Differences in the endophytic microbiome of olive cultivars infected by xylella fastidiosa across seasons
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/67135 |
Resumo: | The dynamics of <i>Xylella fastidiosa</i> infections in the context of the endophytic microbiome was studied in field-grown plants of the susceptible and resistant olive cultivars Kalamata and FS17. Whole metagenome shotgun sequencing (WMSS) coupled with 16S/ITS rRNA gene sequencing was carried out on the same trees at two different stages of the infections: In Spring 2017 when plants were almost symptomless and in Autumn 2018 when the trees of the susceptible cultivar clearly showed desiccations. The progression of the infections detected in both cultivars clearly unraveled that <i>Xylella</i> tends to occupy the whole ecological niche and suppresses the diversity of the endophytic microbiome. However, this trend was mitigated in the resistant cultivar FS17, harboring lower population sizes and therefore lower <i>Xylella</i> average abundance ratio over total bacteria, and a higher α-diversity. Host cultivar had a negligible effect on the community composition and no clear associations of a single taxon or microbial consortia with the resistance cultivar were found with both sequencing approaches, suggesting that the mechanisms of resistance likely reside on factors that are independent of the microbiome structure. Overall, <i>Proteobacteria</i>, <i>Actinobacteria</i>, <i>Firmicutes</i>, and <i>Bacteriodetes</i> dominated the bacterial microbiome while <i>Ascomycota</i> and <i>Basidiomycota</i> those of Fungi. |
id |
RCAP_cf01fac6abc181ddf26bf2faa2bed866 |
---|---|
oai_identifier_str |
oai:repositorium.sdum.uminho.pt:1822/67135 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Differences in the endophytic microbiome of olive cultivars infected by xylella fastidiosa across seasonsXylella fastidiosabacteria16S/ITS sequencingshotgun metagenomic sequencingkalamataFS17resistanceArchaeaFungiCiências Naturais::Ciências BiológicasScience & TechnologyThe dynamics of <i>Xylella fastidiosa</i> infections in the context of the endophytic microbiome was studied in field-grown plants of the susceptible and resistant olive cultivars Kalamata and FS17. Whole metagenome shotgun sequencing (WMSS) coupled with 16S/ITS rRNA gene sequencing was carried out on the same trees at two different stages of the infections: In Spring 2017 when plants were almost symptomless and in Autumn 2018 when the trees of the susceptible cultivar clearly showed desiccations. The progression of the infections detected in both cultivars clearly unraveled that <i>Xylella</i> tends to occupy the whole ecological niche and suppresses the diversity of the endophytic microbiome. However, this trend was mitigated in the resistant cultivar FS17, harboring lower population sizes and therefore lower <i>Xylella</i> average abundance ratio over total bacteria, and a higher α-diversity. Host cultivar had a negligible effect on the community composition and no clear associations of a single taxon or microbial consortia with the resistance cultivar were found with both sequencing approaches, suggesting that the mechanisms of resistance likely reside on factors that are independent of the microbiome structure. Overall, <i>Proteobacteria</i>, <i>Actinobacteria</i>, <i>Firmicutes</i>, and <i>Bacteriodetes</i> dominated the bacterial microbiome while <i>Ascomycota</i> and <i>Basidiomycota</i> those of Fungi.This research was funded by the EU H2020 Research Project XF-ACTORS "Xylella Fastidiosa Active Containment Through a multidisciplinary-Oriented Research Strategy" (Grant Agreement 727987) and by the Mountain Research Center-CIMO (UIDB/00690/2020) and BioISI (UIDB/04046/2020). The Funding Agencies are not responsible for any use that may be made of the information it contains.Multidisciplinary Digital Publishing InstituteUniversidade do MinhoGiampetruzzi, AnnalisaBaptista, PaulaMorelli, MassimilianoCameirão, CristinaLino-Neto, T.Costa, DanielaD’Attoma, GiusyAbou Kubaa, RaiedAltamura, GiuseppeSaponari, MariaPereira, José AlbertoSaldarelli, Pasquale20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/67135engGiampetruzzi, A.; Baptista, P.; Morelli, M.; Cameirão, C.; Lino Neto, T.; Costa, D.; D’Attoma, G.; Abou Kubaa, R.; Altamura, G.; Saponari, M.; Pereira, J.A.; Saldarelli, P. Differences in the Endophytic Microbiome of Olive Cultivars Infected by Xylella fastidiosa across Seasons. Pathogens 2020, 9, 723. DOI: 10.3390/pathogens90907232076-081710.3390/pathogens9090723https://www.mdpi.com/2076-0817/9/9/723info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:05:24Zoai:repositorium.sdum.uminho.pt:1822/67135Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:55:49.473147Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Differences in the endophytic microbiome of olive cultivars infected by xylella fastidiosa across seasons |
title |
Differences in the endophytic microbiome of olive cultivars infected by xylella fastidiosa across seasons |
spellingShingle |
Differences in the endophytic microbiome of olive cultivars infected by xylella fastidiosa across seasons Giampetruzzi, Annalisa Xylella fastidiosa bacteria 16S/ITS sequencing shotgun metagenomic sequencing kalamata FS17 resistance Archaea Fungi Ciências Naturais::Ciências Biológicas Science & Technology |
title_short |
Differences in the endophytic microbiome of olive cultivars infected by xylella fastidiosa across seasons |
title_full |
Differences in the endophytic microbiome of olive cultivars infected by xylella fastidiosa across seasons |
title_fullStr |
Differences in the endophytic microbiome of olive cultivars infected by xylella fastidiosa across seasons |
title_full_unstemmed |
Differences in the endophytic microbiome of olive cultivars infected by xylella fastidiosa across seasons |
title_sort |
Differences in the endophytic microbiome of olive cultivars infected by xylella fastidiosa across seasons |
author |
Giampetruzzi, Annalisa |
author_facet |
Giampetruzzi, Annalisa Baptista, Paula Morelli, Massimiliano Cameirão, Cristina Lino-Neto, T. Costa, Daniela D’Attoma, Giusy Abou Kubaa, Raied Altamura, Giuseppe Saponari, Maria Pereira, José Alberto Saldarelli, Pasquale |
author_role |
author |
author2 |
Baptista, Paula Morelli, Massimiliano Cameirão, Cristina Lino-Neto, T. Costa, Daniela D’Attoma, Giusy Abou Kubaa, Raied Altamura, Giuseppe Saponari, Maria Pereira, José Alberto Saldarelli, Pasquale |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Giampetruzzi, Annalisa Baptista, Paula Morelli, Massimiliano Cameirão, Cristina Lino-Neto, T. Costa, Daniela D’Attoma, Giusy Abou Kubaa, Raied Altamura, Giuseppe Saponari, Maria Pereira, José Alberto Saldarelli, Pasquale |
dc.subject.por.fl_str_mv |
Xylella fastidiosa bacteria 16S/ITS sequencing shotgun metagenomic sequencing kalamata FS17 resistance Archaea Fungi Ciências Naturais::Ciências Biológicas Science & Technology |
topic |
Xylella fastidiosa bacteria 16S/ITS sequencing shotgun metagenomic sequencing kalamata FS17 resistance Archaea Fungi Ciências Naturais::Ciências Biológicas Science & Technology |
description |
The dynamics of <i>Xylella fastidiosa</i> infections in the context of the endophytic microbiome was studied in field-grown plants of the susceptible and resistant olive cultivars Kalamata and FS17. Whole metagenome shotgun sequencing (WMSS) coupled with 16S/ITS rRNA gene sequencing was carried out on the same trees at two different stages of the infections: In Spring 2017 when plants were almost symptomless and in Autumn 2018 when the trees of the susceptible cultivar clearly showed desiccations. The progression of the infections detected in both cultivars clearly unraveled that <i>Xylella</i> tends to occupy the whole ecological niche and suppresses the diversity of the endophytic microbiome. However, this trend was mitigated in the resistant cultivar FS17, harboring lower population sizes and therefore lower <i>Xylella</i> average abundance ratio over total bacteria, and a higher α-diversity. Host cultivar had a negligible effect on the community composition and no clear associations of a single taxon or microbial consortia with the resistance cultivar were found with both sequencing approaches, suggesting that the mechanisms of resistance likely reside on factors that are independent of the microbiome structure. Overall, <i>Proteobacteria</i>, <i>Actinobacteria</i>, <i>Firmicutes</i>, and <i>Bacteriodetes</i> dominated the bacterial microbiome while <i>Ascomycota</i> and <i>Basidiomycota</i> those of Fungi. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020 2020-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/67135 |
url |
https://hdl.handle.net/1822/67135 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Giampetruzzi, A.; Baptista, P.; Morelli, M.; Cameirão, C.; Lino Neto, T.; Costa, D.; D’Attoma, G.; Abou Kubaa, R.; Altamura, G.; Saponari, M.; Pereira, J.A.; Saldarelli, P. Differences in the Endophytic Microbiome of Olive Cultivars Infected by Xylella fastidiosa across Seasons. Pathogens 2020, 9, 723. DOI: 10.3390/pathogens9090723 2076-0817 10.3390/pathogens9090723 https://www.mdpi.com/2076-0817/9/9/723 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute |
publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799132344556191744 |