Guidelines for the Direct Detection of Anaplasma spp. in Diagnosis and Epidemiological Studies
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.18/4902 |
Resumo: | The genus Anaplasma (Rickettsiales: Anaplasmataceae) comprises obligate intracellular Gram-negative bacteria that are mainly transmitted by ticks, and currently includes six species: Anaplasma bovis, Anaplasma centrale, Anaplasma marginale, Anaplasma phagocytophilum, Anaplasma platys, and Anaplasma ovis. These have long been known as etiological agents of veterinary diseases that affect domestic and wild animals worldwide. A zoonotic role has been recognized for A. phagocytophilum, but other species can also be pathogenic for humans. Anaplasma infections are usually challenging to diagnose, clinically presenting with nonspecific symptoms that vary greatly depending on the agent involved, the affected host, and other factors such as immune status and coinfections. The substantial economic impact associated with livestock infection and the growing number of human cases along with the risk of transfusion-transmitted infections, determines the need for accurate laboratory tests. Because hosts are usually seronegative in the initial phase of infection and serological cross-reactions with several Anaplasma species are observed after seroconversion, direct tests are the best approach for both case definition and epidemiological studies. Blood samples are routinely used for Anaplasma spp. screening, but in persistently infected animals with intermittent or low-level bacteremia, other tissues might be useful. These guidelines have been developed as a direct outcome of the COST action TD1303 EURNEGVEC ("European Network of Neglected Vectors and Vector-Borne Diseases"). They review the direct laboratory tests (microscopy, nucleic acid-based detection and in vitro isolation) currently used for Anaplasma detection in ticks and vertebrates and their application. |
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Guidelines for the Direct Detection of Anaplasma spp. in Diagnosis and Epidemiological StudiesAnaplasma spp.PCRDirect DiagnosisIn vitro IsolationMicroscopyTicksVertebrate HostsInfecções Sistémicas e ZoonosesThe genus Anaplasma (Rickettsiales: Anaplasmataceae) comprises obligate intracellular Gram-negative bacteria that are mainly transmitted by ticks, and currently includes six species: Anaplasma bovis, Anaplasma centrale, Anaplasma marginale, Anaplasma phagocytophilum, Anaplasma platys, and Anaplasma ovis. These have long been known as etiological agents of veterinary diseases that affect domestic and wild animals worldwide. A zoonotic role has been recognized for A. phagocytophilum, but other species can also be pathogenic for humans. Anaplasma infections are usually challenging to diagnose, clinically presenting with nonspecific symptoms that vary greatly depending on the agent involved, the affected host, and other factors such as immune status and coinfections. The substantial economic impact associated with livestock infection and the growing number of human cases along with the risk of transfusion-transmitted infections, determines the need for accurate laboratory tests. Because hosts are usually seronegative in the initial phase of infection and serological cross-reactions with several Anaplasma species are observed after seroconversion, direct tests are the best approach for both case definition and epidemiological studies. Blood samples are routinely used for Anaplasma spp. screening, but in persistently infected animals with intermittent or low-level bacteremia, other tissues might be useful. These guidelines have been developed as a direct outcome of the COST action TD1303 EURNEGVEC ("European Network of Neglected Vectors and Vector-Borne Diseases"). They review the direct laboratory tests (microscopy, nucleic acid-based detection and in vitro isolation) currently used for Anaplasma detection in ticks and vertebrates and their application.Mary Ann Liebert/Society for Zoonotic Ecology and Epidemiology (SocZEE)Repositório Científico do Instituto Nacional de SaúdeSilaghi, C.Santos, A.S.Gomes, J.Christova, I.Matei, I.A.Walder, G.Domingos, A.Bell-Sakyi, L.Sprong, H.von Loewenich, F.D.Oteo, J.A.de la Fuente, J.Dumler, J.S.2018-02-01T15:42:11Z2017-012017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/4902engVector Borne Zoonotic Dis. 2017 Jan;17(1):12-22.1530-366710.1089/vbz.2016.1960info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:40:22Zoai:repositorio.insa.pt:10400.18/4902Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:39:17.087409Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Guidelines for the Direct Detection of Anaplasma spp. in Diagnosis and Epidemiological Studies |
title |
Guidelines for the Direct Detection of Anaplasma spp. in Diagnosis and Epidemiological Studies |
spellingShingle |
Guidelines for the Direct Detection of Anaplasma spp. in Diagnosis and Epidemiological Studies Silaghi, C. Anaplasma spp. PCR Direct Diagnosis In vitro Isolation Microscopy Ticks Vertebrate Hosts Infecções Sistémicas e Zoonoses |
title_short |
Guidelines for the Direct Detection of Anaplasma spp. in Diagnosis and Epidemiological Studies |
title_full |
Guidelines for the Direct Detection of Anaplasma spp. in Diagnosis and Epidemiological Studies |
title_fullStr |
Guidelines for the Direct Detection of Anaplasma spp. in Diagnosis and Epidemiological Studies |
title_full_unstemmed |
Guidelines for the Direct Detection of Anaplasma spp. in Diagnosis and Epidemiological Studies |
title_sort |
Guidelines for the Direct Detection of Anaplasma spp. in Diagnosis and Epidemiological Studies |
author |
Silaghi, C. |
author_facet |
Silaghi, C. Santos, A.S. Gomes, J. Christova, I. Matei, I.A. Walder, G. Domingos, A. Bell-Sakyi, L. Sprong, H. von Loewenich, F.D. Oteo, J.A. de la Fuente, J. Dumler, J.S. |
author_role |
author |
author2 |
Santos, A.S. Gomes, J. Christova, I. Matei, I.A. Walder, G. Domingos, A. Bell-Sakyi, L. Sprong, H. von Loewenich, F.D. Oteo, J.A. de la Fuente, J. Dumler, J.S. |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
dc.contributor.author.fl_str_mv |
Silaghi, C. Santos, A.S. Gomes, J. Christova, I. Matei, I.A. Walder, G. Domingos, A. Bell-Sakyi, L. Sprong, H. von Loewenich, F.D. Oteo, J.A. de la Fuente, J. Dumler, J.S. |
dc.subject.por.fl_str_mv |
Anaplasma spp. PCR Direct Diagnosis In vitro Isolation Microscopy Ticks Vertebrate Hosts Infecções Sistémicas e Zoonoses |
topic |
Anaplasma spp. PCR Direct Diagnosis In vitro Isolation Microscopy Ticks Vertebrate Hosts Infecções Sistémicas e Zoonoses |
description |
The genus Anaplasma (Rickettsiales: Anaplasmataceae) comprises obligate intracellular Gram-negative bacteria that are mainly transmitted by ticks, and currently includes six species: Anaplasma bovis, Anaplasma centrale, Anaplasma marginale, Anaplasma phagocytophilum, Anaplasma platys, and Anaplasma ovis. These have long been known as etiological agents of veterinary diseases that affect domestic and wild animals worldwide. A zoonotic role has been recognized for A. phagocytophilum, but other species can also be pathogenic for humans. Anaplasma infections are usually challenging to diagnose, clinically presenting with nonspecific symptoms that vary greatly depending on the agent involved, the affected host, and other factors such as immune status and coinfections. The substantial economic impact associated with livestock infection and the growing number of human cases along with the risk of transfusion-transmitted infections, determines the need for accurate laboratory tests. Because hosts are usually seronegative in the initial phase of infection and serological cross-reactions with several Anaplasma species are observed after seroconversion, direct tests are the best approach for both case definition and epidemiological studies. Blood samples are routinely used for Anaplasma spp. screening, but in persistently infected animals with intermittent or low-level bacteremia, other tissues might be useful. These guidelines have been developed as a direct outcome of the COST action TD1303 EURNEGVEC ("European Network of Neglected Vectors and Vector-Borne Diseases"). They review the direct laboratory tests (microscopy, nucleic acid-based detection and in vitro isolation) currently used for Anaplasma detection in ticks and vertebrates and their application. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01 2017-01-01T00:00:00Z 2018-02-01T15:42:11Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.18/4902 |
url |
http://hdl.handle.net/10400.18/4902 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Vector Borne Zoonotic Dis. 2017 Jan;17(1):12-22. 1530-3667 10.1089/vbz.2016.1960 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/embargoedAccess |
eu_rights_str_mv |
embargoedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Mary Ann Liebert/Society for Zoonotic Ecology and Epidemiology (SocZEE) |
publisher.none.fl_str_mv |
Mary Ann Liebert/Society for Zoonotic Ecology and Epidemiology (SocZEE) |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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