Effects of sertraline on zebrafish (Danio rerio)

Detalhes bibliográficos
Autor(a) principal: Moreira, Diana Raquel Gomes
Data de Publicação: 2021
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10773/30765
Resumo: The increasing consumption of antidepressants has resulted in its presence in the aquatic environment. This fact has aroused concerns in the scientific community since these biologically active compounds can affect non-target organisms that have physiological systems regulated by these pharmaceuticals. Sertraline (SER) is an antidepressant belonging to the serotonin reuptake inhibitors class (SSRI) and has a high consumption rate. However, there is little knowledge about the toxicological effects of SER in aquatic systems. Thereby, in the Chapter 2 of the present work, a review of the available literature, published in the last five years, addressing levels of antidepressants and its metabolites in rivers, surface waters, tap water, and wastewater treatment plants, as well, the effects reported in fish and invertebrates. Overall, the available laboratory studies show that antidepressants can disrupt multiple biological processes in aquatic organisms, having a detrimental effect. Thus, this study aims to evaluate the short-term (0.1 up to 3000 µg.L-1 ) and long-term (2 up to 1000×103 ng.L-1 ) effects of SER on zebrafish (Danio rerio) assessing different endpoints, such as development (e.g., mortality, spontaneous movements, equilibrium, abnormalities and heartbeat rate), biochemical markers associated with neurotransmission (acetylcholinesterase (AChE)), antioxidant defences (catalase (CAT), glutathione S-tranferases (GSTs)), energy metabolism (lactate dehydrogenase (LDH)) and behaviour (response to light/dark stimulus). Zebrafish embryos demonstrated sensitivity to SER concentrations during development (e.g., decreased spontaneous movements and increased/decreased heartbeat rate, depending on the exposed concentration). Regarding biochemical biomarkers assessed, in short-term exposure GSTs activity was significantly increased, and both in short- and long-term exposures the enzymatic activity of LDH was significantly increased. The responses to light/dark stimulus were generally decreased in short-term exposure, whereas the responses were increased in long-term exposure. These findings support further research on the long-term effects of antidepressants such as SER to aquatic biota and the development new methodologies to efficiently remove them from the environment.
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spelling Effects of sertraline on zebrafish (Danio rerio)Emerging contaminantsAntidepressantsZebrafishDanio rerioShort-term exposureLong-term exposureSertralineDevelopmentBiomarkersBehaviourThe increasing consumption of antidepressants has resulted in its presence in the aquatic environment. This fact has aroused concerns in the scientific community since these biologically active compounds can affect non-target organisms that have physiological systems regulated by these pharmaceuticals. Sertraline (SER) is an antidepressant belonging to the serotonin reuptake inhibitors class (SSRI) and has a high consumption rate. However, there is little knowledge about the toxicological effects of SER in aquatic systems. Thereby, in the Chapter 2 of the present work, a review of the available literature, published in the last five years, addressing levels of antidepressants and its metabolites in rivers, surface waters, tap water, and wastewater treatment plants, as well, the effects reported in fish and invertebrates. Overall, the available laboratory studies show that antidepressants can disrupt multiple biological processes in aquatic organisms, having a detrimental effect. Thus, this study aims to evaluate the short-term (0.1 up to 3000 µg.L-1 ) and long-term (2 up to 1000×103 ng.L-1 ) effects of SER on zebrafish (Danio rerio) assessing different endpoints, such as development (e.g., mortality, spontaneous movements, equilibrium, abnormalities and heartbeat rate), biochemical markers associated with neurotransmission (acetylcholinesterase (AChE)), antioxidant defences (catalase (CAT), glutathione S-tranferases (GSTs)), energy metabolism (lactate dehydrogenase (LDH)) and behaviour (response to light/dark stimulus). Zebrafish embryos demonstrated sensitivity to SER concentrations during development (e.g., decreased spontaneous movements and increased/decreased heartbeat rate, depending on the exposed concentration). Regarding biochemical biomarkers assessed, in short-term exposure GSTs activity was significantly increased, and both in short- and long-term exposures the enzymatic activity of LDH was significantly increased. The responses to light/dark stimulus were generally decreased in short-term exposure, whereas the responses were increased in long-term exposure. These findings support further research on the long-term effects of antidepressants such as SER to aquatic biota and the development new methodologies to efficiently remove them from the environment.O consumo de antidepressivos tem vindo a aumentar, resultando na sua presença no meio aquático. Este facto tem suscitado preocupações na comunidade científica, dado que estes compostos biologicamente ativos podem afetar organismos não-alvo que apresentam sistemas fisiológicos regulados por estes fármacos. A sertralina (SER) é um antidepressivo pertencente à classe dos inibidores seletivos da recaptação de serotonina (SSRI), com uma elevada taxa de utilização. No entanto, apesar do seu elevado consumo, a informação relativa aos seus efeitos tóxicos nos sistemas aquáticos é limitada. Deste modo, no Capítulo 2 deste trabalho, foi feita uma revisão da literatura disponível, publicada nos últimos cinco anos, referindo os níveis de antidepressivos e seus metabolitos no ambiente aquático e, também, os efeitos reportados em peixes e invertebrados. De uma maneira geral, os estudos laboratoriais disponíveis mostraram que os antidepressivos podem alterar vários processos biológicos nos organismos aquáticos, tendo um efeito prejudicial. O presente trabalho tem como objetivo avaliar os efeitos agudos (0.1 a 3000 µg.L-1 ) e crónicos (2 a 1000×103 ng.L-1 ) da SER no peixe-zebra (Danio rerio) avaliando-se diferentes parâmetros, tais como desenvolvimento (como por exemplo, mortalidade, movimentos espontâneos, equilíbrio, anormalidades e batimento cardíaco), biomarcadores bioquímicos associados à neurotransmissão (acetilcolinesterase (AChE)), defesas antioxidantes (catalase (CAT) e glutationa S-transferases (GSTs)), metabolismo energético (lactato desidrogenase (LDH)) e comportamento (resposta a estímulos de luz/escuro). Os embriões de peixezebra demonstraram sensibilidade às concentrações de SER durante o desenvolvimento (por exemplo, diminuição dos movimentos espontâneos e aumento/diminuição dos batimentos cardíacos, dependendo da concentração exposta). Relativamente aos biomarcadores bioquímicos avaliados, na exposição aguda a atividade enzimática das GSTs aumentou significativamente e, tanto na exposição aguda como na exposição crónica, houve um aumento significativo da atividade enzimática da LDH. As respostas aos estímulos de luz/escuro estavam geralmente diminuídas na exposição aguda, enquanto na exposição crónica as respostas estavam elevadas. Estes resultados apoiam a necessidade de se realizarem mais estudos acerca dos efeitos dos antidepressivos a longo prazo, tais como a SER, na biota aquática e o desenvolvimento de novas tecnologias para os remover eficientemente do ambiente.2023-02-16T00:00:00Z2021-02-11T00:00:00Z2021-02-11info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10773/30765engMoreira, Diana Raquel Gomesinfo:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-06T04:30:34Zoai:ria.ua.pt:10773/30765Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-06T04:30:34Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Effects of sertraline on zebrafish (Danio rerio)
title Effects of sertraline on zebrafish (Danio rerio)
spellingShingle Effects of sertraline on zebrafish (Danio rerio)
Moreira, Diana Raquel Gomes
Emerging contaminants
Antidepressants
Zebrafish
Danio rerio
Short-term exposure
Long-term exposure
Sertraline
Development
Biomarkers
Behaviour
title_short Effects of sertraline on zebrafish (Danio rerio)
title_full Effects of sertraline on zebrafish (Danio rerio)
title_fullStr Effects of sertraline on zebrafish (Danio rerio)
title_full_unstemmed Effects of sertraline on zebrafish (Danio rerio)
title_sort Effects of sertraline on zebrafish (Danio rerio)
author Moreira, Diana Raquel Gomes
author_facet Moreira, Diana Raquel Gomes
author_role author
dc.contributor.author.fl_str_mv Moreira, Diana Raquel Gomes
dc.subject.por.fl_str_mv Emerging contaminants
Antidepressants
Zebrafish
Danio rerio
Short-term exposure
Long-term exposure
Sertraline
Development
Biomarkers
Behaviour
topic Emerging contaminants
Antidepressants
Zebrafish
Danio rerio
Short-term exposure
Long-term exposure
Sertraline
Development
Biomarkers
Behaviour
description The increasing consumption of antidepressants has resulted in its presence in the aquatic environment. This fact has aroused concerns in the scientific community since these biologically active compounds can affect non-target organisms that have physiological systems regulated by these pharmaceuticals. Sertraline (SER) is an antidepressant belonging to the serotonin reuptake inhibitors class (SSRI) and has a high consumption rate. However, there is little knowledge about the toxicological effects of SER in aquatic systems. Thereby, in the Chapter 2 of the present work, a review of the available literature, published in the last five years, addressing levels of antidepressants and its metabolites in rivers, surface waters, tap water, and wastewater treatment plants, as well, the effects reported in fish and invertebrates. Overall, the available laboratory studies show that antidepressants can disrupt multiple biological processes in aquatic organisms, having a detrimental effect. Thus, this study aims to evaluate the short-term (0.1 up to 3000 µg.L-1 ) and long-term (2 up to 1000×103 ng.L-1 ) effects of SER on zebrafish (Danio rerio) assessing different endpoints, such as development (e.g., mortality, spontaneous movements, equilibrium, abnormalities and heartbeat rate), biochemical markers associated with neurotransmission (acetylcholinesterase (AChE)), antioxidant defences (catalase (CAT), glutathione S-tranferases (GSTs)), energy metabolism (lactate dehydrogenase (LDH)) and behaviour (response to light/dark stimulus). Zebrafish embryos demonstrated sensitivity to SER concentrations during development (e.g., decreased spontaneous movements and increased/decreased heartbeat rate, depending on the exposed concentration). Regarding biochemical biomarkers assessed, in short-term exposure GSTs activity was significantly increased, and both in short- and long-term exposures the enzymatic activity of LDH was significantly increased. The responses to light/dark stimulus were generally decreased in short-term exposure, whereas the responses were increased in long-term exposure. These findings support further research on the long-term effects of antidepressants such as SER to aquatic biota and the development new methodologies to efficiently remove them from the environment.
publishDate 2021
dc.date.none.fl_str_mv 2021-02-11T00:00:00Z
2021-02-11
2023-02-16T00:00:00Z
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dc.language.iso.fl_str_mv eng
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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