Process development for the production of prebiotic fructo-oligosaccharides by Penicillium citreonigrum

• Autor(a) principal: Gonçalves, Clarisse Salomé Nobre
• Data de Publicação: 2019
• Outros Autores: Nascimento, A. K. C., Silva, Soraia Pires, Coelho, Elisabete, Coimbra, Manuel A., Cavalcanti, Maria Taciana Holanda, Teixeira, J. A., Porto, Ana Lúcia Figueiredo
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/1822/59644
• topic: Fructo-oligosaccharides; Penicillium citreonigrum; Production yield optimization; prebiotic evaluation; Glycosidic linkage analysis; In vitro prebiotic evaluation; Ciências Médicas::Biotecnologia Médica; Science & Technology

A new isolated P. citreonigrum URM 4459 was selected to produce fructooligosaccharides (FOS) in an efficient, economical and fast one-step fermentation. Optimal culture conditions were stablished by experimental design. Experiments run in bioreactor resulted in a high yield, content, productivity and purity of FOS (0.65 ± 0.06 gFOS.ginitial sucrose-1, 126.3 ± 0.1 g/L, 2.28 ± 0.08 g/L.h and 61 ± 0 %). The FOS mixture was purified up to 92 % (w/w) with an activated charcoal column. FOS produced were able to promote lactobacilli and bifidobacteria growth. Higher bacteria cell density was obtained for microbial-FOS mixtures than commercial Raftilose® P95. Some strains grew even faster in the FOS mixture produced than in all other carbon sources. FOS were resistant to the simulated gastrointestinal conditions. A high amount of a reducing trisaccharide was identified in the FOS produced mixture, possibly neokestose, which may explain the great prebiotic potential of the FOS.