Interindividual heterogeneity affects the outcome of human cardiac tissue decellularization

Detalhes bibliográficos
Autor(a) principal: Tenreiro, Miguel F.
Data de Publicação: 2021
Outros Autores: Almeida, Henrique V., Calmeiro, Tomás, Fortunato, Elvira, Ferreira, Lino, Alves, Paula M., Serra, Margarida
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/128465
Resumo: Funding Information: The work here presented was funded by Fundação para a Ciência e Tecnologia (FCT) projects NETDIAMOND (SAICTPAC/0047/2015), financially supported by FEEI-Lisboa2020 and FCT/POCI-01-0145-FEDER-016385, and MetaCardio (PTDC/BTM-SAL/32566/2017); iNOVA4Health-UIDB/04462/2020 and UIDP/04462/2020, a program financially supported by FCT/Ministério da Ciência, Tecnologia e Ensino Superior, through national funds; and EU-funded projects BRAV3 (H2020, ID:874827), ERAatUC (ID: 669088) and Twinning RESETageing (ID: 952266). HVA was financed by FCT Grant SFRH/BPD/120595/2016.
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spelling Interindividual heterogeneity affects the outcome of human cardiac tissue decellularizationGeneralFunding Information: The work here presented was funded by Fundação para a Ciência e Tecnologia (FCT) projects NETDIAMOND (SAICTPAC/0047/2015), financially supported by FEEI-Lisboa2020 and FCT/POCI-01-0145-FEDER-016385, and MetaCardio (PTDC/BTM-SAL/32566/2017); iNOVA4Health-UIDB/04462/2020 and UIDP/04462/2020, a program financially supported by FCT/Ministério da Ciência, Tecnologia e Ensino Superior, through national funds; and EU-funded projects BRAV3 (H2020, ID:874827), ERAatUC (ID: 669088) and Twinning RESETageing (ID: 952266). HVA was financed by FCT Grant SFRH/BPD/120595/2016.The extracellular matrix (ECM) of engineered human cardiac tissues corresponds to simplistic biomaterials that allow tissue assembly, or animal derived off-the-shelf non-cardiac specific matrices. Decellularized ECM from human cardiac tissue could provide a means to improve the mimicry of engineered human cardiac tissues. Decellularization of cardiac tissue samples using immersion-based methods can produce acceptable cardiac ECM scaffolds; however, these protocols are mostly described for animal tissue preparations. We have tested four methods to decellularize human cardiac tissue and evaluated their efficiency in terms of cell removal and preservation of key ECM components, such as collagens and sulfated glycosaminoglycans. Extended exposure to decellularization agents, namely sodium dodecyl sulfate and Triton-X-100, was needed to significantly remove DNA content by approximately 93% in all human donors. However, the biochemical composition of decellularized tissue is affected, and the preservation of ECM architecture is donor dependent. Our results indicate that standardization of decellularization protocols for human tissue is likely unfeasible, and a compromise between cell removal and ECM preservation must be established in accordance with the scaffold’s intended application. Notwithstanding, decellularized human cardiac ECM supported human induced pluripotent-derived cardiomyocyte (hiPSC-CM) attachment and retention for up to 2 weeks of culture, and promoted cell alignment and contraction, providing evidence it could be a valuable tool for cardiac tissue engineering.CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)DCM - Departamento de Ciência dos MateriaisProgramme in Translational Medicine (iNOVA4Health)Instituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNTenreiro, Miguel F.Almeida, Henrique V.Calmeiro, TomásFortunato, ElviraFerreira, LinoAlves, Paula M.Serra, Margarida2021-11-29T23:39:57Z2021-122021-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/128465eng2045-2322PURE: 35106474https://doi.org/10.1038/s41598-021-00226-5info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:08:03Zoai:run.unl.pt:10362/128465Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:46:18.813641Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Interindividual heterogeneity affects the outcome of human cardiac tissue decellularization
title Interindividual heterogeneity affects the outcome of human cardiac tissue decellularization
spellingShingle Interindividual heterogeneity affects the outcome of human cardiac tissue decellularization
Tenreiro, Miguel F.
General
title_short Interindividual heterogeneity affects the outcome of human cardiac tissue decellularization
title_full Interindividual heterogeneity affects the outcome of human cardiac tissue decellularization
title_fullStr Interindividual heterogeneity affects the outcome of human cardiac tissue decellularization
title_full_unstemmed Interindividual heterogeneity affects the outcome of human cardiac tissue decellularization
title_sort Interindividual heterogeneity affects the outcome of human cardiac tissue decellularization
author Tenreiro, Miguel F.
author_facet Tenreiro, Miguel F.
Almeida, Henrique V.
Calmeiro, Tomás
Fortunato, Elvira
Ferreira, Lino
Alves, Paula M.
Serra, Margarida
author_role author
author2 Almeida, Henrique V.
Calmeiro, Tomás
Fortunato, Elvira
Ferreira, Lino
Alves, Paula M.
Serra, Margarida
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)
DCM - Departamento de Ciência dos Materiais
Programme in Translational Medicine (iNOVA4Health)
Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
RUN
dc.contributor.author.fl_str_mv Tenreiro, Miguel F.
Almeida, Henrique V.
Calmeiro, Tomás
Fortunato, Elvira
Ferreira, Lino
Alves, Paula M.
Serra, Margarida
dc.subject.por.fl_str_mv General
topic General
description Funding Information: The work here presented was funded by Fundação para a Ciência e Tecnologia (FCT) projects NETDIAMOND (SAICTPAC/0047/2015), financially supported by FEEI-Lisboa2020 and FCT/POCI-01-0145-FEDER-016385, and MetaCardio (PTDC/BTM-SAL/32566/2017); iNOVA4Health-UIDB/04462/2020 and UIDP/04462/2020, a program financially supported by FCT/Ministério da Ciência, Tecnologia e Ensino Superior, through national funds; and EU-funded projects BRAV3 (H2020, ID:874827), ERAatUC (ID: 669088) and Twinning RESETageing (ID: 952266). HVA was financed by FCT Grant SFRH/BPD/120595/2016.
publishDate 2021
dc.date.none.fl_str_mv 2021-11-29T23:39:57Z
2021-12
2021-12-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/128465
url http://hdl.handle.net/10362/128465
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2045-2322
PURE: 35106474
https://doi.org/10.1038/s41598-021-00226-5
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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