The antibacterial and angiogenic effect of magnesium oxide in a hydroxyapatite bone substitute
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.22/18559 |
Resumo: | Bone graft infections are serious complications in orthopaedics and the growing resistance to antibiotics is increasing the need for antibacterial strategies. The use of magnesium oxide (MgO) is an interesting alternative since it possesses broad-spectrum antibacterial activity. Additionally, magnesium ions also play a role in bone regeneration, which makes MgO more appealing than other metal oxides. Therefore, a bone substitute composed of hydroxyapatite and MgO (HAp/MgO) spherical granules was developed using different sintering heat-treatment cycles to optimize its features. Depending on the sintering temperature, HAp/MgO spherical granules exhibited distinct surface topographies, mechanical strength and degradation profiles, that influenced the in vitro antibacterial activity and cytocompatibility. A proper balance between antibacterial activity and cytocompatibility was achieved with HAp/MgO spherical granules sintered at 1100 ºC. The presence of MgO in these granules was able to significantly reduce bacterial proliferation and simultaneously provide a suitable environment for osteoblasts growth. The angiogenic and inflammation potentials were also assessed using the in vivo chicken embryo chorioallantoic membrane (CAM) model and the spherical granules containing MgO stimulated angiogenesis without increasing inflammation. The outcomes of this study evidence a dual effect of MgO for bone regenerative applications making this material a promising antibacterial bone substitute. |
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The antibacterial and angiogenic effect of magnesium oxide in a hydroxyapatite bone substituteMagnesium oxideHydroxyapatite bone substituteBone graft infections are serious complications in orthopaedics and the growing resistance to antibiotics is increasing the need for antibacterial strategies. The use of magnesium oxide (MgO) is an interesting alternative since it possesses broad-spectrum antibacterial activity. Additionally, magnesium ions also play a role in bone regeneration, which makes MgO more appealing than other metal oxides. Therefore, a bone substitute composed of hydroxyapatite and MgO (HAp/MgO) spherical granules was developed using different sintering heat-treatment cycles to optimize its features. Depending on the sintering temperature, HAp/MgO spherical granules exhibited distinct surface topographies, mechanical strength and degradation profiles, that influenced the in vitro antibacterial activity and cytocompatibility. A proper balance between antibacterial activity and cytocompatibility was achieved with HAp/MgO spherical granules sintered at 1100 ºC. The presence of MgO in these granules was able to significantly reduce bacterial proliferation and simultaneously provide a suitable environment for osteoblasts growth. The angiogenic and inflammation potentials were also assessed using the in vivo chicken embryo chorioallantoic membrane (CAM) model and the spherical granules containing MgO stimulated angiogenesis without increasing inflammation. The outcomes of this study evidence a dual effect of MgO for bone regenerative applications making this material a promising antibacterial bone substitute.This work was financed by the project NoMIC2Bone (NORTE-01-0247-FEDER-017905) and project Biotherapies (NORTE-01-0145-FEDER-000012) supported by Norte Portugal Regional Operational Program (NORTE2020), under the PORTUGAL 2020 Partnership Agreement through the European Regional Development Fund (ERDF). Financial supported was also obtained from FEDER—Fundo Europeu de Desenvolvimento Regional funds through the COMPETE 2020—Operacional Programme for Competitiveness and Internationalization (POCI), PORTUGAL 2020, and by Portuguese funds through FCT/MCTES in the framework of the project “Institute for Research and Innovation in Health Sciences”(POCI-01-0145-FEDER-007274). Fundação para a Ciência e Tecnologia (FCT) is also acknowledged for Catarina Coelho’s PhD grant (SFRH/BDE/108971/2015). This work was also supported by the Applied Molecular Biosciences Unit-UCIBIO, which is financed by national funds from FCT/MCTES (UID/Multi/04378/2019). The authors would also want to acknowledge Rui Rocha from CEMUP for the contribution with SEM analysis and Dra. Rosário Soares from the University of Aveiro for the help with XRD data.Nature PortfolioRepositório Científico do Instituto Politécnico do PortoCoelho, Catarina C.Padrão, TatianaCosta, LauraPinto, Marta T .Costa, Paulo C.Domingues, Valentina F.Quadros, Paulo A.Monteiro, Fernando J.Sousa, Susana R.2021-09-27T10:59:56Z2020-112020-11-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/18559eng10.1038/s41598-020-76063-9info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T13:10:14Zoai:recipp.ipp.pt:10400.22/18559Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:38:04.160050Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
The antibacterial and angiogenic effect of magnesium oxide in a hydroxyapatite bone substitute |
title |
The antibacterial and angiogenic effect of magnesium oxide in a hydroxyapatite bone substitute |
spellingShingle |
The antibacterial and angiogenic effect of magnesium oxide in a hydroxyapatite bone substitute Coelho, Catarina C. Magnesium oxide Hydroxyapatite bone substitute |
title_short |
The antibacterial and angiogenic effect of magnesium oxide in a hydroxyapatite bone substitute |
title_full |
The antibacterial and angiogenic effect of magnesium oxide in a hydroxyapatite bone substitute |
title_fullStr |
The antibacterial and angiogenic effect of magnesium oxide in a hydroxyapatite bone substitute |
title_full_unstemmed |
The antibacterial and angiogenic effect of magnesium oxide in a hydroxyapatite bone substitute |
title_sort |
The antibacterial and angiogenic effect of magnesium oxide in a hydroxyapatite bone substitute |
author |
Coelho, Catarina C. |
author_facet |
Coelho, Catarina C. Padrão, Tatiana Costa, Laura Pinto, Marta T . Costa, Paulo C. Domingues, Valentina F. Quadros, Paulo A. Monteiro, Fernando J. Sousa, Susana R. |
author_role |
author |
author2 |
Padrão, Tatiana Costa, Laura Pinto, Marta T . Costa, Paulo C. Domingues, Valentina F. Quadros, Paulo A. Monteiro, Fernando J. Sousa, Susana R. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Politécnico do Porto |
dc.contributor.author.fl_str_mv |
Coelho, Catarina C. Padrão, Tatiana Costa, Laura Pinto, Marta T . Costa, Paulo C. Domingues, Valentina F. Quadros, Paulo A. Monteiro, Fernando J. Sousa, Susana R. |
dc.subject.por.fl_str_mv |
Magnesium oxide Hydroxyapatite bone substitute |
topic |
Magnesium oxide Hydroxyapatite bone substitute |
description |
Bone graft infections are serious complications in orthopaedics and the growing resistance to antibiotics is increasing the need for antibacterial strategies. The use of magnesium oxide (MgO) is an interesting alternative since it possesses broad-spectrum antibacterial activity. Additionally, magnesium ions also play a role in bone regeneration, which makes MgO more appealing than other metal oxides. Therefore, a bone substitute composed of hydroxyapatite and MgO (HAp/MgO) spherical granules was developed using different sintering heat-treatment cycles to optimize its features. Depending on the sintering temperature, HAp/MgO spherical granules exhibited distinct surface topographies, mechanical strength and degradation profiles, that influenced the in vitro antibacterial activity and cytocompatibility. A proper balance between antibacterial activity and cytocompatibility was achieved with HAp/MgO spherical granules sintered at 1100 ºC. The presence of MgO in these granules was able to significantly reduce bacterial proliferation and simultaneously provide a suitable environment for osteoblasts growth. The angiogenic and inflammation potentials were also assessed using the in vivo chicken embryo chorioallantoic membrane (CAM) model and the spherical granules containing MgO stimulated angiogenesis without increasing inflammation. The outcomes of this study evidence a dual effect of MgO for bone regenerative applications making this material a promising antibacterial bone substitute. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-11 2020-11-01T00:00:00Z 2021-09-27T10:59:56Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.22/18559 |
url |
http://hdl.handle.net/10400.22/18559 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1038/s41598-020-76063-9 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Nature Portfolio |
publisher.none.fl_str_mv |
Nature Portfolio |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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