypoxia event (either day 10 or day 20). The data obtained from the first experiment suggested that the embryos, kept at a temperature of 20°C can manage an acute hypoxia event without substantial consequences on their development. In the second outdoor experiment a much lower hatching rate was recorded, compared to the first experiment. This might be reconnected to the higher water temperature. Based on these data, it can be hypothesised that temperature could be one of the main key factors for cuttlefish eggs survival. Even so, it is not clear which is the threshold temperature for cuttlefish during the embryonic development.
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spelling Effects of high CO2 and hypoxia on the embryonic development of European cuttlefish (Sepia officinalis)CO2CuttlefishEmbryogenesisHypoxiaMetabolismSepia officinalisThe increasing atmospheric CO2 concentrations due to anthropic influence is causing the acidification of the oceans, which often co-occurs with hypoxia events. These are becoming a growing concern for marine ecosystem and for aquaculture due to the potential consequences on early life stages of marine animals and in particular for invertebrates, who form their internal skeletons out of calcium carbonate. The European cuttlefish (Sepia officinalis) is an excellent model to assess the effects of climate change, since it possesses a calcium carbonate structure from early development that grows very fast with a short life cycle. The present thesis aims to determine the effects of two environmental stressors, high CO2 environment and hypoxia events, in European cuttlefish embryogenesis, hatching rates and physiological characterisation through metabolic rates. Two separate experiments were performed. During the first, the cuttlefish eggs were kept in an indoor semi-open system with temperature maintained stable at 20°C and exposed to an acute event of hypoxia during the embryonic development (either day 10 or day 20). In the second experiment, cuttlefish eggs were kept in an outdoor open seawater system and exposed to high CO2 or to high CO2 plus an acute hypoxia event (either day 10 or day 20). The data obtained from the first experiment suggested that the embryos, kept at a temperature of 20°C can manage an acute hypoxia event without substantial consequences on their development. In the second outdoor experiment a much lower hatching rate was recorded, compared to the first experiment. This might be reconnected to the higher water temperature. Based on these data, it can be hypothesised that temperature could be one of the main key factors for cuttlefish eggs survival. Even so, it is not clear which is the threshold temperature for cuttlefish during the embryonic development.Fuentes, JuanSykes, AntónioSapientiaAmaduzzi, Angelica2023-03-28T11:08:03Z2022-10-212022-10-21T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10400.1/19330enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-11-29T10:28:15Zoai:sapientia.ualg.pt:10400.1/19330Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-11-29T10:28:15Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Effects of high CO2 and hypoxia on the embryonic development of European cuttlefish (Sepia officinalis)
title Effects of high CO2 and hypoxia on the embryonic development of European cuttlefish (Sepia officinalis)
spellingShingle Effects of high CO2 and hypoxia on the embryonic development of European cuttlefish (Sepia officinalis)
Amaduzzi, Angelica
CO2
Cuttlefish
Embryogenesis
Hypoxia
Metabolism
Sepia officinalis
title_short Effects of high CO2 and hypoxia on the embryonic development of European cuttlefish (Sepia officinalis)
title_full Effects of high CO2 and hypoxia on the embryonic development of European cuttlefish (Sepia officinalis)
title_fullStr Effects of high CO2 and hypoxia on the embryonic development of European cuttlefish (Sepia officinalis)
title_full_unstemmed Effects of high CO2 and hypoxia on the embryonic development of European cuttlefish (Sepia officinalis)
title_sort Effects of high CO2 and hypoxia on the embryonic development of European cuttlefish (Sepia officinalis)
author Amaduzzi, Angelica
author_facet Amaduzzi, Angelica
author_role author
dc.contributor.none.fl_str_mv Fuentes, Juan
Sykes, António
Sapientia
dc.contributor.author.fl_str_mv Amaduzzi, Angelica
dc.subject.por.fl_str_mv CO2
Cuttlefish
Embryogenesis
Hypoxia
Metabolism
Sepia officinalis
topic CO2
Cuttlefish
Embryogenesis
Hypoxia
Metabolism
Sepia officinalis
description The increasing atmospheric CO2 concentrations due to anthropic influence is causing the acidification of the oceans, which often co-occurs with hypoxia events. These are becoming a growing concern for marine ecosystem and for aquaculture due to the potential consequences on early life stages of marine animals and in particular for invertebrates, who form their internal skeletons out of calcium carbonate. The European cuttlefish (Sepia officinalis) is an excellent model to assess the effects of climate change, since it possesses a calcium carbonate structure from early development that grows very fast with a short life cycle. The present thesis aims to determine the effects of two environmental stressors, high CO2 environment and hypoxia events, in European cuttlefish embryogenesis, hatching rates and physiological characterisation through metabolic rates. Two separate experiments were performed. During the first, the cuttlefish eggs were kept in an indoor semi-open system with temperature maintained stable at 20°C and exposed to an acute event of hypoxia during the embryonic development (either day 10 or day 20). In the second experiment, cuttlefish eggs were kept in an outdoor open seawater system and exposed to high CO2 or to high CO2 plus an acute hypoxia event (either day 10 or day 20). The data obtained from the first experiment suggested that the embryos, kept at a temperature of 20°C can manage an acute hypoxia event without substantial consequences on their development. In the second outdoor experiment a much lower hatching rate was recorded, compared to the first experiment. This might be reconnected to the higher water temperature. Based on these data, it can be hypothesised that temperature could be one of the main key factors for cuttlefish eggs survival. Even so, it is not clear which is the threshold temperature for cuttlefish during the embryonic development.
publishDate 2022
dc.date.none.fl_str_mv 2022-10-21
2022-10-21T00:00:00Z
2023-03-28T11:08:03Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.1/19330
url http://hdl.handle.net/10400.1/19330
dc.language.iso.fl_str_mv eng
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dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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