Ammonium is a key determinant on the dietary restriction of yeast chronological aging in culture medium
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/32710 |
Resumo: | New evidences have recently emerged from studies in yeast and in higher eukaryotes showing the importance of nutrient balance in dietary regimes and its effects on longevity regulation.We have previously shown that manipulation ofammoniumconcentration in the culture and/or aging medium can drastically affect chronological lifespan (CLS)of Saccharomyces cerevisiae, especially in amino acid restricted cells. Here we describe that the CLS shortening under amino acid restriction can be completely reverted by removing ammonium from the culture medium. Furthermore, the absence of ammonium, and of any rich nitrogen source, was so effective in extending CLS that no beneficial effect could be observed by further imposing calorie restriction conditions. When present in the culture medium,ammoniumimpaired the consumption of theauxotrophy-complementing amino acidsand caused in an improper cell cycle arrest of the culture.TOR1deletion reverted ammonium effects both in amino acid restricted and non-restricted cultures, whereas, Ras2p and Sch9p seem to have only a milder effect in the mediation ofammonium toxicity under amino acid restriction and no effect on non-restricted cultures.Our studies highlight ammonium as a key effector in the nutritional equilibrium between rich and essential nitrogen sources and glucose required for longevity promotion. |
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Ammonium is a key determinant on the dietary restriction of yeast chronological aging in culture mediumAmmoniumlifespandietary restrictionamino acid restrictionyeastScience & TechnologyNew evidences have recently emerged from studies in yeast and in higher eukaryotes showing the importance of nutrient balance in dietary regimes and its effects on longevity regulation.We have previously shown that manipulation ofammoniumconcentration in the culture and/or aging medium can drastically affect chronological lifespan (CLS)of Saccharomyces cerevisiae, especially in amino acid restricted cells. Here we describe that the CLS shortening under amino acid restriction can be completely reverted by removing ammonium from the culture medium. Furthermore, the absence of ammonium, and of any rich nitrogen source, was so effective in extending CLS that no beneficial effect could be observed by further imposing calorie restriction conditions. When present in the culture medium,ammoniumimpaired the consumption of theauxotrophy-complementing amino acidsand caused in an improper cell cycle arrest of the culture.TOR1deletion reverted ammonium effects both in amino acid restricted and non-restricted cultures, whereas, Ras2p and Sch9p seem to have only a milder effect in the mediation ofammonium toxicity under amino acid restriction and no effect on non-restricted cultures.Our studies highlight ammonium as a key effector in the nutritional equilibrium between rich and essential nitrogen sources and glucose required for longevity promotion.Julia Santos holds a Post-Doc fellowship (UMINHO/ BPD / 39/ 2013) funded by QREN-FEDER.Impact JournalsUniversidade do MinhoSantos, JúliaCorreia, Fernanda LeitãoSousa, Maria JoãoLeão, Cecília20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/32710engSantos, J., Leitão-Correia, F., Sousa, M. J., & Leão, C. (2015). Ammonium is a key determinant on the dietary restriction of yeast chronological aging in culture medium. Oncotarget, 6(9), 6511-6523.1949-255310.18632/oncotarget.298925576917http://www.impactjournals.com/oncotarget/index.php?journal=oncotarget&page=article&op=view&path%5B%5D=2989&path%5B%5D=5700info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:08:12Zoai:repositorium.sdum.uminho.pt:1822/32710Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:59:24.990620Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Ammonium is a key determinant on the dietary restriction of yeast chronological aging in culture medium |
title |
Ammonium is a key determinant on the dietary restriction of yeast chronological aging in culture medium |
spellingShingle |
Ammonium is a key determinant on the dietary restriction of yeast chronological aging in culture medium Santos, Júlia Ammonium lifespan dietary restriction amino acid restriction yeast Science & Technology |
title_short |
Ammonium is a key determinant on the dietary restriction of yeast chronological aging in culture medium |
title_full |
Ammonium is a key determinant on the dietary restriction of yeast chronological aging in culture medium |
title_fullStr |
Ammonium is a key determinant on the dietary restriction of yeast chronological aging in culture medium |
title_full_unstemmed |
Ammonium is a key determinant on the dietary restriction of yeast chronological aging in culture medium |
title_sort |
Ammonium is a key determinant on the dietary restriction of yeast chronological aging in culture medium |
author |
Santos, Júlia |
author_facet |
Santos, Júlia Correia, Fernanda Leitão Sousa, Maria João Leão, Cecília |
author_role |
author |
author2 |
Correia, Fernanda Leitão Sousa, Maria João Leão, Cecília |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Santos, Júlia Correia, Fernanda Leitão Sousa, Maria João Leão, Cecília |
dc.subject.por.fl_str_mv |
Ammonium lifespan dietary restriction amino acid restriction yeast Science & Technology |
topic |
Ammonium lifespan dietary restriction amino acid restriction yeast Science & Technology |
description |
New evidences have recently emerged from studies in yeast and in higher eukaryotes showing the importance of nutrient balance in dietary regimes and its effects on longevity regulation.We have previously shown that manipulation ofammoniumconcentration in the culture and/or aging medium can drastically affect chronological lifespan (CLS)of Saccharomyces cerevisiae, especially in amino acid restricted cells. Here we describe that the CLS shortening under amino acid restriction can be completely reverted by removing ammonium from the culture medium. Furthermore, the absence of ammonium, and of any rich nitrogen source, was so effective in extending CLS that no beneficial effect could be observed by further imposing calorie restriction conditions. When present in the culture medium,ammoniumimpaired the consumption of theauxotrophy-complementing amino acidsand caused in an improper cell cycle arrest of the culture.TOR1deletion reverted ammonium effects both in amino acid restricted and non-restricted cultures, whereas, Ras2p and Sch9p seem to have only a milder effect in the mediation ofammonium toxicity under amino acid restriction and no effect on non-restricted cultures.Our studies highlight ammonium as a key effector in the nutritional equilibrium between rich and essential nitrogen sources and glucose required for longevity promotion. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015 2015-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/32710 |
url |
http://hdl.handle.net/1822/32710 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Santos, J., Leitão-Correia, F., Sousa, M. J., & Leão, C. (2015). Ammonium is a key determinant on the dietary restriction of yeast chronological aging in culture medium. Oncotarget, 6(9), 6511-6523. 1949-2553 10.18632/oncotarget.2989 25576917 http://www.impactjournals.com/oncotarget/index.php?journal=oncotarget&page=article&op=view&path%5B%5D=2989&path%5B%5D=5700 |
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info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Impact Journals |
publisher.none.fl_str_mv |
Impact Journals |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132386156347392 |