Angiotensin II mediates catecholamine and neuropeptide Y secretion in human adrenal chromaffin cells through the AT1 receptor
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/5782 https://doi.org/10.1016/s0167-0115(02)00253-7 https://doi.org/10.1016/S0167-0115(02)00253-7 |
Resumo: | The aim of the present work was to study the effect of angiotensin II (Ang II) on catecholamines and neuropeptide Y (NPY) release in primary cultures of human adrenal chromaffin cells. Ang II stimulates norepinephrine (NE), epinephrine (EP) and NPY release from perifused chromaffin cells by 3-, 2- and 12-fold, respectively. The NPY release is more sustained than that of catecholamines. We found that the receptor-AT2 agonist, T2-(Ang II 4-8)2 has no effect on NE, EP and NPY release from chromaffin cells. We further showed that Ang II increases intracellular Ca2+ concentration ([Ca2+]i). The selective AT1-receptor antagonist Candesartan blocked [Ca2+]i increase by Ang II, while T2-(Ang II 4-8)2 was ineffective. These findings demonstrate that AT1 stimulation induces catecholamine secretion from human adrenal chromaffin cells probably by raising cytosolic calcium. |
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Angiotensin II mediates catecholamine and neuropeptide Y secretion in human adrenal chromaffin cells through the AT1 receptorHuman adrenal chromaffin cellsCatecholaminesNeuropeptide YAngiotensin IIAT1 receptorAT2 receptorThe aim of the present work was to study the effect of angiotensin II (Ang II) on catecholamines and neuropeptide Y (NPY) release in primary cultures of human adrenal chromaffin cells. Ang II stimulates norepinephrine (NE), epinephrine (EP) and NPY release from perifused chromaffin cells by 3-, 2- and 12-fold, respectively. The NPY release is more sustained than that of catecholamines. We found that the receptor-AT2 agonist, T2-(Ang II 4-8)2 has no effect on NE, EP and NPY release from chromaffin cells. We further showed that Ang II increases intracellular Ca2+ concentration ([Ca2+]i). The selective AT1-receptor antagonist Candesartan blocked [Ca2+]i increase by Ang II, while T2-(Ang II 4-8)2 was ineffective. These findings demonstrate that AT1 stimulation induces catecholamine secretion from human adrenal chromaffin cells probably by raising cytosolic calcium.http://www.sciencedirect.com/science/article/B6T0S-478J1GP-4/1/703d0edba8be6689cfd19851f6d304e72003info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleaplication/PDFhttp://hdl.handle.net/10316/5782http://hdl.handle.net/10316/5782https://doi.org/10.1016/s0167-0115(02)00253-7https://doi.org/10.1016/S0167-0115(02)00253-7engRegulatory Peptides. 111:1-3 (2003) 61-65Cavadas, CláudiaGrand, DanielaMosimann, FrançoisCotrim, Maria DulceRibeiro, Carlos Alberto FontesBrunner, Hans R.Grouzmann, Ericinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-05-25T02:56:47Zoai:estudogeral.uc.pt:10316/5782Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:47:24.541962Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Angiotensin II mediates catecholamine and neuropeptide Y secretion in human adrenal chromaffin cells through the AT1 receptor |
title |
Angiotensin II mediates catecholamine and neuropeptide Y secretion in human adrenal chromaffin cells through the AT1 receptor |
spellingShingle |
Angiotensin II mediates catecholamine and neuropeptide Y secretion in human adrenal chromaffin cells through the AT1 receptor Cavadas, Cláudia Human adrenal chromaffin cells Catecholamines Neuropeptide Y Angiotensin II AT1 receptor AT2 receptor |
title_short |
Angiotensin II mediates catecholamine and neuropeptide Y secretion in human adrenal chromaffin cells through the AT1 receptor |
title_full |
Angiotensin II mediates catecholamine and neuropeptide Y secretion in human adrenal chromaffin cells through the AT1 receptor |
title_fullStr |
Angiotensin II mediates catecholamine and neuropeptide Y secretion in human adrenal chromaffin cells through the AT1 receptor |
title_full_unstemmed |
Angiotensin II mediates catecholamine and neuropeptide Y secretion in human adrenal chromaffin cells through the AT1 receptor |
title_sort |
Angiotensin II mediates catecholamine and neuropeptide Y secretion in human adrenal chromaffin cells through the AT1 receptor |
author |
Cavadas, Cláudia |
author_facet |
Cavadas, Cláudia Grand, Daniela Mosimann, François Cotrim, Maria Dulce Ribeiro, Carlos Alberto Fontes Brunner, Hans R. Grouzmann, Eric |
author_role |
author |
author2 |
Grand, Daniela Mosimann, François Cotrim, Maria Dulce Ribeiro, Carlos Alberto Fontes Brunner, Hans R. Grouzmann, Eric |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Cavadas, Cláudia Grand, Daniela Mosimann, François Cotrim, Maria Dulce Ribeiro, Carlos Alberto Fontes Brunner, Hans R. Grouzmann, Eric |
dc.subject.por.fl_str_mv |
Human adrenal chromaffin cells Catecholamines Neuropeptide Y Angiotensin II AT1 receptor AT2 receptor |
topic |
Human adrenal chromaffin cells Catecholamines Neuropeptide Y Angiotensin II AT1 receptor AT2 receptor |
description |
The aim of the present work was to study the effect of angiotensin II (Ang II) on catecholamines and neuropeptide Y (NPY) release in primary cultures of human adrenal chromaffin cells. Ang II stimulates norepinephrine (NE), epinephrine (EP) and NPY release from perifused chromaffin cells by 3-, 2- and 12-fold, respectively. The NPY release is more sustained than that of catecholamines. We found that the receptor-AT2 agonist, T2-(Ang II 4-8)2 has no effect on NE, EP and NPY release from chromaffin cells. We further showed that Ang II increases intracellular Ca2+ concentration ([Ca2+]i). The selective AT1-receptor antagonist Candesartan blocked [Ca2+]i increase by Ang II, while T2-(Ang II 4-8)2 was ineffective. These findings demonstrate that AT1 stimulation induces catecholamine secretion from human adrenal chromaffin cells probably by raising cytosolic calcium. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/5782 http://hdl.handle.net/10316/5782 https://doi.org/10.1016/s0167-0115(02)00253-7 https://doi.org/10.1016/S0167-0115(02)00253-7 |
url |
http://hdl.handle.net/10316/5782 https://doi.org/10.1016/s0167-0115(02)00253-7 https://doi.org/10.1016/S0167-0115(02)00253-7 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Regulatory Peptides. 111:1-3 (2003) 61-65 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
aplication/PDF |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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