A versatile qPCR for diagnosis of leporid gammaherpesvirus 5 using Evagreen® or Taqman® Technologies

Detalhes bibliográficos
Autor(a) principal: Abade Dos Santos, F.A.
Data de Publicação: 2021
Outros Autores: Carvalho, Carina L., Peleteiro, Maria C., Parra, Francisco, Duarte, M. D.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/21447
Resumo: ABSTRACT - In late 2019, the first herpesvirus in the genus Lepus, named leporid gammaherpesvirus 5 (LeHV-5) was described. At the time, herpetic typical lesions were observed in hares infected by the myxoma virus, which is known to induce immunosuppression. Though the real impact of LeHV-5 is still poorly understood, since it affects reproduction, it poses an additional threat to the already fragile populations of Iberian hare, demanding prevalence investigations. In this article, we describe the first quantitative molecular method for LeHV-5 detection, using either Taqman or the EvaGreen systems. This method has excellent sensitivity and specificity, it is able to detect 2.1 copies of LeHV-5 DNA and was validated with an internal control targeting the 18S rRNA gene, allowing monitoring extraction and PCR amplification efficiencies.
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spelling A versatile qPCR for diagnosis of leporid gammaherpesvirus 5 using Evagreen® or Taqman® TechnologiesHerpesvirusGammaherpesvirusLeporid gammaherpesvirus 5Lepus granatensisIberian hareReproductionqPCRABSTRACT - In late 2019, the first herpesvirus in the genus Lepus, named leporid gammaherpesvirus 5 (LeHV-5) was described. At the time, herpetic typical lesions were observed in hares infected by the myxoma virus, which is known to induce immunosuppression. Though the real impact of LeHV-5 is still poorly understood, since it affects reproduction, it poses an additional threat to the already fragile populations of Iberian hare, demanding prevalence investigations. In this article, we describe the first quantitative molecular method for LeHV-5 detection, using either Taqman or the EvaGreen systems. This method has excellent sensitivity and specificity, it is able to detect 2.1 copies of LeHV-5 DNA and was validated with an internal control targeting the 18S rRNA gene, allowing monitoring extraction and PCR amplification efficiencies.MDPIRepositório da Universidade de LisboaAbade Dos Santos, F.A.Carvalho, Carina L.Peleteiro, Maria C.Parra, FranciscoDuarte, M. D.2021-06-11T14:49:18Z2021-04-202021-04-20T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/21447engAbade dos Santos FA, Carvalho CL, Peleteiro MC, Parra F, Duarte MD. 2021. A Versatile qPCR for Diagnosis of Leporid Gammaherpesvirus 5 Using Evagreen® or Taqman® Technologies. Viruses 13(4):715. Doi: https://doi.org/10.3390/v130407151999-491510.3390/v13040715info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:50:53Zoai:www.repository.utl.pt:10400.5/21447Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:06:01.985986Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A versatile qPCR for diagnosis of leporid gammaherpesvirus 5 using Evagreen® or Taqman® Technologies
title A versatile qPCR for diagnosis of leporid gammaherpesvirus 5 using Evagreen® or Taqman® Technologies
spellingShingle A versatile qPCR for diagnosis of leporid gammaherpesvirus 5 using Evagreen® or Taqman® Technologies
Abade Dos Santos, F.A.
Herpesvirus
Gammaherpesvirus
Leporid gammaherpesvirus 5
Lepus granatensis
Iberian hare
Reproduction
qPCR
title_short A versatile qPCR for diagnosis of leporid gammaherpesvirus 5 using Evagreen® or Taqman® Technologies
title_full A versatile qPCR for diagnosis of leporid gammaherpesvirus 5 using Evagreen® or Taqman® Technologies
title_fullStr A versatile qPCR for diagnosis of leporid gammaherpesvirus 5 using Evagreen® or Taqman® Technologies
title_full_unstemmed A versatile qPCR for diagnosis of leporid gammaherpesvirus 5 using Evagreen® or Taqman® Technologies
title_sort A versatile qPCR for diagnosis of leporid gammaherpesvirus 5 using Evagreen® or Taqman® Technologies
author Abade Dos Santos, F.A.
author_facet Abade Dos Santos, F.A.
Carvalho, Carina L.
Peleteiro, Maria C.
Parra, Francisco
Duarte, M. D.
author_role author
author2 Carvalho, Carina L.
Peleteiro, Maria C.
Parra, Francisco
Duarte, M. D.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Abade Dos Santos, F.A.
Carvalho, Carina L.
Peleteiro, Maria C.
Parra, Francisco
Duarte, M. D.
dc.subject.por.fl_str_mv Herpesvirus
Gammaherpesvirus
Leporid gammaherpesvirus 5
Lepus granatensis
Iberian hare
Reproduction
qPCR
topic Herpesvirus
Gammaherpesvirus
Leporid gammaherpesvirus 5
Lepus granatensis
Iberian hare
Reproduction
qPCR
description ABSTRACT - In late 2019, the first herpesvirus in the genus Lepus, named leporid gammaherpesvirus 5 (LeHV-5) was described. At the time, herpetic typical lesions were observed in hares infected by the myxoma virus, which is known to induce immunosuppression. Though the real impact of LeHV-5 is still poorly understood, since it affects reproduction, it poses an additional threat to the already fragile populations of Iberian hare, demanding prevalence investigations. In this article, we describe the first quantitative molecular method for LeHV-5 detection, using either Taqman or the EvaGreen systems. This method has excellent sensitivity and specificity, it is able to detect 2.1 copies of LeHV-5 DNA and was validated with an internal control targeting the 18S rRNA gene, allowing monitoring extraction and PCR amplification efficiencies.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-11T14:49:18Z
2021-04-20
2021-04-20T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/21447
url http://hdl.handle.net/10400.5/21447
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Abade dos Santos FA, Carvalho CL, Peleteiro MC, Parra F, Duarte MD. 2021. A Versatile qPCR for Diagnosis of Leporid Gammaherpesvirus 5 Using Evagreen® or Taqman® Technologies. Viruses 13(4):715. Doi: https://doi.org/10.3390/v13040715
1999-4915
10.3390/v13040715
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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