Does predation exacerbate the risk of endosymbiont loss in heat stressed hermatypic corals?
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/157004 |
Resumo: | Ocean warming has been a major driver of coral reef bleaching and mass mortality. Coupled to other biotic pressures, corals’ ability for acclimatization and adaptation may become compromised. Here, we tested the combined effects of warming scenarios (26, 30, and 32°C) and predation (wound vs. no wound) in coral health condition (paleness, bleaching, and mortality), cellular stress responses (heat shock protein 70 kDa Hsp70, total ubiquitin Ub, and total antioxidant capacity TAC), and physiological state (integrated biomarker response index, IBR) of seven Scleractinian coral species, after being exposed for 60 days. Results show that although temperature was the main factor driving coral health condition, thermotolerant species (Galaxea fascicularis, Psammocora contigua, and Turbinaria reniformis) displayed increased paleness, bleaching, and mortality in predation treatments at high temperature, whereas thermosensitive species (Acropora tenuis, Echinopora lamellosa, and Montipora capricornis brown and green morphotypes) all died at 32°C, regardless of predation condition. At the molecular level, results show that there were significant main and interactive effects of species, temperature, and predation in the biomarkers assessed. Temperature affected Hsp70, Ub, and TAC, evidencing the role of protein folding and turnover, as well as reactive oxygen species scavenging in heat stress management. Predation increased Hsp70 and Ub, suggesting the activation of the pro-phenoloxidase system and cytokine activity, whereas the combination of both stressors mainly affected TAC during moderate stress and Ub under severe stress, suggesting that redox balance and defense of homeostasis are crucial in tissue repair at high temperature. IBR levels showed an increasing trend at 32°C in predated coral fragments (although non-significant). We conclude that coral responses to the combination of high temperature and predation pressure display high inter-species variability, but these stressors may pose a higher risk of endosymbiont loss, depending on species physiology and stress intensity. |
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Does predation exacerbate the risk of endosymbiont loss in heat stressed hermatypic corals?Molecular cues provide insights into species-specific health outcomes in a multi-stressor oceanbiomarkerscoral reefsendosymbiont losshealth conditionmolecular responsesocean warmingpredationtissue woundsPhysiologyPhysiology (medical)Ocean warming has been a major driver of coral reef bleaching and mass mortality. Coupled to other biotic pressures, corals’ ability for acclimatization and adaptation may become compromised. Here, we tested the combined effects of warming scenarios (26, 30, and 32°C) and predation (wound vs. no wound) in coral health condition (paleness, bleaching, and mortality), cellular stress responses (heat shock protein 70 kDa Hsp70, total ubiquitin Ub, and total antioxidant capacity TAC), and physiological state (integrated biomarker response index, IBR) of seven Scleractinian coral species, after being exposed for 60 days. Results show that although temperature was the main factor driving coral health condition, thermotolerant species (Galaxea fascicularis, Psammocora contigua, and Turbinaria reniformis) displayed increased paleness, bleaching, and mortality in predation treatments at high temperature, whereas thermosensitive species (Acropora tenuis, Echinopora lamellosa, and Montipora capricornis brown and green morphotypes) all died at 32°C, regardless of predation condition. At the molecular level, results show that there were significant main and interactive effects of species, temperature, and predation in the biomarkers assessed. Temperature affected Hsp70, Ub, and TAC, evidencing the role of protein folding and turnover, as well as reactive oxygen species scavenging in heat stress management. Predation increased Hsp70 and Ub, suggesting the activation of the pro-phenoloxidase system and cytokine activity, whereas the combination of both stressors mainly affected TAC during moderate stress and Ub under severe stress, suggesting that redox balance and defense of homeostasis are crucial in tissue repair at high temperature. IBR levels showed an increasing trend at 32°C in predated coral fragments (although non-significant). We conclude that coral responses to the combination of high temperature and predation pressure display high inter-species variability, but these stressors may pose a higher risk of endosymbiont loss, depending on species physiology and stress intensity.UCIBIO - Applied Molecular Biosciences UnitRUNMadeira, CarolinaDias, MartaFerreira, AnaGouveia, RaúlCabral, Henrique N.Diniz, Mário S.Vinagre, Catarina2023-08-29T22:12:17Z2022-03-012022-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article19application/pdfhttp://hdl.handle.net/10362/157004eng1664-042XPURE: 42508301https://doi.org/10.3389/fphys.2022.801672info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:39:14Zoai:run.unl.pt:10362/157004Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:56:30.685178Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Does predation exacerbate the risk of endosymbiont loss in heat stressed hermatypic corals? Molecular cues provide insights into species-specific health outcomes in a multi-stressor ocean |
title |
Does predation exacerbate the risk of endosymbiont loss in heat stressed hermatypic corals? |
spellingShingle |
Does predation exacerbate the risk of endosymbiont loss in heat stressed hermatypic corals? Madeira, Carolina biomarkers coral reefs endosymbiont loss health condition molecular responses ocean warming predation tissue wounds Physiology Physiology (medical) |
title_short |
Does predation exacerbate the risk of endosymbiont loss in heat stressed hermatypic corals? |
title_full |
Does predation exacerbate the risk of endosymbiont loss in heat stressed hermatypic corals? |
title_fullStr |
Does predation exacerbate the risk of endosymbiont loss in heat stressed hermatypic corals? |
title_full_unstemmed |
Does predation exacerbate the risk of endosymbiont loss in heat stressed hermatypic corals? |
title_sort |
Does predation exacerbate the risk of endosymbiont loss in heat stressed hermatypic corals? |
author |
Madeira, Carolina |
author_facet |
Madeira, Carolina Dias, Marta Ferreira, Ana Gouveia, Raúl Cabral, Henrique N. Diniz, Mário S. Vinagre, Catarina |
author_role |
author |
author2 |
Dias, Marta Ferreira, Ana Gouveia, Raúl Cabral, Henrique N. Diniz, Mário S. Vinagre, Catarina |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
UCIBIO - Applied Molecular Biosciences Unit RUN |
dc.contributor.author.fl_str_mv |
Madeira, Carolina Dias, Marta Ferreira, Ana Gouveia, Raúl Cabral, Henrique N. Diniz, Mário S. Vinagre, Catarina |
dc.subject.por.fl_str_mv |
biomarkers coral reefs endosymbiont loss health condition molecular responses ocean warming predation tissue wounds Physiology Physiology (medical) |
topic |
biomarkers coral reefs endosymbiont loss health condition molecular responses ocean warming predation tissue wounds Physiology Physiology (medical) |
description |
Ocean warming has been a major driver of coral reef bleaching and mass mortality. Coupled to other biotic pressures, corals’ ability for acclimatization and adaptation may become compromised. Here, we tested the combined effects of warming scenarios (26, 30, and 32°C) and predation (wound vs. no wound) in coral health condition (paleness, bleaching, and mortality), cellular stress responses (heat shock protein 70 kDa Hsp70, total ubiquitin Ub, and total antioxidant capacity TAC), and physiological state (integrated biomarker response index, IBR) of seven Scleractinian coral species, after being exposed for 60 days. Results show that although temperature was the main factor driving coral health condition, thermotolerant species (Galaxea fascicularis, Psammocora contigua, and Turbinaria reniformis) displayed increased paleness, bleaching, and mortality in predation treatments at high temperature, whereas thermosensitive species (Acropora tenuis, Echinopora lamellosa, and Montipora capricornis brown and green morphotypes) all died at 32°C, regardless of predation condition. At the molecular level, results show that there were significant main and interactive effects of species, temperature, and predation in the biomarkers assessed. Temperature affected Hsp70, Ub, and TAC, evidencing the role of protein folding and turnover, as well as reactive oxygen species scavenging in heat stress management. Predation increased Hsp70 and Ub, suggesting the activation of the pro-phenoloxidase system and cytokine activity, whereas the combination of both stressors mainly affected TAC during moderate stress and Ub under severe stress, suggesting that redox balance and defense of homeostasis are crucial in tissue repair at high temperature. IBR levels showed an increasing trend at 32°C in predated coral fragments (although non-significant). We conclude that coral responses to the combination of high temperature and predation pressure display high inter-species variability, but these stressors may pose a higher risk of endosymbiont loss, depending on species physiology and stress intensity. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-03-01 2022-03-01T00:00:00Z 2023-08-29T22:12:17Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/157004 |
url |
http://hdl.handle.net/10362/157004 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1664-042X PURE: 42508301 https://doi.org/10.3389/fphys.2022.801672 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
19 application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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