Exploring silk Sericin for diabetic wounds: an in situ-forming hydrogel to protect against oxidative stress and improve tissue healing and regeneration
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/38363 |
Resumo: | Chronic wounds are one of the most frequent complications that are associated with diabetes mellitus. The overproduction of reactive oxygen species (ROS) is a key factor in the delayed healing of a chronic wound. In the present work, we develop a novel in situ-forming silk sericin-based hydrogel (SSH) that is produced by a simple methodology using horseradish peroxidase (HRP) crosslinking as an advanced dressing for wound healing. The antioxidant and angiogenic effects were assessed in vitro and in vivo after in situ application using an excisional wound-healing model in a genetically-induced diabetic db/db mice and though the chick embryo choriollantoic membrane (CAM) assay, respectively. Wounds in diabetic db/db mice that were treated with SSH closed with reduced granulation tissue, decreased wound edge distance, and wound thickness, when compared to Tegaderm, a dressing that is commonly used in the clinic. The hydrogel also promoted a deposition of collagen fibers with smaller diameter which may have had a boost effect in re-epithelialization. SSH treatment slightly induced two important endogenous antioxidant defenses, superoxide dismutase and catalase. A CAM assay made it possible to observe that SSH led to an increase in the number of newly formed vessels without inducing an inflammatory reaction. The present hydrogel may result in a multi-purpose technology with angiogenic, antioxidant, and anti-inflammatory properties, while advancing efficient and organized tissue regeneration. |
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Exploring silk Sericin for diabetic wounds: an in situ-forming hydrogel to protect against oxidative stress and improve tissue healing and regenerationHorseradish peroxidaseHydrogelIn situ formingSericinSilkWound healingChronic wounds are one of the most frequent complications that are associated with diabetes mellitus. The overproduction of reactive oxygen species (ROS) is a key factor in the delayed healing of a chronic wound. In the present work, we develop a novel in situ-forming silk sericin-based hydrogel (SSH) that is produced by a simple methodology using horseradish peroxidase (HRP) crosslinking as an advanced dressing for wound healing. The antioxidant and angiogenic effects were assessed in vitro and in vivo after in situ application using an excisional wound-healing model in a genetically-induced diabetic db/db mice and though the chick embryo choriollantoic membrane (CAM) assay, respectively. Wounds in diabetic db/db mice that were treated with SSH closed with reduced granulation tissue, decreased wound edge distance, and wound thickness, when compared to Tegaderm, a dressing that is commonly used in the clinic. The hydrogel also promoted a deposition of collagen fibers with smaller diameter which may have had a boost effect in re-epithelialization. SSH treatment slightly induced two important endogenous antioxidant defenses, superoxide dismutase and catalase. A CAM assay made it possible to observe that SSH led to an increase in the number of newly formed vessels without inducing an inflammatory reaction. The present hydrogel may result in a multi-purpose technology with angiogenic, antioxidant, and anti-inflammatory properties, while advancing efficient and organized tissue regeneration.Veritati - Repositório Institucional da Universidade Católica PortuguesaBaptista-Silva, SaraBernardes, Beatriz G.Borges, SandraRodrigues, IldaFernandes, RuiGomes-Guerreiro, SusanaPinto, Marta TeixeiraPintado, ManuelaSoares, RaquelCosta, RaquelOliveira, Ana Leite2022-07-22T16:35:42Z2022-06-082022-06-08T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/38363eng2218-273X10.3390/biom1206080185131410480PMC922129835740928000916417100001info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-16T01:44:23Zoai:repositorio.ucp.pt:10400.14/38363Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:31:19.207782Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Exploring silk Sericin for diabetic wounds: an in situ-forming hydrogel to protect against oxidative stress and improve tissue healing and regeneration |
title |
Exploring silk Sericin for diabetic wounds: an in situ-forming hydrogel to protect against oxidative stress and improve tissue healing and regeneration |
spellingShingle |
Exploring silk Sericin for diabetic wounds: an in situ-forming hydrogel to protect against oxidative stress and improve tissue healing and regeneration Baptista-Silva, Sara Horseradish peroxidase Hydrogel In situ forming Sericin Silk Wound healing |
title_short |
Exploring silk Sericin for diabetic wounds: an in situ-forming hydrogel to protect against oxidative stress and improve tissue healing and regeneration |
title_full |
Exploring silk Sericin for diabetic wounds: an in situ-forming hydrogel to protect against oxidative stress and improve tissue healing and regeneration |
title_fullStr |
Exploring silk Sericin for diabetic wounds: an in situ-forming hydrogel to protect against oxidative stress and improve tissue healing and regeneration |
title_full_unstemmed |
Exploring silk Sericin for diabetic wounds: an in situ-forming hydrogel to protect against oxidative stress and improve tissue healing and regeneration |
title_sort |
Exploring silk Sericin for diabetic wounds: an in situ-forming hydrogel to protect against oxidative stress and improve tissue healing and regeneration |
author |
Baptista-Silva, Sara |
author_facet |
Baptista-Silva, Sara Bernardes, Beatriz G. Borges, Sandra Rodrigues, Ilda Fernandes, Rui Gomes-Guerreiro, Susana Pinto, Marta Teixeira Pintado, Manuela Soares, Raquel Costa, Raquel Oliveira, Ana Leite |
author_role |
author |
author2 |
Bernardes, Beatriz G. Borges, Sandra Rodrigues, Ilda Fernandes, Rui Gomes-Guerreiro, Susana Pinto, Marta Teixeira Pintado, Manuela Soares, Raquel Costa, Raquel Oliveira, Ana Leite |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Baptista-Silva, Sara Bernardes, Beatriz G. Borges, Sandra Rodrigues, Ilda Fernandes, Rui Gomes-Guerreiro, Susana Pinto, Marta Teixeira Pintado, Manuela Soares, Raquel Costa, Raquel Oliveira, Ana Leite |
dc.subject.por.fl_str_mv |
Horseradish peroxidase Hydrogel In situ forming Sericin Silk Wound healing |
topic |
Horseradish peroxidase Hydrogel In situ forming Sericin Silk Wound healing |
description |
Chronic wounds are one of the most frequent complications that are associated with diabetes mellitus. The overproduction of reactive oxygen species (ROS) is a key factor in the delayed healing of a chronic wound. In the present work, we develop a novel in situ-forming silk sericin-based hydrogel (SSH) that is produced by a simple methodology using horseradish peroxidase (HRP) crosslinking as an advanced dressing for wound healing. The antioxidant and angiogenic effects were assessed in vitro and in vivo after in situ application using an excisional wound-healing model in a genetically-induced diabetic db/db mice and though the chick embryo choriollantoic membrane (CAM) assay, respectively. Wounds in diabetic db/db mice that were treated with SSH closed with reduced granulation tissue, decreased wound edge distance, and wound thickness, when compared to Tegaderm, a dressing that is commonly used in the clinic. The hydrogel also promoted a deposition of collagen fibers with smaller diameter which may have had a boost effect in re-epithelialization. SSH treatment slightly induced two important endogenous antioxidant defenses, superoxide dismutase and catalase. A CAM assay made it possible to observe that SSH led to an increase in the number of newly formed vessels without inducing an inflammatory reaction. The present hydrogel may result in a multi-purpose technology with angiogenic, antioxidant, and anti-inflammatory properties, while advancing efficient and organized tissue regeneration. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-07-22T16:35:42Z 2022-06-08 2022-06-08T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/38363 |
url |
http://hdl.handle.net/10400.14/38363 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2218-273X 10.3390/biom12060801 85131410480 PMC9221298 35740928 000916417100001 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132036294770688 |