In vitro characterization of 3D printed scaffolds aimed at bone tissue regeneration
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.6/4705 |
Resumo: | The incidence of fractures and bone-related diseases like osteoporosis has been increasing due to aging of the world’s population. Up to now, grafts and titanium implants have been the principal therapeutic approaches used for bone repair/regeneration. However, these types of treatment have several shortcomings, like limited availability, risk of donor-to-recipient infection and tissue morbidity. To overcome these handicaps, new 3D templates, capable of replicating the features of the native tissue, are currently being developed by researchers from the area of tissue engineering. These 3D constructs are able to provide a temporary matrix on which host cells can adhere, proliferate and differentiate. Herein, 3D cylindrical scaffolds were designed to mimic the natural architecture of hollow bones, and to allow nutrient exchange and bone neovascularization. 3D scaffolds were produced with tricalcium phosphate (TCP)/alginic acid (AA) using a Fab@home 3D printer. Furthermore, graphene oxide (GO) was incorporated into the structure of some scaffolds to further enhance their mechanical properties. The results revealed that the scaffolds incorporating GO displayed greater porosity, without impairing their mechanical properties. These scaffolds also presented a controlled swelling profile, enhanced biomineralization capacity and were able to increase the Alkaline Phosphatase (ALP) activity. Such characteristics make TCP/AA scaffolds functionalized with GO promising 3D constructs for bone tissue engineering applications. |
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In vitro characterization of 3D printed scaffolds aimed at bone tissue regenerationBone tissue engineeringRapid prototyping3D printingCylindrical hybrid scaffoldsGraphene oxideThe incidence of fractures and bone-related diseases like osteoporosis has been increasing due to aging of the world’s population. Up to now, grafts and titanium implants have been the principal therapeutic approaches used for bone repair/regeneration. However, these types of treatment have several shortcomings, like limited availability, risk of donor-to-recipient infection and tissue morbidity. To overcome these handicaps, new 3D templates, capable of replicating the features of the native tissue, are currently being developed by researchers from the area of tissue engineering. These 3D constructs are able to provide a temporary matrix on which host cells can adhere, proliferate and differentiate. Herein, 3D cylindrical scaffolds were designed to mimic the natural architecture of hollow bones, and to allow nutrient exchange and bone neovascularization. 3D scaffolds were produced with tricalcium phosphate (TCP)/alginic acid (AA) using a Fab@home 3D printer. Furthermore, graphene oxide (GO) was incorporated into the structure of some scaffolds to further enhance their mechanical properties. The results revealed that the scaffolds incorporating GO displayed greater porosity, without impairing their mechanical properties. These scaffolds also presented a controlled swelling profile, enhanced biomineralization capacity and were able to increase the Alkaline Phosphatase (ALP) activity. Such characteristics make TCP/AA scaffolds functionalized with GO promising 3D constructs for bone tissue engineering applications.ElsevieruBibliorumBoga, João Miguel Carvalho FreireMiguel, Sónia P.Diogo, Duarte Miguel de MeloMendonça, AntónioLouro, RicardoCorreia, Ilídio Joaquim Sobreira2018-03-22T09:46:46Z2018-02-212018-02-21T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.6/4705engBoga, J.C., Miguel, S.P., de Melo-Diogo, D., Mendonça, A.G., Louro, R.O., Correia, I.J. (2018) "In vitro characterization of 3D printed scaffolds aimed at bone tissue regeneration", Colloids and Surfaces B: Biointerfaces, Vol. 165, pp.207-21810.1016/j.colsurfb.2018.02.038metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-12-15T09:42:00Zoai:ubibliorum.ubi.pt:10400.6/4705Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:45:45.358917Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
In vitro characterization of 3D printed scaffolds aimed at bone tissue regeneration |
title |
In vitro characterization of 3D printed scaffolds aimed at bone tissue regeneration |
spellingShingle |
In vitro characterization of 3D printed scaffolds aimed at bone tissue regeneration Boga, João Miguel Carvalho Freire Bone tissue engineering Rapid prototyping 3D printing Cylindrical hybrid scaffolds Graphene oxide |
title_short |
In vitro characterization of 3D printed scaffolds aimed at bone tissue regeneration |
title_full |
In vitro characterization of 3D printed scaffolds aimed at bone tissue regeneration |
title_fullStr |
In vitro characterization of 3D printed scaffolds aimed at bone tissue regeneration |
title_full_unstemmed |
In vitro characterization of 3D printed scaffolds aimed at bone tissue regeneration |
title_sort |
In vitro characterization of 3D printed scaffolds aimed at bone tissue regeneration |
author |
Boga, João Miguel Carvalho Freire |
author_facet |
Boga, João Miguel Carvalho Freire Miguel, Sónia P. Diogo, Duarte Miguel de Melo Mendonça, António Louro, Ricardo Correia, Ilídio Joaquim Sobreira |
author_role |
author |
author2 |
Miguel, Sónia P. Diogo, Duarte Miguel de Melo Mendonça, António Louro, Ricardo Correia, Ilídio Joaquim Sobreira |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
uBibliorum |
dc.contributor.author.fl_str_mv |
Boga, João Miguel Carvalho Freire Miguel, Sónia P. Diogo, Duarte Miguel de Melo Mendonça, António Louro, Ricardo Correia, Ilídio Joaquim Sobreira |
dc.subject.por.fl_str_mv |
Bone tissue engineering Rapid prototyping 3D printing Cylindrical hybrid scaffolds Graphene oxide |
topic |
Bone tissue engineering Rapid prototyping 3D printing Cylindrical hybrid scaffolds Graphene oxide |
description |
The incidence of fractures and bone-related diseases like osteoporosis has been increasing due to aging of the world’s population. Up to now, grafts and titanium implants have been the principal therapeutic approaches used for bone repair/regeneration. However, these types of treatment have several shortcomings, like limited availability, risk of donor-to-recipient infection and tissue morbidity. To overcome these handicaps, new 3D templates, capable of replicating the features of the native tissue, are currently being developed by researchers from the area of tissue engineering. These 3D constructs are able to provide a temporary matrix on which host cells can adhere, proliferate and differentiate. Herein, 3D cylindrical scaffolds were designed to mimic the natural architecture of hollow bones, and to allow nutrient exchange and bone neovascularization. 3D scaffolds were produced with tricalcium phosphate (TCP)/alginic acid (AA) using a Fab@home 3D printer. Furthermore, graphene oxide (GO) was incorporated into the structure of some scaffolds to further enhance their mechanical properties. The results revealed that the scaffolds incorporating GO displayed greater porosity, without impairing their mechanical properties. These scaffolds also presented a controlled swelling profile, enhanced biomineralization capacity and were able to increase the Alkaline Phosphatase (ALP) activity. Such characteristics make TCP/AA scaffolds functionalized with GO promising 3D constructs for bone tissue engineering applications. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-03-22T09:46:46Z 2018-02-21 2018-02-21T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.6/4705 |
url |
http://hdl.handle.net/10400.6/4705 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Boga, J.C., Miguel, S.P., de Melo-Diogo, D., Mendonça, A.G., Louro, R.O., Correia, I.J. (2018) "In vitro characterization of 3D printed scaffolds aimed at bone tissue regeneration", Colloids and Surfaces B: Biointerfaces, Vol. 165, pp.207-218 10.1016/j.colsurfb.2018.02.038 |
dc.rights.driver.fl_str_mv |
metadata only access info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
metadata only access |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799136354576105472 |