Appraisal of a new potential antioxidants-rich nutraceutical ingredient from chestnut shells through in-vivo assays – A targeted metabolomic approach in phenolic compounds

Detalhes bibliográficos
Autor(a) principal: Pinto, Diana
Data de Publicação: 2023
Outros Autores: Almeida, Andreia, López-Yerena, Anallely, Pinto, Soraia, Sarmento, Bruno, Lamuela-Raventós, Rosa, Vallverdú-Queralt, Anna, Delerue-Matos, Cristina, Rodrigues, Francisca
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.22/22493
Resumo: Chestnut (Castanea sativa) shells (CSS) are a source of bioactive compounds with well demonstrated in-vitro antioxidant properties. Nevertheless, no in-vivo studies have already evaluated this effect. This study evaluated the effects of the oral daily administration of an eco-friendly CSS extract (50 and 100 mg/kg per body weight (b. w.)) to rats regarding in-vivo antioxidant activity, glucose and lipids levels, and metabolomic profiling of poly- phenols by LC-ESI-LTQ-Orbitrap-MS. The results demonstrated the in-vivo antioxidant properties in the animals liver, kidney and blood serum, as well as protective effects against hemolysis and rising of blood glucose and lipids levels. New insights on metabolomic profiling of polyphenols proved their absorption and further biotransformation by phase I (hydrogenation and hydroxylation) and II reactions (glucuronidation, methylation and sulfation). This is the first study that attempted to validate a novel nutraceutical ingredient extracted from CSS by in-vivo assays, corroborating the outcomes screened by in-vitro assays
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spelling Appraisal of a new potential antioxidants-rich nutraceutical ingredient from chestnut shells through in-vivo assays – A targeted metabolomic approach in phenolic compoundsCastanea sativaSubcritical water extractionPolyphenolsIn-vivo studyNutraceuticalMetabolomicChestnut (Castanea sativa) shells (CSS) are a source of bioactive compounds with well demonstrated in-vitro antioxidant properties. Nevertheless, no in-vivo studies have already evaluated this effect. This study evaluated the effects of the oral daily administration of an eco-friendly CSS extract (50 and 100 mg/kg per body weight (b. w.)) to rats regarding in-vivo antioxidant activity, glucose and lipids levels, and metabolomic profiling of poly- phenols by LC-ESI-LTQ-Orbitrap-MS. The results demonstrated the in-vivo antioxidant properties in the animals liver, kidney and blood serum, as well as protective effects against hemolysis and rising of blood glucose and lipids levels. New insights on metabolomic profiling of polyphenols proved their absorption and further biotransformation by phase I (hydrogenation and hydroxylation) and II reactions (glucuronidation, methylation and sulfation). This is the first study that attempted to validate a novel nutraceutical ingredient extracted from CSS by in-vivo assays, corroborating the outcomes screened by in-vitro assaysThe authors’ kindly thanks to Sortegel (Sortes, Portugal) for the samples. This work received financial support from national funds (UIDB/50006/2020), project PTDC/ASP-AGR/29277/2017 - Castanea sativa shells as a new source of active ingredients for Functional Food and Cosmetic applications: a sustainable approach, and project 5537 DRI, Sérvia 2020/21 from Portuguese-Serbia Bilateral Cooperation - Development of functional foods incorporating a chestnut shells extract obtained by subcritical water, supported by national funds by FCT / MCTES and co-supported by Fundo Europeu de Desenvolvimento Regional (FEDER) throughout COMPETE 2020 - Programa Operacional Competitividade e Internacionalização (POCI-01-0145-FEDER-029277). Diana Pinto (SFRH/BD/144534/2019) and Soraia Pinto (SFRH/BD/144719/2019) are thankful for their PhD grants financed by FCT/MCTES and POPH-QREN and supported by funds from European Union (EU) and Fundo Social Europeu (FSE) through Programa Operacional Regional Norte. Francisca Rodrigues is grateful for her contract (CEECIND/01886/2020) financed by FCT/MCTES—CEEC Individual 2020 Program Contract. Anna Vallverdú-Queralt thanks the Spanish Ministerio de Ciencia, Innovación y Universidades for the Ramon y Cajal contract (RYC-2016-19355).ElsevierRepositório Científico do Instituto Politécnico do PortoPinto, DianaAlmeida, AndreiaLópez-Yerena, AnallelyPinto, SoraiaSarmento, BrunoLamuela-Raventós, RosaVallverdú-Queralt, AnnaDelerue-Matos, CristinaRodrigues, Francisca2023-03-15T10:21:00Z20232023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/22493eng0308-814610.1016/j.foodchem.2022.134546info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-22T01:46:54Zoai:recipp.ipp.pt:10400.22/22493Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:45:00.252416Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Appraisal of a new potential antioxidants-rich nutraceutical ingredient from chestnut shells through in-vivo assays – A targeted metabolomic approach in phenolic compounds
title Appraisal of a new potential antioxidants-rich nutraceutical ingredient from chestnut shells through in-vivo assays – A targeted metabolomic approach in phenolic compounds
spellingShingle Appraisal of a new potential antioxidants-rich nutraceutical ingredient from chestnut shells through in-vivo assays – A targeted metabolomic approach in phenolic compounds
Pinto, Diana
Castanea sativa
Subcritical water extraction
Polyphenols
In-vivo study
Nutraceutical
Metabolomic
title_short Appraisal of a new potential antioxidants-rich nutraceutical ingredient from chestnut shells through in-vivo assays – A targeted metabolomic approach in phenolic compounds
title_full Appraisal of a new potential antioxidants-rich nutraceutical ingredient from chestnut shells through in-vivo assays – A targeted metabolomic approach in phenolic compounds
title_fullStr Appraisal of a new potential antioxidants-rich nutraceutical ingredient from chestnut shells through in-vivo assays – A targeted metabolomic approach in phenolic compounds
title_full_unstemmed Appraisal of a new potential antioxidants-rich nutraceutical ingredient from chestnut shells through in-vivo assays – A targeted metabolomic approach in phenolic compounds
title_sort Appraisal of a new potential antioxidants-rich nutraceutical ingredient from chestnut shells through in-vivo assays – A targeted metabolomic approach in phenolic compounds
author Pinto, Diana
author_facet Pinto, Diana
Almeida, Andreia
López-Yerena, Anallely
Pinto, Soraia
Sarmento, Bruno
Lamuela-Raventós, Rosa
Vallverdú-Queralt, Anna
Delerue-Matos, Cristina
Rodrigues, Francisca
author_role author
author2 Almeida, Andreia
López-Yerena, Anallely
Pinto, Soraia
Sarmento, Bruno
Lamuela-Raventós, Rosa
Vallverdú-Queralt, Anna
Delerue-Matos, Cristina
Rodrigues, Francisca
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Politécnico do Porto
dc.contributor.author.fl_str_mv Pinto, Diana
Almeida, Andreia
López-Yerena, Anallely
Pinto, Soraia
Sarmento, Bruno
Lamuela-Raventós, Rosa
Vallverdú-Queralt, Anna
Delerue-Matos, Cristina
Rodrigues, Francisca
dc.subject.por.fl_str_mv Castanea sativa
Subcritical water extraction
Polyphenols
In-vivo study
Nutraceutical
Metabolomic
topic Castanea sativa
Subcritical water extraction
Polyphenols
In-vivo study
Nutraceutical
Metabolomic
description Chestnut (Castanea sativa) shells (CSS) are a source of bioactive compounds with well demonstrated in-vitro antioxidant properties. Nevertheless, no in-vivo studies have already evaluated this effect. This study evaluated the effects of the oral daily administration of an eco-friendly CSS extract (50 and 100 mg/kg per body weight (b. w.)) to rats regarding in-vivo antioxidant activity, glucose and lipids levels, and metabolomic profiling of poly- phenols by LC-ESI-LTQ-Orbitrap-MS. The results demonstrated the in-vivo antioxidant properties in the animals liver, kidney and blood serum, as well as protective effects against hemolysis and rising of blood glucose and lipids levels. New insights on metabolomic profiling of polyphenols proved their absorption and further biotransformation by phase I (hydrogenation and hydroxylation) and II reactions (glucuronidation, methylation and sulfation). This is the first study that attempted to validate a novel nutraceutical ingredient extracted from CSS by in-vivo assays, corroborating the outcomes screened by in-vitro assays
publishDate 2023
dc.date.none.fl_str_mv 2023-03-15T10:21:00Z
2023
2023-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.22/22493
url http://hdl.handle.net/10400.22/22493
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0308-8146
10.1016/j.foodchem.2022.134546
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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