Light modulates the lipidome of the photosynthetic sea slug Elysia timida

Detalhes bibliográficos
Autor(a) principal: Rey, Felisa
Data de Publicação: 2023
Outros Autores: Cartaxana, Paulo, Aveiro, Susana, Greenacre, Michael, Melo, Tânia, Domingues, Pedro, Domingues, M. Rosário, Cruz, Sónia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.1/19818
Resumo: Long-term kleptoplasty, the capability to retain functional stolen chloroplasts (kleptoplasts) for several weeks to months, has been shown in a handful of Sacoglossa sea slugs. One of these sea slugs is Elysia timida, endemic to the Mediterranean, which retains functional chloroplasts of the macroalga Acetabularia acetabulum. To understand how light modulates the lipidome of E. timida, sea slug specimens were subjected to two different 4-week light treatments: regular light and quasi-dark conditions. Lipidomic analyses were performed by HILIC-HR-ESIMS and MS/MS. Quasi-dark conditions caused a reduction in the amount of essential lipids for photosynthetic membranes, such as glycolipids, indicating high level of kleptoplast degradation under sub-optimal light conditions. However, maximum photosynthetic capacities (Fv/Fm) were identical in both light treatments (≈0.75), showing similar kleptoplast functionality and suggesting that older kleptoplasts were targeted for degradation. Although more stable, the phospholipidome showed differences between light treatments: the amount of certain lipid species of phosphatidylethanolamine (PE), phosphatidylinositol (PI), and phosphatidylglycerol (PG) decreased under quasi-dark conditions, while other lipid species of phosphatidylcholine (PC), PE and lyso-PE (LPE) increased. Quasi-dark conditions promoted a decrease in the relative abundance of polyunsaturated fatty acids. These results suggest a light-driven remodelling of the lipidome according to the functions of the different lipids and highlight the plasticity of polar lipids in the photosynthetic sea slug E. timida.
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spelling Light modulates the lipidome of the photosynthetic sea slug Elysia timidaFatty acidsKleptoplastyLipidomicsPhotosynthesisPolar lipidsSacoglossaLong-term kleptoplasty, the capability to retain functional stolen chloroplasts (kleptoplasts) for several weeks to months, has been shown in a handful of Sacoglossa sea slugs. One of these sea slugs is Elysia timida, endemic to the Mediterranean, which retains functional chloroplasts of the macroalga Acetabularia acetabulum. To understand how light modulates the lipidome of E. timida, sea slug specimens were subjected to two different 4-week light treatments: regular light and quasi-dark conditions. Lipidomic analyses were performed by HILIC-HR-ESIMS and MS/MS. Quasi-dark conditions caused a reduction in the amount of essential lipids for photosynthetic membranes, such as glycolipids, indicating high level of kleptoplast degradation under sub-optimal light conditions. However, maximum photosynthetic capacities (Fv/Fm) were identical in both light treatments (≈0.75), showing similar kleptoplast functionality and suggesting that older kleptoplasts were targeted for degradation. Although more stable, the phospholipidome showed differences between light treatments: the amount of certain lipid species of phosphatidylethanolamine (PE), phosphatidylinositol (PI), and phosphatidylglycerol (PG) decreased under quasi-dark conditions, while other lipid species of phosphatidylcholine (PC), PE and lyso-PE (LPE) increased. Quasi-dark conditions promoted a decrease in the relative abundance of polyunsaturated fatty acids. These results suggest a light-driven remodelling of the lipidome according to the functions of the different lipids and highlight the plasticity of polar lipids in the photosynthetic sea slug E. timida.LA/P/0094/2020; LISBOA-01-0145-FEDER-402-022125; CEECIND/01578/2020;ElsevierSapientiaRey, FelisaCartaxana, PauloAveiro, SusanaGreenacre, MichaelMelo, TâniaDomingues, PedroDomingues, M. RosárioCruz, Sónia2023-07-07T10:56:57Z20232023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/19818eng1388-1981/10.1016/j.bbalip.2022.159249info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:32:21Zoai:sapientia.ualg.pt:10400.1/19818Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:09:22.176273Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Light modulates the lipidome of the photosynthetic sea slug Elysia timida
title Light modulates the lipidome of the photosynthetic sea slug Elysia timida
spellingShingle Light modulates the lipidome of the photosynthetic sea slug Elysia timida
Rey, Felisa
Fatty acids
Kleptoplasty
Lipidomics
Photosynthesis
Polar lipids
Sacoglossa
title_short Light modulates the lipidome of the photosynthetic sea slug Elysia timida
title_full Light modulates the lipidome of the photosynthetic sea slug Elysia timida
title_fullStr Light modulates the lipidome of the photosynthetic sea slug Elysia timida
title_full_unstemmed Light modulates the lipidome of the photosynthetic sea slug Elysia timida
title_sort Light modulates the lipidome of the photosynthetic sea slug Elysia timida
author Rey, Felisa
author_facet Rey, Felisa
Cartaxana, Paulo
Aveiro, Susana
Greenacre, Michael
Melo, Tânia
Domingues, Pedro
Domingues, M. Rosário
Cruz, Sónia
author_role author
author2 Cartaxana, Paulo
Aveiro, Susana
Greenacre, Michael
Melo, Tânia
Domingues, Pedro
Domingues, M. Rosário
Cruz, Sónia
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Sapientia
dc.contributor.author.fl_str_mv Rey, Felisa
Cartaxana, Paulo
Aveiro, Susana
Greenacre, Michael
Melo, Tânia
Domingues, Pedro
Domingues, M. Rosário
Cruz, Sónia
dc.subject.por.fl_str_mv Fatty acids
Kleptoplasty
Lipidomics
Photosynthesis
Polar lipids
Sacoglossa
topic Fatty acids
Kleptoplasty
Lipidomics
Photosynthesis
Polar lipids
Sacoglossa
description Long-term kleptoplasty, the capability to retain functional stolen chloroplasts (kleptoplasts) for several weeks to months, has been shown in a handful of Sacoglossa sea slugs. One of these sea slugs is Elysia timida, endemic to the Mediterranean, which retains functional chloroplasts of the macroalga Acetabularia acetabulum. To understand how light modulates the lipidome of E. timida, sea slug specimens were subjected to two different 4-week light treatments: regular light and quasi-dark conditions. Lipidomic analyses were performed by HILIC-HR-ESIMS and MS/MS. Quasi-dark conditions caused a reduction in the amount of essential lipids for photosynthetic membranes, such as glycolipids, indicating high level of kleptoplast degradation under sub-optimal light conditions. However, maximum photosynthetic capacities (Fv/Fm) were identical in both light treatments (≈0.75), showing similar kleptoplast functionality and suggesting that older kleptoplasts were targeted for degradation. Although more stable, the phospholipidome showed differences between light treatments: the amount of certain lipid species of phosphatidylethanolamine (PE), phosphatidylinositol (PI), and phosphatidylglycerol (PG) decreased under quasi-dark conditions, while other lipid species of phosphatidylcholine (PC), PE and lyso-PE (LPE) increased. Quasi-dark conditions promoted a decrease in the relative abundance of polyunsaturated fatty acids. These results suggest a light-driven remodelling of the lipidome according to the functions of the different lipids and highlight the plasticity of polar lipids in the photosynthetic sea slug E. timida.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-07T10:56:57Z
2023
2023-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.1/19818
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dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1388-1981/
10.1016/j.bbalip.2022.159249
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eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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