In vitro activities of Ceiba speciosa (A.St.-Hil) Ravenna aqueous stem bark extract

• Autor(a) principal: Dörr, Juliana Andréa
• Data de Publicação: 2018
• Outros Autores: Bitencourt, Shanna, Bortoluzzi, Luísa, Alves, Celso, Silva, Joana, Boligon, Aline Augusti, Santos, Roberto Christ Vianna, Laufer, Stefan, Pedrosa, Rui, Goettert, Márcia Inês
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10400.8/6144
• topic: Ceiba speciosa; Malvaceae; Polyphenols; Antioxidant; Mitochondrial; Depolarization; Caspase-9

Several species of the genus Ceiba (Malvaceae) are ethnopharmacologically used. Thus, this study aimed to investigate the in vitro beneficial properties of the aqueous stem bark extract of Ceiba speciosa. The extract presented a great amount of phenolic compounds (117.4 ± 6.2 mg GAE/g). The antioxidant activity was assessed by DPPH (IC50 = 42.87 μg/mL), ORAC (2351.17 μmol TE/g) and FRAP (235.94 μM FeSO4/g) methods. In addition, the extract reduced MCF-7 cell viability as assessed by MTT. However, it prevented mitochondrial membrane depolarization and reduced caspase-9 activity induced by hydrogen peroxide. In conclusion, these findings indicate the extract is an excellent source of natural antioxidants and is able to protect ROS-induced cell death. Therefore, C. speciosa extract may possess beneficial properties for application in pharmaceutical industry as an antioxidant. However, further studies to better elucidate its mechanisms and to isolate its active compounds are required.