Characterization of four defense-related genes up-regulated in root nodules of Casuarina glauca
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.13/5284 |
Resumo: | Actinorhizal plants are capable of high rates of nitrogen fixation, due to their capacity to establish a root nodule symbiosis with N2-fixing actinomycetes of the genus Frankia. Nodulation is an ontogenic process which requires a sequence of highly coordinated events. One of these mechanisms is the induction of defense-related events, whose precise role during nodulation is largely unknown. In order to contribute to the clarification of the involvement of defense-related genes during actinorhizal root-nodule symbiosis, we have analysed the differential expression of several genes with putative defense-related functions in Casuarina glauca nodules versus non inoculated roots. Four genes encoding a chitinase (CgChi1), a glutathione S-transferase (CgGst), a hairpin-inducible protein (CgHin1) and a peroxidase (CgPox4) were found to be up-regulated in mature nodules compared to roots. In order to find out to which extend were the encoded proteins involved in nodule protection, development or both, gene regulation studies in response to SA and wounding as well as phylogenetic analysis of the protein sequences were performed. These were further characterized through expression studies after SA-treatment and wounding, and by phylogenetic analysis. We suggest that CgChi1 and CgGst are involved in defense or microsymbiont control and CgPox4 is involved in nodule development. For CgHin1 the question “defense, development or both” remains open. |
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Characterization of four defense-related genes up-regulated in root nodules of Casuarina glaucaActinorhizal symbiosisCasuarina glaucaFrankiaDefenseChitinasesNHLGSTPeroxidases.Faculdade de Ciências da VidaActinorhizal plants are capable of high rates of nitrogen fixation, due to their capacity to establish a root nodule symbiosis with N2-fixing actinomycetes of the genus Frankia. Nodulation is an ontogenic process which requires a sequence of highly coordinated events. One of these mechanisms is the induction of defense-related events, whose precise role during nodulation is largely unknown. In order to contribute to the clarification of the involvement of defense-related genes during actinorhizal root-nodule symbiosis, we have analysed the differential expression of several genes with putative defense-related functions in Casuarina glauca nodules versus non inoculated roots. Four genes encoding a chitinase (CgChi1), a glutathione S-transferase (CgGst), a hairpin-inducible protein (CgHin1) and a peroxidase (CgPox4) were found to be up-regulated in mature nodules compared to roots. In order to find out to which extend were the encoded proteins involved in nodule protection, development or both, gene regulation studies in response to SA and wounding as well as phylogenetic analysis of the protein sequences were performed. These were further characterized through expression studies after SA-treatment and wounding, and by phylogenetic analysis. We suggest that CgChi1 and CgGst are involved in defense or microsymbiont control and CgPox4 is involved in nodule development. For CgHin1 the question “defense, development or both” remains open.SpringerDigitUMaSantos, PatríciaFortunato, AnaGraça, InêsMartins, Sandra MarinaGouveia, Maria ManuelaAuguy, FlorenceBogusz, DidierRicardo, Cândido Pereira PintoPawlowski, KatharinaRibeiro, Ana2023-07-24T09:32:55Z20092009-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.13/5284engSantos, P., Fortunato, A., Graça, I. et al. Characterization of four defense-related genes up-regulated in root nodules of Casuarina glauca . Symbiosis 50, 27–35 (2010). https://doi.org/10.1007/s13199-009-0031-010.1007/s13199-009-0031-0info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-30T07:05:44Zoai:digituma.uma.pt:10400.13/5284Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:10:13.215293Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Characterization of four defense-related genes up-regulated in root nodules of Casuarina glauca |
title |
Characterization of four defense-related genes up-regulated in root nodules of Casuarina glauca |
spellingShingle |
Characterization of four defense-related genes up-regulated in root nodules of Casuarina glauca Santos, Patrícia Actinorhizal symbiosis Casuarina glauca Frankia Defense Chitinases NHL GST Peroxidases . Faculdade de Ciências da Vida |
title_short |
Characterization of four defense-related genes up-regulated in root nodules of Casuarina glauca |
title_full |
Characterization of four defense-related genes up-regulated in root nodules of Casuarina glauca |
title_fullStr |
Characterization of four defense-related genes up-regulated in root nodules of Casuarina glauca |
title_full_unstemmed |
Characterization of four defense-related genes up-regulated in root nodules of Casuarina glauca |
title_sort |
Characterization of four defense-related genes up-regulated in root nodules of Casuarina glauca |
author |
Santos, Patrícia |
author_facet |
Santos, Patrícia Fortunato, Ana Graça, Inês Martins, Sandra Marina Gouveia, Maria Manuela Auguy, Florence Bogusz, Didier Ricardo, Cândido Pereira Pinto Pawlowski, Katharina Ribeiro, Ana |
author_role |
author |
author2 |
Fortunato, Ana Graça, Inês Martins, Sandra Marina Gouveia, Maria Manuela Auguy, Florence Bogusz, Didier Ricardo, Cândido Pereira Pinto Pawlowski, Katharina Ribeiro, Ana |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
DigitUMa |
dc.contributor.author.fl_str_mv |
Santos, Patrícia Fortunato, Ana Graça, Inês Martins, Sandra Marina Gouveia, Maria Manuela Auguy, Florence Bogusz, Didier Ricardo, Cândido Pereira Pinto Pawlowski, Katharina Ribeiro, Ana |
dc.subject.por.fl_str_mv |
Actinorhizal symbiosis Casuarina glauca Frankia Defense Chitinases NHL GST Peroxidases . Faculdade de Ciências da Vida |
topic |
Actinorhizal symbiosis Casuarina glauca Frankia Defense Chitinases NHL GST Peroxidases . Faculdade de Ciências da Vida |
description |
Actinorhizal plants are capable of high rates of nitrogen fixation, due to their capacity to establish a root nodule symbiosis with N2-fixing actinomycetes of the genus Frankia. Nodulation is an ontogenic process which requires a sequence of highly coordinated events. One of these mechanisms is the induction of defense-related events, whose precise role during nodulation is largely unknown. In order to contribute to the clarification of the involvement of defense-related genes during actinorhizal root-nodule symbiosis, we have analysed the differential expression of several genes with putative defense-related functions in Casuarina glauca nodules versus non inoculated roots. Four genes encoding a chitinase (CgChi1), a glutathione S-transferase (CgGst), a hairpin-inducible protein (CgHin1) and a peroxidase (CgPox4) were found to be up-regulated in mature nodules compared to roots. In order to find out to which extend were the encoded proteins involved in nodule protection, development or both, gene regulation studies in response to SA and wounding as well as phylogenetic analysis of the protein sequences were performed. These were further characterized through expression studies after SA-treatment and wounding, and by phylogenetic analysis. We suggest that CgChi1 and CgGst are involved in defense or microsymbiont control and CgPox4 is involved in nodule development. For CgHin1 the question “defense, development or both” remains open. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009 2009-01-01T00:00:00Z 2023-07-24T09:32:55Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.13/5284 |
url |
http://hdl.handle.net/10400.13/5284 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Santos, P., Fortunato, A., Graça, I. et al. Characterization of four defense-related genes up-regulated in root nodules of Casuarina glauca . Symbiosis 50, 27–35 (2010). https://doi.org/10.1007/s13199-009-0031-0 10.1007/s13199-009-0031-0 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133351902183424 |