Quality, Safety and Biological Studies on Campylanthus glaber Aerial Parts
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2023 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/163974 |
Resumo: | Publisher Copyright: © 2023 by the authors. |
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7160 |
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Quality, Safety and Biological Studies on Campylanthus glaber Aerial PartsantihyperglycemicantioxidantCampylanthus glaberherbal drugherbal medicinequality controlsafetyverbascosideMolecular MedicinePharmaceutical ScienceDrug DiscoveryPublisher Copyright: © 2023 by the authors.In Cabo Verde, several endemic species are used in traditional medicine. However, no scientific studies have been conducted on the quality, efficacy, and safety of most of these plants. This study focused on establishing the botanical and chemical identification parameters required for a quality monograph of Campylanthus glaber Benth. aerial parts, a medicinal plant of Cabo Verde traditionally used to treat fever and muscular pain. In addition, in vitro antioxidant and antihyperglycemic activity, cytotoxicity, and genotoxicity were assessed for this medicinal plant. Optical microscopy, LC/UV-DAD-ESI/MS, and colorimetric assays were used for botanical, chemical, and biological studies, respectively. Cytotoxicity was assessed by the MTT assay with HepG2 cells, and genotoxicity by the Ames test. Microscopically, the xeromorphic leaf of C. glaber presents a thick cuticle (13.6–25.5 µm), thick-walled epidermal cells, anomocytic-type stomata, glandular trichomes (stalk length = 49.4–120.8 µm), and idioblasts containing calcium oxalate microcrystals. The chemical screening of aqueous and hydroethanolic extracts of this medicinal plant revealed the presence of organic acids, iridoids, phenylethanoids, and flavonoids as the main classes of marker compounds, with malic acid, citric acid, and verbascoside being the main marker compounds identified. Both extracts showed similar LC/UV-DAD/ESI-MS qualitative profiles and DPPH radical scavenger activity (IC50 = 130.9 ± 1.4; 134.3 ± 3.1 µg/mL). The hydroethanolic extract inhibited both α-amylase and α-glucosidase enzymes in a dose-dependent manner. Both extracts showed no cytotoxicity (up to 1000 µg/mL) by the MTT assay and no genotoxic potential with or without metabolic activation up to 5 mg /plate. The results obtained are an important contribution to the monographic quality assessment of C. glaber aerial parts and suggest that this medicinal plant may be safe and potentially used as an herbal drug raw material for pharmaceutical purposes.MEtRICS - Centro de Engenharia Mecânica e Sustentabilidade de RecursosRUNLima, KateleneMalmir, MaryamCamões, Sérgio P.Hasan, KamrulGomes, SamuelMoreira da Silva, IsabelFigueira, Maria EduardoMiranda, Joana P.Serrano, RitaDuarte, Maria PaulaSilva, Olga2024-02-22T23:52:08Z2023-09-282023-09-28T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article24application/pdfhttp://hdl.handle.net/10362/163974eng1424-8247PURE: 83888465https://doi.org/10.3390/ph16101373info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:49:58Zoai:run.unl.pt:10362/163974Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:59:58.056368Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Quality, Safety and Biological Studies on Campylanthus glaber Aerial Parts |
| title |
Quality, Safety and Biological Studies on Campylanthus glaber Aerial Parts |
| spellingShingle |
Quality, Safety and Biological Studies on Campylanthus glaber Aerial Parts Lima, Katelene antihyperglycemic antioxidant Campylanthus glaber herbal drug herbal medicine quality control safety verbascoside Molecular Medicine Pharmaceutical Science Drug Discovery |
| title_short |
Quality, Safety and Biological Studies on Campylanthus glaber Aerial Parts |
| title_full |
Quality, Safety and Biological Studies on Campylanthus glaber Aerial Parts |
| title_fullStr |
Quality, Safety and Biological Studies on Campylanthus glaber Aerial Parts |
| title_full_unstemmed |
Quality, Safety and Biological Studies on Campylanthus glaber Aerial Parts |
| title_sort |
Quality, Safety and Biological Studies on Campylanthus glaber Aerial Parts |
| author |
Lima, Katelene |
| author_facet |
Lima, Katelene Malmir, Maryam Camões, Sérgio P. Hasan, Kamrul Gomes, Samuel Moreira da Silva, Isabel Figueira, Maria Eduardo Miranda, Joana P. Serrano, Rita Duarte, Maria Paula Silva, Olga |
| author_role |
author |
| author2 |
Malmir, Maryam Camões, Sérgio P. Hasan, Kamrul Gomes, Samuel Moreira da Silva, Isabel Figueira, Maria Eduardo Miranda, Joana P. Serrano, Rita Duarte, Maria Paula Silva, Olga |
| author2_role |
author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
MEtRICS - Centro de Engenharia Mecânica e Sustentabilidade de Recursos RUN |
| dc.contributor.author.fl_str_mv |
Lima, Katelene Malmir, Maryam Camões, Sérgio P. Hasan, Kamrul Gomes, Samuel Moreira da Silva, Isabel Figueira, Maria Eduardo Miranda, Joana P. Serrano, Rita Duarte, Maria Paula Silva, Olga |
| dc.subject.por.fl_str_mv |
antihyperglycemic antioxidant Campylanthus glaber herbal drug herbal medicine quality control safety verbascoside Molecular Medicine Pharmaceutical Science Drug Discovery |
| topic |
antihyperglycemic antioxidant Campylanthus glaber herbal drug herbal medicine quality control safety verbascoside Molecular Medicine Pharmaceutical Science Drug Discovery |
| description |
Publisher Copyright: © 2023 by the authors. |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2023-09-28 2023-09-28T00:00:00Z 2024-02-22T23:52:08Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/163974 |
| url |
http://hdl.handle.net/10362/163974 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
1424-8247 PURE: 83888465 https://doi.org/10.3390/ph16101373 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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24 application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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