Effects of salinity challenge on the endocrine control of osmoregulation and calcium homeostasis in the sea bream

Detalhes bibliográficos
Autor(a) principal: Guerreiro, P. M.
Data de Publicação: 1999
Outros Autores: Fuentes, J., Flik, G., Canario, Adelino V. M., Power, Deborah
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.1/5497
Resumo: The gilthead sea bream (Sparus auratu) is a marine species often found in coastal lagoons, experimenting episodic exposures to both brackish and hypersaline environments. However, little is known about the underlying endocrine mechanisms controlling osmoregulation in this and in most marine species. This study aimed at characterising some of the endocrine basis of sea bream osmoregulation, with emphasis on calcium homeostasis. Juvenile fish were exposed to different salinities, either by direct transfer or continuous adaptation over a short period of time. Salinities ranged from 0 to 55 p.p.t. and sampling was carried out 4, 24, 96 and 192 h after transfer. Six fish per group and per time point were sacrificed and plasma and tissue samples were collected. Osmolarity, osmolites and cortisol were measured in plasma. Prolactin, growth hormone, stanniocalcin, and calcitonin mRNA expressions were determined by PCR and northern blot. Mortality occurred after 4 hours in FW. Sea bream fry (2 month old, 20-60 may) were exposed to hypersaline and dilute seawater loaded with Ca and calcium fluxes were determined. Exposure of fry to lowered external salinity (50 and 25% SW) resulted in no mortality within 24 h and significantly decreased whole body calcium influx. Results will be discussed in relation to gene expression.
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spelling Effects of salinity challenge on the endocrine control of osmoregulation and calcium homeostasis in the sea breamThe gilthead sea bream (Sparus auratu) is a marine species often found in coastal lagoons, experimenting episodic exposures to both brackish and hypersaline environments. However, little is known about the underlying endocrine mechanisms controlling osmoregulation in this and in most marine species. This study aimed at characterising some of the endocrine basis of sea bream osmoregulation, with emphasis on calcium homeostasis. Juvenile fish were exposed to different salinities, either by direct transfer or continuous adaptation over a short period of time. Salinities ranged from 0 to 55 p.p.t. and sampling was carried out 4, 24, 96 and 192 h after transfer. Six fish per group and per time point were sacrificed and plasma and tissue samples were collected. Osmolarity, osmolites and cortisol were measured in plasma. Prolactin, growth hormone, stanniocalcin, and calcitonin mRNA expressions were determined by PCR and northern blot. Mortality occurred after 4 hours in FW. Sea bream fry (2 month old, 20-60 may) were exposed to hypersaline and dilute seawater loaded with Ca and calcium fluxes were determined. Exposure of fry to lowered external salinity (50 and 25% SW) resulted in no mortality within 24 h and significantly decreased whole body calcium influx. Results will be discussed in relation to gene expression.PMG is in receipt of a PRAXIS XXI grant BD/9207/96T. his study was funded by EC grant FAIR CT-96 1742.ElsevierSapientiaGuerreiro, P. M.Fuentes, J.Flik, G.Canario, Adelino V. M.Power, Deborah2014-10-24T13:59:52Z19991999-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/5497engPM Guerreiro, J Fuentes, G Flik, AVM Canario, DM Power,"Effects of salinity challenge on the endocrine control of osmoregulation and calcium homeostasis in the sea bream" in Comparative Biochemistry and Physiology A-molecular & Integrative Physiology - COMP BIOCHEM PHYSIOL PT A , vol. 124, pp. S80-S80, 1999.1095-6433AUT: PMG01122; DPO00386; ACA00258;http://dx.doi.org/10.1016/S1095-6433(99)90314-8info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:16:38Zoai:sapientia.ualg.pt:10400.1/5497Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:58:28.621115Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Effects of salinity challenge on the endocrine control of osmoregulation and calcium homeostasis in the sea bream
title Effects of salinity challenge on the endocrine control of osmoregulation and calcium homeostasis in the sea bream
spellingShingle Effects of salinity challenge on the endocrine control of osmoregulation and calcium homeostasis in the sea bream
Guerreiro, P. M.
title_short Effects of salinity challenge on the endocrine control of osmoregulation and calcium homeostasis in the sea bream
title_full Effects of salinity challenge on the endocrine control of osmoregulation and calcium homeostasis in the sea bream
title_fullStr Effects of salinity challenge on the endocrine control of osmoregulation and calcium homeostasis in the sea bream
title_full_unstemmed Effects of salinity challenge on the endocrine control of osmoregulation and calcium homeostasis in the sea bream
title_sort Effects of salinity challenge on the endocrine control of osmoregulation and calcium homeostasis in the sea bream
author Guerreiro, P. M.
author_facet Guerreiro, P. M.
Fuentes, J.
Flik, G.
Canario, Adelino V. M.
Power, Deborah
author_role author
author2 Fuentes, J.
Flik, G.
Canario, Adelino V. M.
Power, Deborah
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Sapientia
dc.contributor.author.fl_str_mv Guerreiro, P. M.
Fuentes, J.
Flik, G.
Canario, Adelino V. M.
Power, Deborah
description The gilthead sea bream (Sparus auratu) is a marine species often found in coastal lagoons, experimenting episodic exposures to both brackish and hypersaline environments. However, little is known about the underlying endocrine mechanisms controlling osmoregulation in this and in most marine species. This study aimed at characterising some of the endocrine basis of sea bream osmoregulation, with emphasis on calcium homeostasis. Juvenile fish were exposed to different salinities, either by direct transfer or continuous adaptation over a short period of time. Salinities ranged from 0 to 55 p.p.t. and sampling was carried out 4, 24, 96 and 192 h after transfer. Six fish per group and per time point were sacrificed and plasma and tissue samples were collected. Osmolarity, osmolites and cortisol were measured in plasma. Prolactin, growth hormone, stanniocalcin, and calcitonin mRNA expressions were determined by PCR and northern blot. Mortality occurred after 4 hours in FW. Sea bream fry (2 month old, 20-60 may) were exposed to hypersaline and dilute seawater loaded with Ca and calcium fluxes were determined. Exposure of fry to lowered external salinity (50 and 25% SW) resulted in no mortality within 24 h and significantly decreased whole body calcium influx. Results will be discussed in relation to gene expression.
publishDate 1999
dc.date.none.fl_str_mv 1999
1999-01-01T00:00:00Z
2014-10-24T13:59:52Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.1/5497
url http://hdl.handle.net/10400.1/5497
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv PM Guerreiro, J Fuentes, G Flik, AVM Canario, DM Power,"Effects of salinity challenge on the endocrine control of osmoregulation and calcium homeostasis in the sea bream" in Comparative Biochemistry and Physiology A-molecular & Integrative Physiology - COMP BIOCHEM PHYSIOL PT A , vol. 124, pp. S80-S80, 1999.
1095-6433
AUT: PMG01122; DPO00386; ACA00258;
http://dx.doi.org/10.1016/S1095-6433(99)90314-8
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
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