Guidelines for the Direct Detection of Anaplasma spp. in Diagnosis and Epidemiological Studies

Detalhes bibliográficos
Autor(a) principal: Silaghi, C.
Data de Publicação: 2017
Outros Autores: Santos, A.S., Gomes, J., Christova, I., Matei, I.A., Walder, G., Domingos, A., Bell-Sakyi, L., Sprong, H., von Loewenich, F.D., Oteo, J.A., de la Fuente, J., Dumler, J.S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/4902
Resumo: The genus Anaplasma (Rickettsiales: Anaplasmataceae) comprises obligate intracellular Gram-negative bacteria that are mainly transmitted by ticks, and currently includes six species: Anaplasma bovis, Anaplasma centrale, Anaplasma marginale, Anaplasma phagocytophilum, Anaplasma platys, and Anaplasma ovis. These have long been known as etiological agents of veterinary diseases that affect domestic and wild animals worldwide. A zoonotic role has been recognized for A. phagocytophilum, but other species can also be pathogenic for humans. Anaplasma infections are usually challenging to diagnose, clinically presenting with nonspecific symptoms that vary greatly depending on the agent involved, the affected host, and other factors such as immune status and coinfections. The substantial economic impact associated with livestock infection and the growing number of human cases along with the risk of transfusion-transmitted infections, determines the need for accurate laboratory tests. Because hosts are usually seronegative in the initial phase of infection and serological cross-reactions with several Anaplasma species are observed after seroconversion, direct tests are the best approach for both case definition and epidemiological studies. Blood samples are routinely used for Anaplasma spp. screening, but in persistently infected animals with intermittent or low-level bacteremia, other tissues might be useful. These guidelines have been developed as a direct outcome of the COST action TD1303 EURNEGVEC ("European Network of Neglected Vectors and Vector-Borne Diseases"). They review the direct laboratory tests (microscopy, nucleic acid-based detection and in vitro isolation) currently used for Anaplasma detection in ticks and vertebrates and their application.
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spelling Guidelines for the Direct Detection of Anaplasma spp. in Diagnosis and Epidemiological StudiesAnaplasma spp.PCRDirect DiagnosisIn vitro IsolationMicroscopyTicksVertebrate HostsInfecções Sistémicas e ZoonosesThe genus Anaplasma (Rickettsiales: Anaplasmataceae) comprises obligate intracellular Gram-negative bacteria that are mainly transmitted by ticks, and currently includes six species: Anaplasma bovis, Anaplasma centrale, Anaplasma marginale, Anaplasma phagocytophilum, Anaplasma platys, and Anaplasma ovis. These have long been known as etiological agents of veterinary diseases that affect domestic and wild animals worldwide. A zoonotic role has been recognized for A. phagocytophilum, but other species can also be pathogenic for humans. Anaplasma infections are usually challenging to diagnose, clinically presenting with nonspecific symptoms that vary greatly depending on the agent involved, the affected host, and other factors such as immune status and coinfections. The substantial economic impact associated with livestock infection and the growing number of human cases along with the risk of transfusion-transmitted infections, determines the need for accurate laboratory tests. Because hosts are usually seronegative in the initial phase of infection and serological cross-reactions with several Anaplasma species are observed after seroconversion, direct tests are the best approach for both case definition and epidemiological studies. Blood samples are routinely used for Anaplasma spp. screening, but in persistently infected animals with intermittent or low-level bacteremia, other tissues might be useful. These guidelines have been developed as a direct outcome of the COST action TD1303 EURNEGVEC ("European Network of Neglected Vectors and Vector-Borne Diseases"). They review the direct laboratory tests (microscopy, nucleic acid-based detection and in vitro isolation) currently used for Anaplasma detection in ticks and vertebrates and their application.Mary Ann Liebert/Society for Zoonotic Ecology and Epidemiology (SocZEE)Repositório Científico do Instituto Nacional de SaúdeSilaghi, C.Santos, A.S.Gomes, J.Christova, I.Matei, I.A.Walder, G.Domingos, A.Bell-Sakyi, L.Sprong, H.von Loewenich, F.D.Oteo, J.A.de la Fuente, J.Dumler, J.S.2018-02-01T15:42:11Z2017-012017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/4902engVector Borne Zoonotic Dis. 2017 Jan;17(1):12-22.1530-366710.1089/vbz.2016.1960info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:40:22Zoai:repositorio.insa.pt:10400.18/4902Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:39:17.087409Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Guidelines for the Direct Detection of Anaplasma spp. in Diagnosis and Epidemiological Studies
title Guidelines for the Direct Detection of Anaplasma spp. in Diagnosis and Epidemiological Studies
spellingShingle Guidelines for the Direct Detection of Anaplasma spp. in Diagnosis and Epidemiological Studies
Silaghi, C.
Anaplasma spp.
PCR
Direct Diagnosis
In vitro Isolation
Microscopy
Ticks
Vertebrate Hosts
Infecções Sistémicas e Zoonoses
title_short Guidelines for the Direct Detection of Anaplasma spp. in Diagnosis and Epidemiological Studies
title_full Guidelines for the Direct Detection of Anaplasma spp. in Diagnosis and Epidemiological Studies
title_fullStr Guidelines for the Direct Detection of Anaplasma spp. in Diagnosis and Epidemiological Studies
title_full_unstemmed Guidelines for the Direct Detection of Anaplasma spp. in Diagnosis and Epidemiological Studies
title_sort Guidelines for the Direct Detection of Anaplasma spp. in Diagnosis and Epidemiological Studies
author Silaghi, C.
author_facet Silaghi, C.
Santos, A.S.
Gomes, J.
Christova, I.
Matei, I.A.
Walder, G.
Domingos, A.
Bell-Sakyi, L.
Sprong, H.
von Loewenich, F.D.
Oteo, J.A.
de la Fuente, J.
Dumler, J.S.
author_role author
author2 Santos, A.S.
Gomes, J.
Christova, I.
Matei, I.A.
Walder, G.
Domingos, A.
Bell-Sakyi, L.
Sprong, H.
von Loewenich, F.D.
Oteo, J.A.
de la Fuente, J.
Dumler, J.S.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Silaghi, C.
Santos, A.S.
Gomes, J.
Christova, I.
Matei, I.A.
Walder, G.
Domingos, A.
Bell-Sakyi, L.
Sprong, H.
von Loewenich, F.D.
Oteo, J.A.
de la Fuente, J.
Dumler, J.S.
dc.subject.por.fl_str_mv Anaplasma spp.
PCR
Direct Diagnosis
In vitro Isolation
Microscopy
Ticks
Vertebrate Hosts
Infecções Sistémicas e Zoonoses
topic Anaplasma spp.
PCR
Direct Diagnosis
In vitro Isolation
Microscopy
Ticks
Vertebrate Hosts
Infecções Sistémicas e Zoonoses
description The genus Anaplasma (Rickettsiales: Anaplasmataceae) comprises obligate intracellular Gram-negative bacteria that are mainly transmitted by ticks, and currently includes six species: Anaplasma bovis, Anaplasma centrale, Anaplasma marginale, Anaplasma phagocytophilum, Anaplasma platys, and Anaplasma ovis. These have long been known as etiological agents of veterinary diseases that affect domestic and wild animals worldwide. A zoonotic role has been recognized for A. phagocytophilum, but other species can also be pathogenic for humans. Anaplasma infections are usually challenging to diagnose, clinically presenting with nonspecific symptoms that vary greatly depending on the agent involved, the affected host, and other factors such as immune status and coinfections. The substantial economic impact associated with livestock infection and the growing number of human cases along with the risk of transfusion-transmitted infections, determines the need for accurate laboratory tests. Because hosts are usually seronegative in the initial phase of infection and serological cross-reactions with several Anaplasma species are observed after seroconversion, direct tests are the best approach for both case definition and epidemiological studies. Blood samples are routinely used for Anaplasma spp. screening, but in persistently infected animals with intermittent or low-level bacteremia, other tissues might be useful. These guidelines have been developed as a direct outcome of the COST action TD1303 EURNEGVEC ("European Network of Neglected Vectors and Vector-Borne Diseases"). They review the direct laboratory tests (microscopy, nucleic acid-based detection and in vitro isolation) currently used for Anaplasma detection in ticks and vertebrates and their application.
publishDate 2017
dc.date.none.fl_str_mv 2017-01
2017-01-01T00:00:00Z
2018-02-01T15:42:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/4902
url http://hdl.handle.net/10400.18/4902
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Vector Borne Zoonotic Dis. 2017 Jan;17(1):12-22.
1530-3667
10.1089/vbz.2016.1960
dc.rights.driver.fl_str_mv info:eu-repo/semantics/embargoedAccess
eu_rights_str_mv embargoedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Mary Ann Liebert/Society for Zoonotic Ecology and Epidemiology (SocZEE)
publisher.none.fl_str_mv Mary Ann Liebert/Society for Zoonotic Ecology and Epidemiology (SocZEE)
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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