Evidence of Air and Surface Contamination with SARS-CoV-2 in a Major Hospital in Portugal
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/10216/151510 |
Resumo: | As the third wave of the COVID-19 pandemic hit Portugal, it forced the country to reintro-duce lockdown measures due to hospitals reaching their full capacities. Under these circumstances, environmental contamination by SARS-CoV-2 in different areas of one of Portugal’s major Hospitals was assessed between 21 January and 11 February 2021. Air samples (n = 44) were collected from eleven different areas of the Hospital (four COVID-19 and seven non-COVID-19 areas) using Coriolis® µ and Coriolis® Compact cyclone air sampling devices. Surface sampling was also performed (n = 17) on four areas (one COVID-19 and three non-COVID-19 areas). RNA extraction followed by a one-step RT-qPCR adapted for quantitative purposes were performed. Of the 44 air samples, two were positive for SARS-CoV-2 RNA (6575 copies/m3 and 6662.5 copies/m3, respectively). Of the 17 surface samples, three were positive for SARS-CoV-2 RNA (200.6 copies/cm2, 179.2 copies/cm2, and 201.7 copies/cm2, respectively). SARS-CoV-2 environmental contamination was found both in air and on surfaces in both COVID-19 and non-COVID-19 areas. Moreover, our results suggest that longer collection sessions are needed to detect point contaminations. This reinforces the need to remain cautious at all times, not only when in close contact with infected individuals. Hand hygiene and other standard transmission-prevention guidelines should be continuously followed to avoid nosocomial COVID-19. © 2022 by the authors. Licensee MDPI, Basel, Switzerland. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Evidence of Air and Surface Contamination with SARS-CoV-2 in a Major Hospital in PortugalAir samples; Environmental contamination; SARS-CoV-2; Surface samplesAs the third wave of the COVID-19 pandemic hit Portugal, it forced the country to reintro-duce lockdown measures due to hospitals reaching their full capacities. Under these circumstances, environmental contamination by SARS-CoV-2 in different areas of one of Portugal’s major Hospitals was assessed between 21 January and 11 February 2021. Air samples (n = 44) were collected from eleven different areas of the Hospital (four COVID-19 and seven non-COVID-19 areas) using Coriolis® µ and Coriolis® Compact cyclone air sampling devices. Surface sampling was also performed (n = 17) on four areas (one COVID-19 and three non-COVID-19 areas). RNA extraction followed by a one-step RT-qPCR adapted for quantitative purposes were performed. Of the 44 air samples, two were positive for SARS-CoV-2 RNA (6575 copies/m3 and 6662.5 copies/m3, respectively). Of the 17 surface samples, three were positive for SARS-CoV-2 RNA (200.6 copies/cm2, 179.2 copies/cm2, and 201.7 copies/cm2, respectively). SARS-CoV-2 environmental contamination was found both in air and on surfaces in both COVID-19 and non-COVID-19 areas. Moreover, our results suggest that longer collection sessions are needed to detect point contaminations. This reinforces the need to remain cautious at all times, not only when in close contact with infected individuals. Hand hygiene and other standard transmission-prevention guidelines should be continuously followed to avoid nosocomial COVID-19. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.MDPI20222022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10216/151510eng1660-46011661-782710.3390/ijerph19010525da Silva, PGGonçalves, JLopes, AIBEsteves, NABamba, GEENascimento, MSJBranco, PTBSSoares, RRGSousa, SIVMesquita, JRinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-29T15:32:01Zoai:repositorio-aberto.up.pt:10216/151510Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:25:53.948963Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Evidence of Air and Surface Contamination with SARS-CoV-2 in a Major Hospital in Portugal |
title |
Evidence of Air and Surface Contamination with SARS-CoV-2 in a Major Hospital in Portugal |
spellingShingle |
Evidence of Air and Surface Contamination with SARS-CoV-2 in a Major Hospital in Portugal da Silva, PG Air samples; Environmental contamination; SARS-CoV-2; Surface samples |
title_short |
Evidence of Air and Surface Contamination with SARS-CoV-2 in a Major Hospital in Portugal |
title_full |
Evidence of Air and Surface Contamination with SARS-CoV-2 in a Major Hospital in Portugal |
title_fullStr |
Evidence of Air and Surface Contamination with SARS-CoV-2 in a Major Hospital in Portugal |
title_full_unstemmed |
Evidence of Air and Surface Contamination with SARS-CoV-2 in a Major Hospital in Portugal |
title_sort |
Evidence of Air and Surface Contamination with SARS-CoV-2 in a Major Hospital in Portugal |
author |
da Silva, PG |
author_facet |
da Silva, PG Gonçalves, J Lopes, AIB Esteves, NA Bamba, GEE Nascimento, MSJ Branco, PTBS Soares, RRG Sousa, SIV Mesquita, JR |
author_role |
author |
author2 |
Gonçalves, J Lopes, AIB Esteves, NA Bamba, GEE Nascimento, MSJ Branco, PTBS Soares, RRG Sousa, SIV Mesquita, JR |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
da Silva, PG Gonçalves, J Lopes, AIB Esteves, NA Bamba, GEE Nascimento, MSJ Branco, PTBS Soares, RRG Sousa, SIV Mesquita, JR |
dc.subject.por.fl_str_mv |
Air samples; Environmental contamination; SARS-CoV-2; Surface samples |
topic |
Air samples; Environmental contamination; SARS-CoV-2; Surface samples |
description |
As the third wave of the COVID-19 pandemic hit Portugal, it forced the country to reintro-duce lockdown measures due to hospitals reaching their full capacities. Under these circumstances, environmental contamination by SARS-CoV-2 in different areas of one of Portugal’s major Hospitals was assessed between 21 January and 11 February 2021. Air samples (n = 44) were collected from eleven different areas of the Hospital (four COVID-19 and seven non-COVID-19 areas) using Coriolis® µ and Coriolis® Compact cyclone air sampling devices. Surface sampling was also performed (n = 17) on four areas (one COVID-19 and three non-COVID-19 areas). RNA extraction followed by a one-step RT-qPCR adapted for quantitative purposes were performed. Of the 44 air samples, two were positive for SARS-CoV-2 RNA (6575 copies/m3 and 6662.5 copies/m3, respectively). Of the 17 surface samples, three were positive for SARS-CoV-2 RNA (200.6 copies/cm2, 179.2 copies/cm2, and 201.7 copies/cm2, respectively). SARS-CoV-2 environmental contamination was found both in air and on surfaces in both COVID-19 and non-COVID-19 areas. Moreover, our results suggest that longer collection sessions are needed to detect point contaminations. This reinforces the need to remain cautious at all times, not only when in close contact with infected individuals. Hand hygiene and other standard transmission-prevention guidelines should be continuously followed to avoid nosocomial COVID-19. © 2022 by the authors. Licensee MDPI, Basel, Switzerland. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022 2022-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/10216/151510 |
url |
https://hdl.handle.net/10216/151510 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1660-4601 1661-7827 10.3390/ijerph19010525 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799136172868370433 |