Characterization of NS5A and NS5B resistance-associated substitutions from genotype 1 Hepatitis C Virus infected patients in a Portuguese cohort

Detalhes bibliográficos
Autor(a) principal: Brandão, Ruben
Data de Publicação: 2018
Outros Autores: Marcelino, Rute, Gonçalves, Fátima, Diogo, Isabel, Carvalho, Ana, Cabanas, Joaquim, Costa, Inês, Brogueira, Pedro, Ventura, Fernando, Miranda, Ana, Mansinho, Kamal, Gomes, Perpétua
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.26/30657
Resumo: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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spelling Characterization of NS5A and NS5B resistance-associated substitutions from genotype 1 Hepatitis C Virus infected patients in a Portuguese cohortHepatitis C virusDirect-acting antiviralsResistance-associated substitutionsNS5ANS5BThis is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.This study is focused on the prevalent NS5 coding region resistance-associated substitutions (RASs) in DAA-naive genotype (GT)1 HCV-infected patients and their potential impact on success rates. Plasma RNA from 81 GT1 HCV-infected patients was extracted prior to an in-house nested RT-PCR of the NS5 coding region, which is followed by Sanger population sequencing. NS5A RASs were present in 28.4% (23/81) of all GT1-infected patients with 9.9% (8/81) having the Y93C/H mutation. NS5B RASs showed a prevalence of 14.8% (12/81) and were only detected in GT1b. Overall 38.3% (31/81) of all GT1 HCV-infected patients presented baseline RASs. The obtained data supports the usefulness of resistance testing prior to treatment since a statistically significant association was found between treatment failure and the baseline presence of specific NS5 RASs known as Y93C/H (p = 0.04).MDPIRepositório ComumBrandão, RubenMarcelino, RuteGonçalves, FátimaDiogo, IsabelCarvalho, AnaCabanas, JoaquimCosta, InêsBrogueira, PedroVentura, FernandoMiranda, AnaMansinho, KamalGomes, Perpétua2019-12-20T16:14:10Z2018-04-01T00:00:00Z2018-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.26/30657engBrandão, R.; Marcelino, R.; Gonçalves, F.; Diogo, I.; Carvalho, A.; Cabanas, J.; Costa, I.; Brogueira, P.; Ventura, F.; Miranda, A.; Mansinho, K.; Gomes, P. Characterization of NS5A and NS5B Resistance-Associated Substitutions from Genotype 1 Hepatitis C Virus Infected Patients in a Portuguese Cohort. Viruses 2018, 10, 223.1999-491510.3390/v10050223info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-10-06T14:53:59Zoai:comum.rcaap.pt:10400.26/30657Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:09:44.555582Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Characterization of NS5A and NS5B resistance-associated substitutions from genotype 1 Hepatitis C Virus infected patients in a Portuguese cohort
title Characterization of NS5A and NS5B resistance-associated substitutions from genotype 1 Hepatitis C Virus infected patients in a Portuguese cohort
spellingShingle Characterization of NS5A and NS5B resistance-associated substitutions from genotype 1 Hepatitis C Virus infected patients in a Portuguese cohort
Brandão, Ruben
Hepatitis C virus
Direct-acting antivirals
Resistance-associated substitutions
NS5A
NS5B
title_short Characterization of NS5A and NS5B resistance-associated substitutions from genotype 1 Hepatitis C Virus infected patients in a Portuguese cohort
title_full Characterization of NS5A and NS5B resistance-associated substitutions from genotype 1 Hepatitis C Virus infected patients in a Portuguese cohort
title_fullStr Characterization of NS5A and NS5B resistance-associated substitutions from genotype 1 Hepatitis C Virus infected patients in a Portuguese cohort
title_full_unstemmed Characterization of NS5A and NS5B resistance-associated substitutions from genotype 1 Hepatitis C Virus infected patients in a Portuguese cohort
title_sort Characterization of NS5A and NS5B resistance-associated substitutions from genotype 1 Hepatitis C Virus infected patients in a Portuguese cohort
author Brandão, Ruben
author_facet Brandão, Ruben
Marcelino, Rute
Gonçalves, Fátima
Diogo, Isabel
Carvalho, Ana
Cabanas, Joaquim
Costa, Inês
Brogueira, Pedro
Ventura, Fernando
Miranda, Ana
Mansinho, Kamal
Gomes, Perpétua
author_role author
author2 Marcelino, Rute
Gonçalves, Fátima
Diogo, Isabel
Carvalho, Ana
Cabanas, Joaquim
Costa, Inês
Brogueira, Pedro
Ventura, Fernando
Miranda, Ana
Mansinho, Kamal
Gomes, Perpétua
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Comum
dc.contributor.author.fl_str_mv Brandão, Ruben
Marcelino, Rute
Gonçalves, Fátima
Diogo, Isabel
Carvalho, Ana
Cabanas, Joaquim
Costa, Inês
Brogueira, Pedro
Ventura, Fernando
Miranda, Ana
Mansinho, Kamal
Gomes, Perpétua
dc.subject.por.fl_str_mv Hepatitis C virus
Direct-acting antivirals
Resistance-associated substitutions
NS5A
NS5B
topic Hepatitis C virus
Direct-acting antivirals
Resistance-associated substitutions
NS5A
NS5B
description This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
publishDate 2018
dc.date.none.fl_str_mv 2018-04-01T00:00:00Z
2018-04-01T00:00:00Z
2019-12-20T16:14:10Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.26/30657
url http://hdl.handle.net/10400.26/30657
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brandão, R.; Marcelino, R.; Gonçalves, F.; Diogo, I.; Carvalho, A.; Cabanas, J.; Costa, I.; Brogueira, P.; Ventura, F.; Miranda, A.; Mansinho, K.; Gomes, P. Characterization of NS5A and NS5B Resistance-Associated Substitutions from Genotype 1 Hepatitis C Virus Infected Patients in a Portuguese Cohort. Viruses 2018, 10, 223.
1999-4915
10.3390/v10050223
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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