PCR Detection of Toxoplasma gondii in European Wild Rabbit (Oryctolagus cuniculus) from Portugal

Detalhes bibliográficos
Autor(a) principal: Coelho, Catarina
Data de Publicação: 2020
Outros Autores: Vieira-Pinto, Madalena, Vilares, Anabela, Gargaté, Maria João, Rodrigues, Manuela, Cardoso, Luís, Lopes, Ana Patrícia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/7636
Resumo: This article belongs to the Special Issue Parasitic Diseases from Wild Animals with Emphasis in Zoonotic Infections
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spelling PCR Detection of Toxoplasma gondii in European Wild Rabbit (Oryctolagus cuniculus) from PortugalEuropean Wild RabbitOryctolagus cuniculusPortugalToxoplasma GondiiPolymerase Chain ReactionSurface Antigen 2Infecções Sistémicas e ZoonosesThis article belongs to the Special Issue Parasitic Diseases from Wild Animals with Emphasis in Zoonotic InfectionsWildlife plays an important role in the epidemiological cycle of Toxoplasma gondii. The European wild rabbit (Oryctolagus cuniculus) can be a source of infection to wild and domestic hosts, including human beings. Additionally, as an herbivorous animal, the European wild rabbit may also be a sentinel of environmental contamination with T. gondii and, consequently, an indicator of the potential transmission of this parasite. The purpose of the present work was to detect T. gondii DNA in European wild rabbit from central Portugal, as well as the possible implications for public health. Heart and diaphragm samples were obtained from 28 rabbits hunted in central Portugal. Nested PCR separately amplified the 5' and 3' ends of the surface antigen 2 (SAG2) gene. T. gondii DNA was detected in 19 out of the 28 sampled animals, resulting in a prevalence of 67.9%. These results show that T. gondii infection occurs in European wild rabbit and therefore may pose a potential risk for humans if consumed as raw or undercooked meat. Measures should be taken in order to prevent infection by this zoonotic parasite and for the conservation of wildlife. To the best of our knowledge, this is the first study performed by means of PCR on T. gondii in European wild rabbit meat samples.This research was funded by Fundação para a Ciência e Tecnologia (FCT), project UIDB/CVT/00772/2020.MDPIRepositório Científico do Instituto Nacional de SaúdeCoelho, CatarinaVieira-Pinto, MadalenaVilares, AnabelaGargaté, Maria JoãoRodrigues, ManuelaCardoso, LuísLopes, Ana Patrícia2021-04-05T15:56:06Z2020-12-042020-12-04T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/7636engMicroorganisms. 2020 Dec 4;8(12):1926. doi: 10.3390/microorganisms8121926.2076-260710.3390/microorganisms8121926info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:42:07Zoai:repositorio.insa.pt:10400.18/7636Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:42:16.452657Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv PCR Detection of Toxoplasma gondii in European Wild Rabbit (Oryctolagus cuniculus) from Portugal
title PCR Detection of Toxoplasma gondii in European Wild Rabbit (Oryctolagus cuniculus) from Portugal
spellingShingle PCR Detection of Toxoplasma gondii in European Wild Rabbit (Oryctolagus cuniculus) from Portugal
Coelho, Catarina
European Wild Rabbit
Oryctolagus cuniculus
Portugal
Toxoplasma Gondii
Polymerase Chain Reaction
Surface Antigen 2
Infecções Sistémicas e Zoonoses
title_short PCR Detection of Toxoplasma gondii in European Wild Rabbit (Oryctolagus cuniculus) from Portugal
title_full PCR Detection of Toxoplasma gondii in European Wild Rabbit (Oryctolagus cuniculus) from Portugal
title_fullStr PCR Detection of Toxoplasma gondii in European Wild Rabbit (Oryctolagus cuniculus) from Portugal
title_full_unstemmed PCR Detection of Toxoplasma gondii in European Wild Rabbit (Oryctolagus cuniculus) from Portugal
title_sort PCR Detection of Toxoplasma gondii in European Wild Rabbit (Oryctolagus cuniculus) from Portugal
author Coelho, Catarina
author_facet Coelho, Catarina
Vieira-Pinto, Madalena
Vilares, Anabela
Gargaté, Maria João
Rodrigues, Manuela
Cardoso, Luís
Lopes, Ana Patrícia
author_role author
author2 Vieira-Pinto, Madalena
Vilares, Anabela
Gargaté, Maria João
Rodrigues, Manuela
Cardoso, Luís
Lopes, Ana Patrícia
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Coelho, Catarina
Vieira-Pinto, Madalena
Vilares, Anabela
Gargaté, Maria João
Rodrigues, Manuela
Cardoso, Luís
Lopes, Ana Patrícia
dc.subject.por.fl_str_mv European Wild Rabbit
Oryctolagus cuniculus
Portugal
Toxoplasma Gondii
Polymerase Chain Reaction
Surface Antigen 2
Infecções Sistémicas e Zoonoses
topic European Wild Rabbit
Oryctolagus cuniculus
Portugal
Toxoplasma Gondii
Polymerase Chain Reaction
Surface Antigen 2
Infecções Sistémicas e Zoonoses
description This article belongs to the Special Issue Parasitic Diseases from Wild Animals with Emphasis in Zoonotic Infections
publishDate 2020
dc.date.none.fl_str_mv 2020-12-04
2020-12-04T00:00:00Z
2021-04-05T15:56:06Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/7636
url http://hdl.handle.net/10400.18/7636
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Microorganisms. 2020 Dec 4;8(12):1926. doi: 10.3390/microorganisms8121926.
2076-2607
10.3390/microorganisms8121926
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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