Methanolic extract of the herb ononis spinosa L. is an antifungal agent with no cytotoxicity to primary human cells

Detalhes bibliográficos
Autor(a) principal: Stojković, Dejan
Data de Publicação: 2018
Outros Autores: Dias, Maria Inês, Drakulić, Danijela, Barros, Lillian, Stevanović, Milena, Ferreira, Isabel C.F.R., Soković, Marina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10198/23417
Resumo: Ononis spinosa L. is a plant traditionally used as folk remedy. There are numerous studies regarding chemical constituents and health beneficial properties of Ononidis Radix. The following study was designed to investigate chemical composition and antifungal potential of the methanolic extract obtained from the O. spinosa L. herb. Chemical analyses regarding phenolic compounds of O. spinosa were performed by liquid chromatography with mass spectrometry (LC-DAD-ESI/MSn). Antifungal activity, antibiofilm properties and antifungal mode of action of the extract were evaluated, as well as cytotoxicity. Chemical analyses revealed the presence of flavonoids, isoflavonoids and phenolic acids in O. spinosa, with kaempherol-O-hexoside-pentoside being the most abundant compound (5.1 mg/g extract). Methanolic extract was active against all of the tested microfungi with Penicillium aurantiogriseum being the most sensitive to the extract inhibitory effect at 0.02 mg/mL; and effectively inhibited biofilms formed by Candida strains. Minimum fungicidal concentrations of extract rose in the presence of ergosterol and leakage of cellular components was detected. The extract showed no cytotoxicity to human gingival fibroblast (HGF-1) cells. This study significantly contributes to overall knowledge about medicinal potential of O. spinosa herbal extract and enlightens previously unrevealed properties. O. spinosa aerial parts seem to be an interesting candidate for the development of antifungal preparations, non-toxic to human cells.
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spelling Methanolic extract of the herb ononis spinosa L. is an antifungal agent with no cytotoxicity to primary human cellsAntibiofilmCytotoxicityO. spinosaOnonis spinosa L. is a plant traditionally used as folk remedy. There are numerous studies regarding chemical constituents and health beneficial properties of Ononidis Radix. The following study was designed to investigate chemical composition and antifungal potential of the methanolic extract obtained from the O. spinosa L. herb. Chemical analyses regarding phenolic compounds of O. spinosa were performed by liquid chromatography with mass spectrometry (LC-DAD-ESI/MSn). Antifungal activity, antibiofilm properties and antifungal mode of action of the extract were evaluated, as well as cytotoxicity. Chemical analyses revealed the presence of flavonoids, isoflavonoids and phenolic acids in O. spinosa, with kaempherol-O-hexoside-pentoside being the most abundant compound (5.1 mg/g extract). Methanolic extract was active against all of the tested microfungi with Penicillium aurantiogriseum being the most sensitive to the extract inhibitory effect at 0.02 mg/mL; and effectively inhibited biofilms formed by Candida strains. Minimum fungicidal concentrations of extract rose in the presence of ergosterol and leakage of cellular components was detected. The extract showed no cytotoxicity to human gingival fibroblast (HGF-1) cells. This study significantly contributes to overall knowledge about medicinal potential of O. spinosa herbal extract and enlightens previously unrevealed properties. O. spinosa aerial parts seem to be an interesting candidate for the development of antifungal preparations, non-toxic to human cells.This work has been supported by Ministry of Education, Science and Technological Development of Republic of Serbia (451-03-68/2020-14/200007 and 451-03-68/2020-14/200042). The authors are grateful to the Foundation for Science and Technology (FCT, Portugal) and FEDER under Program PT2020 for financial support to CIMO (UIDB/00690/2020); national funding by FCT, P.I., through the institutional scientific employment program-contract for M.I. Dias and L. Barros; to FEDER-Interreg España-Portugal program for financial support through the project 0377_Iberphenol_6_E and TRANSCoLAB 0612_TRANS_CO_LAB_2_P.Biblioteca Digital do IPBStojković, DejanDias, Maria InêsDrakulić, DanijelaBarros, LillianStevanović, MilenaFerreira, Isabel C.F.R.Soković, Marina2018-01-19T10:00:00Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/23417engStojković, Dejan; Dias, Maria Inês; Drakulić, Danijela; Barros, Lillian; Stevanović, Milena; Ferreira, Isabel C.F.R.; Soković, Marina (2020). Methanolic extract of the herb ononis spinosa L. Is an antifungal agent with no cytotoxicity to primary human cells. Pharmaceuticals. ISSN 1424-8247. 13:4, p. 1-1310.3390/ph13040078info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:52:22Zoai:bibliotecadigital.ipb.pt:10198/23417Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:14:24.928587Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Methanolic extract of the herb ononis spinosa L. is an antifungal agent with no cytotoxicity to primary human cells
title Methanolic extract of the herb ononis spinosa L. is an antifungal agent with no cytotoxicity to primary human cells
spellingShingle Methanolic extract of the herb ononis spinosa L. is an antifungal agent with no cytotoxicity to primary human cells
Stojković, Dejan
Antibiofilm
Cytotoxicity
O. spinosa
title_short Methanolic extract of the herb ononis spinosa L. is an antifungal agent with no cytotoxicity to primary human cells
title_full Methanolic extract of the herb ononis spinosa L. is an antifungal agent with no cytotoxicity to primary human cells
title_fullStr Methanolic extract of the herb ononis spinosa L. is an antifungal agent with no cytotoxicity to primary human cells
title_full_unstemmed Methanolic extract of the herb ononis spinosa L. is an antifungal agent with no cytotoxicity to primary human cells
title_sort Methanolic extract of the herb ononis spinosa L. is an antifungal agent with no cytotoxicity to primary human cells
author Stojković, Dejan
author_facet Stojković, Dejan
Dias, Maria Inês
Drakulić, Danijela
Barros, Lillian
Stevanović, Milena
Ferreira, Isabel C.F.R.
Soković, Marina
author_role author
author2 Dias, Maria Inês
Drakulić, Danijela
Barros, Lillian
Stevanović, Milena
Ferreira, Isabel C.F.R.
Soković, Marina
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Biblioteca Digital do IPB
dc.contributor.author.fl_str_mv Stojković, Dejan
Dias, Maria Inês
Drakulić, Danijela
Barros, Lillian
Stevanović, Milena
Ferreira, Isabel C.F.R.
Soković, Marina
dc.subject.por.fl_str_mv Antibiofilm
Cytotoxicity
O. spinosa
topic Antibiofilm
Cytotoxicity
O. spinosa
description Ononis spinosa L. is a plant traditionally used as folk remedy. There are numerous studies regarding chemical constituents and health beneficial properties of Ononidis Radix. The following study was designed to investigate chemical composition and antifungal potential of the methanolic extract obtained from the O. spinosa L. herb. Chemical analyses regarding phenolic compounds of O. spinosa were performed by liquid chromatography with mass spectrometry (LC-DAD-ESI/MSn). Antifungal activity, antibiofilm properties and antifungal mode of action of the extract were evaluated, as well as cytotoxicity. Chemical analyses revealed the presence of flavonoids, isoflavonoids and phenolic acids in O. spinosa, with kaempherol-O-hexoside-pentoside being the most abundant compound (5.1 mg/g extract). Methanolic extract was active against all of the tested microfungi with Penicillium aurantiogriseum being the most sensitive to the extract inhibitory effect at 0.02 mg/mL; and effectively inhibited biofilms formed by Candida strains. Minimum fungicidal concentrations of extract rose in the presence of ergosterol and leakage of cellular components was detected. The extract showed no cytotoxicity to human gingival fibroblast (HGF-1) cells. This study significantly contributes to overall knowledge about medicinal potential of O. spinosa herbal extract and enlightens previously unrevealed properties. O. spinosa aerial parts seem to be an interesting candidate for the development of antifungal preparations, non-toxic to human cells.
publishDate 2018
dc.date.none.fl_str_mv 2018-01-19T10:00:00Z
2020
2020-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10198/23417
url http://hdl.handle.net/10198/23417
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Stojković, Dejan; Dias, Maria Inês; Drakulić, Danijela; Barros, Lillian; Stevanović, Milena; Ferreira, Isabel C.F.R.; Soković, Marina (2020). Methanolic extract of the herb ononis spinosa L. Is an antifungal agent with no cytotoxicity to primary human cells. Pharmaceuticals. ISSN 1424-8247. 13:4, p. 1-13
10.3390/ph13040078
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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