Evidence for TTAGG telomere repeats and rRNA gene clusters in leafhoppers of the genus Alebra (Hemiptera: Auchenorrhyncha: Cicadellidae)
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.13/3961 |
Resumo: | The leafhopper genus Alebra Fieber, 1872 comprises a complex of morphologically similar species. The chromosome complements (karyotypes) of five Alebra species, i.e. A. albostriella, A. coryli, A. viridis, A. wahlbergi and a new, yet undescribed species, provisionally named Taxon 1, were here investigated, three of these species (A. coryli, A. viridis, and Taxon 1) for the first time. The techniques applied included standard chromosome staining, fluorescence in situ hybridization (FISH) for mapping of 18S rDNA and telomeric repeats (in every species), C-banding, AgNOR-banding and CMA3 /DAPI- staining (in A. viridis). The species have a holokinetic type of chromosomes, as in other hemipterans. Karyotypes of all species are remarkably conserved with 2n = 22 + X(0)/XX (male/female), one large and 10 medium pairs of autosomes and the X chromosome similar in size to larger chromosomes within this group. In every species, FISH identified the “classical” insect telomere repeat of TTAGG and rRNA gene clusters located on the homologues of a medium-sized pair of autosomes, presumably number 5. Thus, speciation in Alebra has apparently not involved significant karyotypic changes. In A. viridis, rDNA sites were both Ag- and CMA3 -positive and were located at an interstitial position. C-banding revealed heterochromatic bands in the X chromosome and also in all but four pairs of autosomes, the bands were located at one telomere of a chromosome. C-bands were positive for CMA3 and negative for DAPI, suggesting that C-heterochromatin is mainly enriched in GC-pairs. |
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Evidence for TTAGG telomere repeats and rRNA gene clusters in leafhoppers of the genus Alebra (Hemiptera: Auchenorrhyncha: Cicadellidae)HemipteraCicadellidaeAlebraKaryotypeHolokinetic chromosomesC-heterochromatinCMA3 /DAPIAg-NORFISH18S rDNATTAGG.Faculdade de Ciências da VidaThe leafhopper genus Alebra Fieber, 1872 comprises a complex of morphologically similar species. The chromosome complements (karyotypes) of five Alebra species, i.e. A. albostriella, A. coryli, A. viridis, A. wahlbergi and a new, yet undescribed species, provisionally named Taxon 1, were here investigated, three of these species (A. coryli, A. viridis, and Taxon 1) for the first time. The techniques applied included standard chromosome staining, fluorescence in situ hybridization (FISH) for mapping of 18S rDNA and telomeric repeats (in every species), C-banding, AgNOR-banding and CMA3 /DAPI- staining (in A. viridis). The species have a holokinetic type of chromosomes, as in other hemipterans. Karyotypes of all species are remarkably conserved with 2n = 22 + X(0)/XX (male/female), one large and 10 medium pairs of autosomes and the X chromosome similar in size to larger chromosomes within this group. In every species, FISH identified the “classical” insect telomere repeat of TTAGG and rRNA gene clusters located on the homologues of a medium-sized pair of autosomes, presumably number 5. Thus, speciation in Alebra has apparently not involved significant karyotypic changes. In A. viridis, rDNA sites were both Ag- and CMA3 -positive and were located at an interstitial position. C-banding revealed heterochromatic bands in the X chromosome and also in all but four pairs of autosomes, the bands were located at one telomere of a chromosome. C-bands were positive for CMA3 and negative for DAPI, suggesting that C-heterochromatin is mainly enriched in GC-pairs.Institute of EntomologyDigitUMaKuzNnetsova, Valentina G.Maryanska-Nadachowska, AnnaAnokhin, BorisAguin-Pombo, Dora2022-01-07T12:04:08Z2015-01-01T00:00:00Z2015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.13/3961engKuznetsova, V. G., Maryańska-Nadachowska, A. N. N. A., Anokhin, B., & Aguin-Pombo, D. (2015). Evidence for TTAGG telomere repeats and rRNA gene clusters in leafhoppers of the genus Alebra (Hemiptera: Auchenorrhyncha: Cicadellidae). European Journal of Entomology, 112(2). doi: 10.14411/eje.2015.04510.14411/eje.2015.045info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-09-05T12:57:04Zoai:digituma.uma.pt:10400.13/3961Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:07:32.320548Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Evidence for TTAGG telomere repeats and rRNA gene clusters in leafhoppers of the genus Alebra (Hemiptera: Auchenorrhyncha: Cicadellidae) |
title |
Evidence for TTAGG telomere repeats and rRNA gene clusters in leafhoppers of the genus Alebra (Hemiptera: Auchenorrhyncha: Cicadellidae) |
spellingShingle |
Evidence for TTAGG telomere repeats and rRNA gene clusters in leafhoppers of the genus Alebra (Hemiptera: Auchenorrhyncha: Cicadellidae) KuzNnetsova, Valentina G. Hemiptera Cicadellidae Alebra Karyotype Holokinetic chromosomes C-heterochromatin CMA3 /DAPI Ag-NOR FISH 18S rDNA TTAGG . Faculdade de Ciências da Vida |
title_short |
Evidence for TTAGG telomere repeats and rRNA gene clusters in leafhoppers of the genus Alebra (Hemiptera: Auchenorrhyncha: Cicadellidae) |
title_full |
Evidence for TTAGG telomere repeats and rRNA gene clusters in leafhoppers of the genus Alebra (Hemiptera: Auchenorrhyncha: Cicadellidae) |
title_fullStr |
Evidence for TTAGG telomere repeats and rRNA gene clusters in leafhoppers of the genus Alebra (Hemiptera: Auchenorrhyncha: Cicadellidae) |
title_full_unstemmed |
Evidence for TTAGG telomere repeats and rRNA gene clusters in leafhoppers of the genus Alebra (Hemiptera: Auchenorrhyncha: Cicadellidae) |
title_sort |
Evidence for TTAGG telomere repeats and rRNA gene clusters in leafhoppers of the genus Alebra (Hemiptera: Auchenorrhyncha: Cicadellidae) |
author |
KuzNnetsova, Valentina G. |
author_facet |
KuzNnetsova, Valentina G. Maryanska-Nadachowska, Anna Anokhin, Boris Aguin-Pombo, Dora |
author_role |
author |
author2 |
Maryanska-Nadachowska, Anna Anokhin, Boris Aguin-Pombo, Dora |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
DigitUMa |
dc.contributor.author.fl_str_mv |
KuzNnetsova, Valentina G. Maryanska-Nadachowska, Anna Anokhin, Boris Aguin-Pombo, Dora |
dc.subject.por.fl_str_mv |
Hemiptera Cicadellidae Alebra Karyotype Holokinetic chromosomes C-heterochromatin CMA3 /DAPI Ag-NOR FISH 18S rDNA TTAGG . Faculdade de Ciências da Vida |
topic |
Hemiptera Cicadellidae Alebra Karyotype Holokinetic chromosomes C-heterochromatin CMA3 /DAPI Ag-NOR FISH 18S rDNA TTAGG . Faculdade de Ciências da Vida |
description |
The leafhopper genus Alebra Fieber, 1872 comprises a complex of morphologically similar species. The chromosome complements (karyotypes) of five Alebra species, i.e. A. albostriella, A. coryli, A. viridis, A. wahlbergi and a new, yet undescribed species, provisionally named Taxon 1, were here investigated, three of these species (A. coryli, A. viridis, and Taxon 1) for the first time. The techniques applied included standard chromosome staining, fluorescence in situ hybridization (FISH) for mapping of 18S rDNA and telomeric repeats (in every species), C-banding, AgNOR-banding and CMA3 /DAPI- staining (in A. viridis). The species have a holokinetic type of chromosomes, as in other hemipterans. Karyotypes of all species are remarkably conserved with 2n = 22 + X(0)/XX (male/female), one large and 10 medium pairs of autosomes and the X chromosome similar in size to larger chromosomes within this group. In every species, FISH identified the “classical” insect telomere repeat of TTAGG and rRNA gene clusters located on the homologues of a medium-sized pair of autosomes, presumably number 5. Thus, speciation in Alebra has apparently not involved significant karyotypic changes. In A. viridis, rDNA sites were both Ag- and CMA3 -positive and were located at an interstitial position. C-banding revealed heterochromatic bands in the X chromosome and also in all but four pairs of autosomes, the bands were located at one telomere of a chromosome. C-bands were positive for CMA3 and negative for DAPI, suggesting that C-heterochromatin is mainly enriched in GC-pairs. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-01-01T00:00:00Z 2015-01-01T00:00:00Z 2022-01-07T12:04:08Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.13/3961 |
url |
http://hdl.handle.net/10400.13/3961 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Kuznetsova, V. G., Maryańska-Nadachowska, A. N. N. A., Anokhin, B., & Aguin-Pombo, D. (2015). Evidence for TTAGG telomere repeats and rRNA gene clusters in leafhoppers of the genus Alebra (Hemiptera: Auchenorrhyncha: Cicadellidae). European Journal of Entomology, 112(2). doi: 10.14411/eje.2015.045 10.14411/eje.2015.045 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Institute of Entomology |
publisher.none.fl_str_mv |
Institute of Entomology |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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