A One Health Approach Molecular Analysis of Staphylococcus aureus Reveals Distinct Lineages in Isolates from Miranda Donkeys (Equus asinus) and Their Handlers

Detalhes bibliográficos
Autor(a) principal: Silva, Vanessa
Data de Publicação: 2022
Outros Autores: Alfarela, Cláudia, Caniça, Manuela, Manageiro, Vera, Nóvoa, Miguel, Leiva, Belen, Kress, Maria, Capelo, José Luís, Poeta, Patrícia, Igrejas, Gilberto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/151036
Resumo: This work was funded by the R&D Project CAREBIO2: Comparative assessment of antimicrobial resistance in environmental biofilms through proteomics—towards innovative thera-nostic biomarkers, with reference NORTE-01-0145-FEDER-030101, financed by the European Regional Development Fund (ERDF) through the Northern Regional Operational Program (NORTE 2020) and the Foundation for Science and Technology (FCT). projects UIDB/CVT/00772/2020 and LA/P/0059/2020 funded by the Portuguese Foundation for Science and Technology (FCT). Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
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spelling A One Health Approach Molecular Analysis of Staphylococcus aureus Reveals Distinct Lineages in Isolates from Miranda Donkeys (Equus asinus) and Their HandlersAnimal-to-human transmissionCoNSDonkeysStaphylococcus aureusMicrobiologyBiochemistryPharmacology, Toxicology and Pharmaceutics(all)Microbiology (medical)Infectious DiseasesPharmacology (medical)SDG 3 - Good Health and Well-beingThis work was funded by the R&D Project CAREBIO2: Comparative assessment of antimicrobial resistance in environmental biofilms through proteomics—towards innovative thera-nostic biomarkers, with reference NORTE-01-0145-FEDER-030101, financed by the European Regional Development Fund (ERDF) through the Northern Regional Operational Program (NORTE 2020) and the Foundation for Science and Technology (FCT). projects UIDB/CVT/00772/2020 and LA/P/0059/2020 funded by the Portuguese Foundation for Science and Technology (FCT). Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.Donkeys (Equus asinus) are in decline in Europe. Occupational exposure to farm animals has been associated with increased staphylococci carriage. We aimed to isolate S. aureus and coagulase-negative staphylococci (CoNS) from donkeys and handlers and characterize the antimicro-bial resistance profiles and genetic lineages of S. aureus strains. Oral and nasal swab samples were collected from 49 Miranda donkeys and 23 handlers from 15 different farms. Staphylococci species were identified by MALDI-TOF MS. The presence of antimicrobial resistance genes and virulence factors was investigated by PCR. Molecular typing was performed in S. aureus isolates. From the 49 donkey samples, 4 S. aureus (8.2%) and 21 CoNS (42.9%) were isolated. Ten handlers (43.5%) were carriers of S. aureus and 4 (17.4%) carried CoNS. The CoNS isolates showed resistance to several classes of antimicrobials encoded by the mecA, aph (3′)-IIIa, ant (4′)-Ia, tetM, tetK, lnuA, ermB, ermC, dfrA and dfrG genes. S. aureus isolates were resistant to penicillin, aminoglicosides and tetracycline harboring the blaZ, aph (3′)-IIIa, tetL, tetM and tetK genes. All S. aureus isolates from donkeys belonged to ST49 and spa-type t208 while the strains isolated from the handlers were ascribed to 3 STs and 7 spa-types. However, human isolates were from different STs than the donkey isolates. Donkeys are mainly colonized by methicillin-resistant S. sciuri. S. aureus transmission between donkeys and their handlers appears not to have occurred since the isolates belonged to different genetic lineages.LAQV@REQUIMTEDQ - Departamento de QuímicaRUNSilva, VanessaAlfarela, CláudiaCaniça, ManuelaManageiro, VeraNóvoa, MiguelLeiva, BelenKress, MariaCapelo, José LuísPoeta, PatríciaIgrejas, Gilberto2023-03-21T22:29:14Z2022-03-102022-03-10T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11application/pdfhttp://hdl.handle.net/10362/151036engSilva, V., Alfarela, C., Caniça, M., Manageiro, V., Nóvoa, M., Leiva, B., Kress, M., Capelo, J. L., Poeta, P., & Igrejas, G. (2022). A One Health Approach Molecular Analysis of Staphylococcus aureus Reveals Distinct Lineages in Isolates from Miranda Donkeys (Equus asinus) and Their Handlers. Antibiotics, 11(3), [374]. https://doi.org/10.3390/antibiotics110303742079-6382PURE: 56530058https://doi.org/10.3390/antibiotics11030374info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-22T18:10:26Zoai:run.unl.pt:10362/151036Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-22T18:10:26Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A One Health Approach Molecular Analysis of Staphylococcus aureus Reveals Distinct Lineages in Isolates from Miranda Donkeys (Equus asinus) and Their Handlers
title A One Health Approach Molecular Analysis of Staphylococcus aureus Reveals Distinct Lineages in Isolates from Miranda Donkeys (Equus asinus) and Their Handlers
spellingShingle A One Health Approach Molecular Analysis of Staphylococcus aureus Reveals Distinct Lineages in Isolates from Miranda Donkeys (Equus asinus) and Their Handlers
Silva, Vanessa
Animal-to-human transmission
CoNS
Donkeys
Staphylococcus aureus
Microbiology
Biochemistry
Pharmacology, Toxicology and Pharmaceutics(all)
Microbiology (medical)
Infectious Diseases
Pharmacology (medical)
SDG 3 - Good Health and Well-being
title_short A One Health Approach Molecular Analysis of Staphylococcus aureus Reveals Distinct Lineages in Isolates from Miranda Donkeys (Equus asinus) and Their Handlers
title_full A One Health Approach Molecular Analysis of Staphylococcus aureus Reveals Distinct Lineages in Isolates from Miranda Donkeys (Equus asinus) and Their Handlers
title_fullStr A One Health Approach Molecular Analysis of Staphylococcus aureus Reveals Distinct Lineages in Isolates from Miranda Donkeys (Equus asinus) and Their Handlers
title_full_unstemmed A One Health Approach Molecular Analysis of Staphylococcus aureus Reveals Distinct Lineages in Isolates from Miranda Donkeys (Equus asinus) and Their Handlers
title_sort A One Health Approach Molecular Analysis of Staphylococcus aureus Reveals Distinct Lineages in Isolates from Miranda Donkeys (Equus asinus) and Their Handlers
author Silva, Vanessa
author_facet Silva, Vanessa
Alfarela, Cláudia
Caniça, Manuela
Manageiro, Vera
Nóvoa, Miguel
Leiva, Belen
Kress, Maria
Capelo, José Luís
Poeta, Patrícia
Igrejas, Gilberto
author_role author
author2 Alfarela, Cláudia
Caniça, Manuela
Manageiro, Vera
Nóvoa, Miguel
Leiva, Belen
Kress, Maria
Capelo, José Luís
Poeta, Patrícia
Igrejas, Gilberto
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv LAQV@REQUIMTE
DQ - Departamento de Química
RUN
dc.contributor.author.fl_str_mv Silva, Vanessa
Alfarela, Cláudia
Caniça, Manuela
Manageiro, Vera
Nóvoa, Miguel
Leiva, Belen
Kress, Maria
Capelo, José Luís
Poeta, Patrícia
Igrejas, Gilberto
dc.subject.por.fl_str_mv Animal-to-human transmission
CoNS
Donkeys
Staphylococcus aureus
Microbiology
Biochemistry
Pharmacology, Toxicology and Pharmaceutics(all)
Microbiology (medical)
Infectious Diseases
Pharmacology (medical)
SDG 3 - Good Health and Well-being
topic Animal-to-human transmission
CoNS
Donkeys
Staphylococcus aureus
Microbiology
Biochemistry
Pharmacology, Toxicology and Pharmaceutics(all)
Microbiology (medical)
Infectious Diseases
Pharmacology (medical)
SDG 3 - Good Health and Well-being
description This work was funded by the R&D Project CAREBIO2: Comparative assessment of antimicrobial resistance in environmental biofilms through proteomics—towards innovative thera-nostic biomarkers, with reference NORTE-01-0145-FEDER-030101, financed by the European Regional Development Fund (ERDF) through the Northern Regional Operational Program (NORTE 2020) and the Foundation for Science and Technology (FCT). projects UIDB/CVT/00772/2020 and LA/P/0059/2020 funded by the Portuguese Foundation for Science and Technology (FCT). Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
publishDate 2022
dc.date.none.fl_str_mv 2022-03-10
2022-03-10T00:00:00Z
2023-03-21T22:29:14Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/151036
url http://hdl.handle.net/10362/151036
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Silva, V., Alfarela, C., Caniça, M., Manageiro, V., Nóvoa, M., Leiva, B., Kress, M., Capelo, J. L., Poeta, P., & Igrejas, G. (2022). A One Health Approach Molecular Analysis of Staphylococcus aureus Reveals Distinct Lineages in Isolates from Miranda Donkeys (Equus asinus) and Their Handlers. Antibiotics, 11(3), [374]. https://doi.org/10.3390/antibiotics11030374
2079-6382
PURE: 56530058
https://doi.org/10.3390/antibiotics11030374
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 11
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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