Root-knot nematode feeding site development is impaired by cyclin-dependent kinase inhibitors

Detalhes bibliográficos
Autor(a) principal: Vieira, Paulo
Data de Publicação: 2012
Outros Autores: Engler, Gilbert, Veylder, Lieven, Mota, Manuel, Abad, Pierre, Engler, Janice
Tipo de documento: Artigo de conferência
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10174/7816
Resumo: Plant-parasitic nematodes of the genera Meloidogyne trigger the formation of giant cells that undergo recurring acytokinetic mitosis and endocycles. Expression analyses of key cell cycle genes showed their early induction in the nematode feeding site (NFS). Additionally, disturbance in NFS development and juvenile maturation were observed during treatment of infected roots with cell cycle inhibitors. Intense DNA synthesis and enlarged nuclei demonstrated that giant cells undergo additional endocycles. How precisely nematodes manipulate the cell cycle in their favour remains to be understood. A systematic comparison of the temporal and spatial expression pattern of core cell cycle genes between uninfected roots and in galls of Arabidopsis thaliana resulted in the identification of a collection of genes up- or downregulated in NFC. Among them, negative regulators are candidates to control the cell cycle in NFC. Previous work has shown that KRP2, a member of the cyclin-dependent kinase/kip-related proteins (ICK/KRP), regulate mitosis-to-endocycle transition in plant cells, and is expressed in endoreduplicating cells. The KRP2 gene showed to be expressed during gall development. Therefore to study the relevance of the KRP cell cycle inhibitor genes (7 in Arabidopis) for NFS ontogeny, mutant lines over-expressing and knocked-out are being tested to determine their effect on feeding site development. In vivo subcellular localization studies have been carried out to better understand the dynamics of these proteins during giant cell development. Based on these data, three KRP genes are perceived to control giant cell size and consequently nematode reproduction.
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spelling Root-knot nematode feeding site development is impaired by cyclin-dependent kinase inhibitorsMeloidogyne incognitaArabidopiscell-cyclePlant-parasitic nematodes of the genera Meloidogyne trigger the formation of giant cells that undergo recurring acytokinetic mitosis and endocycles. Expression analyses of key cell cycle genes showed their early induction in the nematode feeding site (NFS). Additionally, disturbance in NFS development and juvenile maturation were observed during treatment of infected roots with cell cycle inhibitors. Intense DNA synthesis and enlarged nuclei demonstrated that giant cells undergo additional endocycles. How precisely nematodes manipulate the cell cycle in their favour remains to be understood. A systematic comparison of the temporal and spatial expression pattern of core cell cycle genes between uninfected roots and in galls of Arabidopsis thaliana resulted in the identification of a collection of genes up- or downregulated in NFC. Among them, negative regulators are candidates to control the cell cycle in NFC. Previous work has shown that KRP2, a member of the cyclin-dependent kinase/kip-related proteins (ICK/KRP), regulate mitosis-to-endocycle transition in plant cells, and is expressed in endoreduplicating cells. The KRP2 gene showed to be expressed during gall development. Therefore to study the relevance of the KRP cell cycle inhibitor genes (7 in Arabidopis) for NFS ontogeny, mutant lines over-expressing and knocked-out are being tested to determine their effect on feeding site development. In vivo subcellular localization studies have been carried out to better understand the dynamics of these proteins during giant cell development. Based on these data, three KRP genes are perceived to control giant cell size and consequently nematode reproduction.2013-01-28T14:56:53Z2013-01-282012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjecthttp://hdl.handle.net/10174/7816http://hdl.handle.net/10174/7816engnaonaosimICAAMpvieira@uevora.ptndndmmota@uevora.ptndnd218Vieira, PauloEngler, GilbertVeylder, LievenMota, ManuelAbad, PierreEngler, Janiceinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T18:48:28Zoai:dspace.uevora.pt:10174/7816Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:02:19.347034Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Root-knot nematode feeding site development is impaired by cyclin-dependent kinase inhibitors
title Root-knot nematode feeding site development is impaired by cyclin-dependent kinase inhibitors
spellingShingle Root-knot nematode feeding site development is impaired by cyclin-dependent kinase inhibitors
Vieira, Paulo
Meloidogyne incognita
Arabidopis
cell-cycle
title_short Root-knot nematode feeding site development is impaired by cyclin-dependent kinase inhibitors
title_full Root-knot nematode feeding site development is impaired by cyclin-dependent kinase inhibitors
title_fullStr Root-knot nematode feeding site development is impaired by cyclin-dependent kinase inhibitors
title_full_unstemmed Root-knot nematode feeding site development is impaired by cyclin-dependent kinase inhibitors
title_sort Root-knot nematode feeding site development is impaired by cyclin-dependent kinase inhibitors
author Vieira, Paulo
author_facet Vieira, Paulo
Engler, Gilbert
Veylder, Lieven
Mota, Manuel
Abad, Pierre
Engler, Janice
author_role author
author2 Engler, Gilbert
Veylder, Lieven
Mota, Manuel
Abad, Pierre
Engler, Janice
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Vieira, Paulo
Engler, Gilbert
Veylder, Lieven
Mota, Manuel
Abad, Pierre
Engler, Janice
dc.subject.por.fl_str_mv Meloidogyne incognita
Arabidopis
cell-cycle
topic Meloidogyne incognita
Arabidopis
cell-cycle
description Plant-parasitic nematodes of the genera Meloidogyne trigger the formation of giant cells that undergo recurring acytokinetic mitosis and endocycles. Expression analyses of key cell cycle genes showed their early induction in the nematode feeding site (NFS). Additionally, disturbance in NFS development and juvenile maturation were observed during treatment of infected roots with cell cycle inhibitors. Intense DNA synthesis and enlarged nuclei demonstrated that giant cells undergo additional endocycles. How precisely nematodes manipulate the cell cycle in their favour remains to be understood. A systematic comparison of the temporal and spatial expression pattern of core cell cycle genes between uninfected roots and in galls of Arabidopsis thaliana resulted in the identification of a collection of genes up- or downregulated in NFC. Among them, negative regulators are candidates to control the cell cycle in NFC. Previous work has shown that KRP2, a member of the cyclin-dependent kinase/kip-related proteins (ICK/KRP), regulate mitosis-to-endocycle transition in plant cells, and is expressed in endoreduplicating cells. The KRP2 gene showed to be expressed during gall development. Therefore to study the relevance of the KRP cell cycle inhibitor genes (7 in Arabidopis) for NFS ontogeny, mutant lines over-expressing and knocked-out are being tested to determine their effect on feeding site development. In vivo subcellular localization studies have been carried out to better understand the dynamics of these proteins during giant cell development. Based on these data, three KRP genes are perceived to control giant cell size and consequently nematode reproduction.
publishDate 2012
dc.date.none.fl_str_mv 2012-01-01T00:00:00Z
2013-01-28T14:56:53Z
2013-01-28
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