Bacteriophage cocktail-mediated inhibition of Pseudomonas aeruginosa biofilm on endotracheal tube surface
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/70860 |
Resumo: | Although different strategies to control biofilm formation on endotracheal tubes have been proposed, there are scarce scientific data on applying phages for both removing and preventing Pseudomonas aeruginosa biofilms on the device surface. Here, the anti-biofilm capacity of five bacteriophages was evaluated by a high content screening assay. We observed that biofilms were significantly reduced after phage treatment, especially in multidrug-resistant strains. Considering the anti-biofilm screens, two phages were selected as cocktail components, and the cocktail’s ability to prevent colonization of the endotracheal tube surface was tested in a dynamic biofilm model. Phage-coated tubes were challenged with different P. aeruginosa strains. The biofilm growth was monitored from 24 to 168 h by colony forming unit counting, metabolic activity assessment, and biofilm morphology observation. The phage cocktail promoted differences of bacterial colonization; nonetheless, the action was strain dependent. Phage cocktail coating did not promote substantial changes in metabolic activity. Scanning electron microscopy revealed a higher concentration of biofilm cells in control, while tower-like structures could be observed on phage cocktail-coated tubes. These results demonstrate that with the development of new coating strategies, phage therapy has potential in controlling the endotracheal tube-associated biofilm. |
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Bacteriophage cocktail-mediated inhibition of Pseudomonas aeruginosa biofilm on endotracheal tube surfacebacteriophagebiofilmPseudomonas aeruginosaendotracheal tubeScience & TechnologyAlthough different strategies to control biofilm formation on endotracheal tubes have been proposed, there are scarce scientific data on applying phages for both removing and preventing Pseudomonas aeruginosa biofilms on the device surface. Here, the anti-biofilm capacity of five bacteriophages was evaluated by a high content screening assay. We observed that biofilms were significantly reduced after phage treatment, especially in multidrug-resistant strains. Considering the anti-biofilm screens, two phages were selected as cocktail components, and the cocktail’s ability to prevent colonization of the endotracheal tube surface was tested in a dynamic biofilm model. Phage-coated tubes were challenged with different P. aeruginosa strains. The biofilm growth was monitored from 24 to 168 h by colony forming unit counting, metabolic activity assessment, and biofilm morphology observation. The phage cocktail promoted differences of bacterial colonization; nonetheless, the action was strain dependent. Phage cocktail coating did not promote substantial changes in metabolic activity. Scanning electron microscopy revealed a higher concentration of biofilm cells in control, while tower-like structures could be observed on phage cocktail-coated tubes. These results demonstrate that with the development of new coating strategies, phage therapy has potential in controlling the endotracheal tube-associated biofilm.This work was supported by the São Paulo Research Foundation (FAPESP) under the grants 2018/09757-0, 2019/13271-9 and 2020/03405-5, as well as the National Council for Scientific and Technological Development (CNPq) under the grant 405622/2018-0.info:eu-repo/semantics/publishedVersionMDPIUniversidade do MinhoOliveira, Viviane C.Macedo, Ana P.Melo, Luís Daniel RodriguesSantos, Sílvio Roberto BrancoHermann, Paula R. S.Silva-Lovato, Cláudia H.Paranhos, Helena F. O.Andrade, DeniseWatanabe, Evandro20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/70860engOliveira, Viviane C.; Macedo, Ana P.; Melo, Luís Daniel Rodrigues; Santos, Sílvio Roberto Branco; Hermann, Paula R. S.; Silva-Lovato, Cláudia H.; Paranhos, Helena F. O.; Andrade, Denise; Watanabe, Evandro, Bacteriophage cocktail-mediated inhibition of Pseudomonas aeruginosa biofilm on endotracheal tube surface. Antibiotics, 10(1), 78-78, 20212079-63822079-638210.3390/antibiotics10010078https://www.mdpi.com/2079-6382/10/1/78info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:09:56Zoai:repositorium.sdum.uminho.pt:1822/70860Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:01:27.634284Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Bacteriophage cocktail-mediated inhibition of Pseudomonas aeruginosa biofilm on endotracheal tube surface |
title |
Bacteriophage cocktail-mediated inhibition of Pseudomonas aeruginosa biofilm on endotracheal tube surface |
spellingShingle |
Bacteriophage cocktail-mediated inhibition of Pseudomonas aeruginosa biofilm on endotracheal tube surface Oliveira, Viviane C. bacteriophage biofilm Pseudomonas aeruginosa endotracheal tube Science & Technology |
title_short |
Bacteriophage cocktail-mediated inhibition of Pseudomonas aeruginosa biofilm on endotracheal tube surface |
title_full |
Bacteriophage cocktail-mediated inhibition of Pseudomonas aeruginosa biofilm on endotracheal tube surface |
title_fullStr |
Bacteriophage cocktail-mediated inhibition of Pseudomonas aeruginosa biofilm on endotracheal tube surface |
title_full_unstemmed |
Bacteriophage cocktail-mediated inhibition of Pseudomonas aeruginosa biofilm on endotracheal tube surface |
title_sort |
Bacteriophage cocktail-mediated inhibition of Pseudomonas aeruginosa biofilm on endotracheal tube surface |
author |
Oliveira, Viviane C. |
author_facet |
Oliveira, Viviane C. Macedo, Ana P. Melo, Luís Daniel Rodrigues Santos, Sílvio Roberto Branco Hermann, Paula R. S. Silva-Lovato, Cláudia H. Paranhos, Helena F. O. Andrade, Denise Watanabe, Evandro |
author_role |
author |
author2 |
Macedo, Ana P. Melo, Luís Daniel Rodrigues Santos, Sílvio Roberto Branco Hermann, Paula R. S. Silva-Lovato, Cláudia H. Paranhos, Helena F. O. Andrade, Denise Watanabe, Evandro |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Oliveira, Viviane C. Macedo, Ana P. Melo, Luís Daniel Rodrigues Santos, Sílvio Roberto Branco Hermann, Paula R. S. Silva-Lovato, Cláudia H. Paranhos, Helena F. O. Andrade, Denise Watanabe, Evandro |
dc.subject.por.fl_str_mv |
bacteriophage biofilm Pseudomonas aeruginosa endotracheal tube Science & Technology |
topic |
bacteriophage biofilm Pseudomonas aeruginosa endotracheal tube Science & Technology |
description |
Although different strategies to control biofilm formation on endotracheal tubes have been proposed, there are scarce scientific data on applying phages for both removing and preventing Pseudomonas aeruginosa biofilms on the device surface. Here, the anti-biofilm capacity of five bacteriophages was evaluated by a high content screening assay. We observed that biofilms were significantly reduced after phage treatment, especially in multidrug-resistant strains. Considering the anti-biofilm screens, two phages were selected as cocktail components, and the cocktail’s ability to prevent colonization of the endotracheal tube surface was tested in a dynamic biofilm model. Phage-coated tubes were challenged with different P. aeruginosa strains. The biofilm growth was monitored from 24 to 168 h by colony forming unit counting, metabolic activity assessment, and biofilm morphology observation. The phage cocktail promoted differences of bacterial colonization; nonetheless, the action was strain dependent. Phage cocktail coating did not promote substantial changes in metabolic activity. Scanning electron microscopy revealed a higher concentration of biofilm cells in control, while tower-like structures could be observed on phage cocktail-coated tubes. These results demonstrate that with the development of new coating strategies, phage therapy has potential in controlling the endotracheal tube-associated biofilm. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021 2021-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/70860 |
url |
http://hdl.handle.net/1822/70860 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Oliveira, Viviane C.; Macedo, Ana P.; Melo, Luís Daniel Rodrigues; Santos, Sílvio Roberto Branco; Hermann, Paula R. S.; Silva-Lovato, Cláudia H.; Paranhos, Helena F. O.; Andrade, Denise; Watanabe, Evandro, Bacteriophage cocktail-mediated inhibition of Pseudomonas aeruginosa biofilm on endotracheal tube surface. Antibiotics, 10(1), 78-78, 2021 2079-6382 2079-6382 10.3390/antibiotics10010078 https://www.mdpi.com/2079-6382/10/1/78 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132413123624960 |