Enzymatic degradation of starch-based thermoplastic compounds used in protheses : identification of the degradation products in solution
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/1413 |
Resumo: | Apart from favourable physico-chemical and mechanical properties, the most important requirement for a biodegradable polymer to be used in medical applications is its biocompatibility and the non-cytotoxicity of its degradation products. Their combined effect should assure the safe material degradation under controlled kinetics. The present work analyses the degradation behaviour of blends of corn starch with poly(ethylene-vinyl alcohol) copolymer (SEVA-C). The characterization included long-term degradation trials on simulated physiological solution with a-amylase up to 200 days. The degradation solutions were analysed by several techniques. High-performance liquid chromatography (HPLC) and colorimetric methods were used to monitor the liberation of carbohydrate as a consequence of starch hydrolysis by a-amylase. The hydration degree was followed by thermogravimetric analysis (TGA). Several degradation products such as carbohydrates ranging from C6 to C18 were identified. After a-amylase action, biodegradation was more pronounced in the first 100 days, after which the biodegradation rate decreased probably due to the structure and porosity of the material. The action of a-amylase solely led to the starch degradation, in contrast with other assays without enzymes where no carbohydrates were found in the degradation solutions. |
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Enzymatic degradation of starch-based thermoplastic compounds used in protheses : identification of the degradation products in solutionBiodegradable polymerIn vitro testStarchEnzymePolysaccharidePolyvinylalcoholScience & TechnologyApart from favourable physico-chemical and mechanical properties, the most important requirement for a biodegradable polymer to be used in medical applications is its biocompatibility and the non-cytotoxicity of its degradation products. Their combined effect should assure the safe material degradation under controlled kinetics. The present work analyses the degradation behaviour of blends of corn starch with poly(ethylene-vinyl alcohol) copolymer (SEVA-C). The characterization included long-term degradation trials on simulated physiological solution with a-amylase up to 200 days. The degradation solutions were analysed by several techniques. High-performance liquid chromatography (HPLC) and colorimetric methods were used to monitor the liberation of carbohydrate as a consequence of starch hydrolysis by a-amylase. The hydration degree was followed by thermogravimetric analysis (TGA). Several degradation products such as carbohydrates ranging from C6 to C18 were identified. After a-amylase action, biodegradation was more pronounced in the first 100 days, after which the biodegradation rate decreased probably due to the structure and porosity of the material. The action of a-amylase solely led to the starch degradation, in contrast with other assays without enzymes where no carbohydrates were found in the degradation solutions.Elsevier ScienceUniversidade do MinhoAraújo, M. AlbertaCunha, A. M.Mota, M.20042004-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/1413eng“Biomaterials”. 25:13 (2004) 2687-2693.0142-961210.1016/j.biomaterials.2003.09.09314751755info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:52:42Zoai:repositorium.sdum.uminho.pt:1822/1413Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:51:53.483860Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Enzymatic degradation of starch-based thermoplastic compounds used in protheses : identification of the degradation products in solution |
title |
Enzymatic degradation of starch-based thermoplastic compounds used in protheses : identification of the degradation products in solution |
spellingShingle |
Enzymatic degradation of starch-based thermoplastic compounds used in protheses : identification of the degradation products in solution Araújo, M. Alberta Biodegradable polymer In vitro test Starch Enzyme Polysaccharide Polyvinylalcohol Science & Technology |
title_short |
Enzymatic degradation of starch-based thermoplastic compounds used in protheses : identification of the degradation products in solution |
title_full |
Enzymatic degradation of starch-based thermoplastic compounds used in protheses : identification of the degradation products in solution |
title_fullStr |
Enzymatic degradation of starch-based thermoplastic compounds used in protheses : identification of the degradation products in solution |
title_full_unstemmed |
Enzymatic degradation of starch-based thermoplastic compounds used in protheses : identification of the degradation products in solution |
title_sort |
Enzymatic degradation of starch-based thermoplastic compounds used in protheses : identification of the degradation products in solution |
author |
Araújo, M. Alberta |
author_facet |
Araújo, M. Alberta Cunha, A. M. Mota, M. |
author_role |
author |
author2 |
Cunha, A. M. Mota, M. |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Araújo, M. Alberta Cunha, A. M. Mota, M. |
dc.subject.por.fl_str_mv |
Biodegradable polymer In vitro test Starch Enzyme Polysaccharide Polyvinylalcohol Science & Technology |
topic |
Biodegradable polymer In vitro test Starch Enzyme Polysaccharide Polyvinylalcohol Science & Technology |
description |
Apart from favourable physico-chemical and mechanical properties, the most important requirement for a biodegradable polymer to be used in medical applications is its biocompatibility and the non-cytotoxicity of its degradation products. Their combined effect should assure the safe material degradation under controlled kinetics. The present work analyses the degradation behaviour of blends of corn starch with poly(ethylene-vinyl alcohol) copolymer (SEVA-C). The characterization included long-term degradation trials on simulated physiological solution with a-amylase up to 200 days. The degradation solutions were analysed by several techniques. High-performance liquid chromatography (HPLC) and colorimetric methods were used to monitor the liberation of carbohydrate as a consequence of starch hydrolysis by a-amylase. The hydration degree was followed by thermogravimetric analysis (TGA). Several degradation products such as carbohydrates ranging from C6 to C18 were identified. After a-amylase action, biodegradation was more pronounced in the first 100 days, after which the biodegradation rate decreased probably due to the structure and porosity of the material. The action of a-amylase solely led to the starch degradation, in contrast with other assays without enzymes where no carbohydrates were found in the degradation solutions. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004 2004-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/1413 |
url |
http://hdl.handle.net/1822/1413 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
“Biomaterials”. 25:13 (2004) 2687-2693. 0142-9612 10.1016/j.biomaterials.2003.09.093 14751755 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Science |
publisher.none.fl_str_mv |
Elsevier Science |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133108854849536 |