Clonal Changes in the Pneumococcal Population Carried by Portuguese Children during Six Years of Private Use of the 13-Valent Pneumococcal Conjugate Vaccine
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2023 |
| Outros Autores: | , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/158550 |
Resumo: | Funding Information: Editor Max Maurin, UJF-Grenoble 1, CHU Grenoble Copyright © 2023 Candeias et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. Address correspondence to Raquel Sá-Leão, rsaleao@itqb.unl.pt. The authors declare a conflict of interest. This work was partially supported by Investigator Initiated Grants WI182109 and WI230921 from Pfizer Portugal (to R.S.-L.), LISBOA-01-0145-FEDER (Microbiologia Molecular, Estrutural e Celular, funded by FEDER through COMPETE2020 - Programa Operacional Competitividade e Internacionalização), and Fundação para a Ciência e a Tecnologia (C.C., S.F., S.H., were supported by PhD fellowships PD/BD/148434/2019, PD/BD/105732/2014, and PD/BD/128365/2017, respectively). Received 27 July 2022 Accepted 25 February 2023 Published 22 March 2023 Funding Information: This work was partially supported by Investigator Initiated Grants WI182109 and WI230921 from Pfizer Portugal (to R.S.-L.), LISBOA-01-0145-FEDER (Microbiologia Molecular, Estrutural e Celular, funded by FEDER through COMPETE2020—Programa Operacional Competitividade e Internacionalização), and Fundação para a Ciência e a Tecnologia (C.C., S.F., and S.H. were supported by PhD fellowships PD/BD/148434/ 2019, PD/BD/105732/2014, and PD/BD/128365/2017, respectively). Publisher Copyright: Copyright © 2023 Candeias et al. |
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Clonal Changes in the Pneumococcal Population Carried by Portuguese Children during Six Years of Private Use of the 13-Valent Pneumococcal Conjugate Vaccinethe Relative Contribution of Clonal Expansion, Clonal Emergence, and Capsular Switch EventsMLSTserotypeStreptococcus pneumoniaevaccinePhysiologyEcologyImmunology and Microbiology(all)GeneticsMicrobiology (medical)Cell BiologyInfectious DiseasesSDG 3 - Good Health and Well-beingFunding Information: Editor Max Maurin, UJF-Grenoble 1, CHU Grenoble Copyright © 2023 Candeias et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. Address correspondence to Raquel Sá-Leão, rsaleao@itqb.unl.pt. The authors declare a conflict of interest. This work was partially supported by Investigator Initiated Grants WI182109 and WI230921 from Pfizer Portugal (to R.S.-L.), LISBOA-01-0145-FEDER (Microbiologia Molecular, Estrutural e Celular, funded by FEDER through COMPETE2020 - Programa Operacional Competitividade e Internacionalização), and Fundação para a Ciência e a Tecnologia (C.C., S.F., S.H., were supported by PhD fellowships PD/BD/148434/2019, PD/BD/105732/2014, and PD/BD/128365/2017, respectively). Received 27 July 2022 Accepted 25 February 2023 Published 22 March 2023 Funding Information: This work was partially supported by Investigator Initiated Grants WI182109 and WI230921 from Pfizer Portugal (to R.S.-L.), LISBOA-01-0145-FEDER (Microbiologia Molecular, Estrutural e Celular, funded by FEDER through COMPETE2020—Programa Operacional Competitividade e Internacionalização), and Fundação para a Ciência e a Tecnologia (C.C., S.F., and S.H. were supported by PhD fellowships PD/BD/148434/ 2019, PD/BD/105732/2014, and PD/BD/128365/2017, respectively). Publisher Copyright: Copyright © 2023 Candeias et al.In Portugal, the 13-valent pneumococcal conjugate vaccine (PCV13) was available for private use from 2010 to 2015 and it was introduced in the National Immunization Program in 2015. We have reported that private use of PCV13 led to extensive serotype replacement and an increase in antimicrobial susceptibility among pneumococci carried by healthy children. We investigated which clonal changes concurred with these observations. A total of 657 pneumococcal strains, representative of a collection of 2,615 isolates, were genotyped by multilocus sequence typing (MLST). The isolates were recovered in 2009 to 2010 (pre-PCV13), 2011 to 2012 (early PCV13), and 2015 to 2016 (late PCV13) from children attending day care centers in two regions of Portugal (one urban, one rural). One-hundred seventy-one sequence types (STs) were identified, corresponding to 18 clonal complexes (CCs) and 58 singletons. Most CCs (n = 17) and several singletons (n = 16) were found in both regions, indicating that they were geographically disseminated. Clonal complexes expressing PCV13 serotypes in circulation in the late PCV13 period were a subset of the ones identified in the pre-PCV13 period and were often associated with antimicrobial resistance. Among those, the most frequent in both regions was CC179, a multidrug-resistant clone of serotype 19F. Serotype replacement, following PCV13 use, was mainly due to expansion of the susceptible lineages expressing non-PCV13 serotypes already in circulation in the pre-PCV13 period. The emergence of ST53, associated with serotype 8, a major cause of disease in several European countries, was observed in the rural region. Potential capsular switching events, unrelated to PCV13 use, were detected. This study improves our understanding of changes triggered by the private use of PCV13 in Portugal. IMPORTANCE Streptococcus pneumoniae (pneumococcus) is a major human respiratory pathogen linked with high morbidity, mortality, and health care-associated costs worldwide. This bacterium often colonizes asymptomatically healthy children. Colonization is a prerequisite for disease and is also essential for transmission between individuals. The 13-valent pneumococcal conjugate vaccine targets 13 of 101 capsular types of pneumococci described to date. This vaccine not only prevents pneumococcal disease but also impacts colonization by decreasing the carriage of vaccine serotypes. Consequently, serotype replacement occurs. The clonal changes occurring during serotype replacement may be due to various mechanisms, such as clonal expansion, emergence, extinction, or capsular switch (vaccine escape). This study shows that in Portugal, the use of PCV13 has led to significant changes in clonal composition and that these were mainly due to the clonal expansion of lineages expressing serotypes not included in the vaccine.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNCandeias, CatarinaFélix, SofiaHandem, Sarade Lencastre, HermíniaSá-Leão, Raquel2023-09-30T22:22:15Z2023-032023-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/158550eng2165-0497PURE: 72697879https://doi.org/10.1128/spectrum.02909-22info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:41:06Zoai:run.unl.pt:10362/158550Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:57:12.621Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Clonal Changes in the Pneumococcal Population Carried by Portuguese Children during Six Years of Private Use of the 13-Valent Pneumococcal Conjugate Vaccine the Relative Contribution of Clonal Expansion, Clonal Emergence, and Capsular Switch Events |
| title |
Clonal Changes in the Pneumococcal Population Carried by Portuguese Children during Six Years of Private Use of the 13-Valent Pneumococcal Conjugate Vaccine |
| spellingShingle |
Clonal Changes in the Pneumococcal Population Carried by Portuguese Children during Six Years of Private Use of the 13-Valent Pneumococcal Conjugate Vaccine Candeias, Catarina MLST serotype Streptococcus pneumoniae vaccine Physiology Ecology Immunology and Microbiology(all) Genetics Microbiology (medical) Cell Biology Infectious Diseases SDG 3 - Good Health and Well-being |
| title_short |
Clonal Changes in the Pneumococcal Population Carried by Portuguese Children during Six Years of Private Use of the 13-Valent Pneumococcal Conjugate Vaccine |
| title_full |
Clonal Changes in the Pneumococcal Population Carried by Portuguese Children during Six Years of Private Use of the 13-Valent Pneumococcal Conjugate Vaccine |
| title_fullStr |
Clonal Changes in the Pneumococcal Population Carried by Portuguese Children during Six Years of Private Use of the 13-Valent Pneumococcal Conjugate Vaccine |
| title_full_unstemmed |
Clonal Changes in the Pneumococcal Population Carried by Portuguese Children during Six Years of Private Use of the 13-Valent Pneumococcal Conjugate Vaccine |
| title_sort |
Clonal Changes in the Pneumococcal Population Carried by Portuguese Children during Six Years of Private Use of the 13-Valent Pneumococcal Conjugate Vaccine |
| author |
Candeias, Catarina |
| author_facet |
Candeias, Catarina Félix, Sofia Handem, Sara de Lencastre, Hermínia Sá-Leão, Raquel |
| author_role |
author |
| author2 |
Félix, Sofia Handem, Sara de Lencastre, Hermínia Sá-Leão, Raquel |
| author2_role |
author author author author |
| dc.contributor.none.fl_str_mv |
Instituto de Tecnologia Química e Biológica António Xavier (ITQB) RUN |
| dc.contributor.author.fl_str_mv |
Candeias, Catarina Félix, Sofia Handem, Sara de Lencastre, Hermínia Sá-Leão, Raquel |
| dc.subject.por.fl_str_mv |
MLST serotype Streptococcus pneumoniae vaccine Physiology Ecology Immunology and Microbiology(all) Genetics Microbiology (medical) Cell Biology Infectious Diseases SDG 3 - Good Health and Well-being |
| topic |
MLST serotype Streptococcus pneumoniae vaccine Physiology Ecology Immunology and Microbiology(all) Genetics Microbiology (medical) Cell Biology Infectious Diseases SDG 3 - Good Health and Well-being |
| description |
Funding Information: Editor Max Maurin, UJF-Grenoble 1, CHU Grenoble Copyright © 2023 Candeias et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. Address correspondence to Raquel Sá-Leão, rsaleao@itqb.unl.pt. The authors declare a conflict of interest. This work was partially supported by Investigator Initiated Grants WI182109 and WI230921 from Pfizer Portugal (to R.S.-L.), LISBOA-01-0145-FEDER (Microbiologia Molecular, Estrutural e Celular, funded by FEDER through COMPETE2020 - Programa Operacional Competitividade e Internacionalização), and Fundação para a Ciência e a Tecnologia (C.C., S.F., S.H., were supported by PhD fellowships PD/BD/148434/2019, PD/BD/105732/2014, and PD/BD/128365/2017, respectively). Received 27 July 2022 Accepted 25 February 2023 Published 22 March 2023 Funding Information: This work was partially supported by Investigator Initiated Grants WI182109 and WI230921 from Pfizer Portugal (to R.S.-L.), LISBOA-01-0145-FEDER (Microbiologia Molecular, Estrutural e Celular, funded by FEDER through COMPETE2020—Programa Operacional Competitividade e Internacionalização), and Fundação para a Ciência e a Tecnologia (C.C., S.F., and S.H. were supported by PhD fellowships PD/BD/148434/ 2019, PD/BD/105732/2014, and PD/BD/128365/2017, respectively). Publisher Copyright: Copyright © 2023 Candeias et al. |
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2023 |
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2023-09-30T22:22:15Z 2023-03 2023-03-01T00:00:00Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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http://hdl.handle.net/10362/158550 |
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2165-0497 PURE: 72697879 https://doi.org/10.1128/spectrum.02909-22 |
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