Protein extraction and comparison of stain protocols for analysis of two-dimensional electrophoresis gels

Detalhes bibliográficos
Autor(a) principal: Silva, Joana
Data de Publicação: 2004
Outros Autores: Carvalho, Ana Sofia, Votorino, Rui, Domingues, Pedro, Teixeira, Paula, Gibbs, paul
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.14/6688
Resumo: Protein extraction and the proteome of Lactobacillus delbrueckii subsp. bulgaricus were studied using different stains. The reversible silver staining technique was shown to be more sensitive than the irreversible silver stain. Coomassie colloidal was demonstrated to be as sensitive as reversible silver stain; however, the Coomassie colloidal blue solution developed a higher background and for sample preparation was more time-consuming.
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spelling Protein extraction and comparison of stain protocols for analysis of two-dimensional electrophoresis gelsLactobacillus bulgaricusProtein extraction2-DE electrophoresisStainingSensitivityProtein extraction and the proteome of Lactobacillus delbrueckii subsp. bulgaricus were studied using different stains. The reversible silver staining technique was shown to be more sensitive than the irreversible silver stain. Coomassie colloidal was demonstrated to be as sensitive as reversible silver stain; however, the Coomassie colloidal blue solution developed a higher background and for sample preparation was more time-consuming.Bentham Science Publishers Ltd.Veritati - Repositório Institucional da Universidade Católica PortuguesaSilva, JoanaCarvalho, Ana SofiaVotorino, RuiDomingues, PedroTeixeira, PaulaGibbs, paul2011-10-21T14:08:22Z20042004-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/6688eng"Protein and peptide letters" . ISSN 0929-866511:2 (2004) 189-194info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-09-06T11:59:13Zoai:repositorio.ucp.pt:10400.14/6688Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-09-06T11:59:13Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Protein extraction and comparison of stain protocols for analysis of two-dimensional electrophoresis gels
title Protein extraction and comparison of stain protocols for analysis of two-dimensional electrophoresis gels
spellingShingle Protein extraction and comparison of stain protocols for analysis of two-dimensional electrophoresis gels
Silva, Joana
Lactobacillus bulgaricus
Protein extraction
2-DE electrophoresis
Staining
Sensitivity
title_short Protein extraction and comparison of stain protocols for analysis of two-dimensional electrophoresis gels
title_full Protein extraction and comparison of stain protocols for analysis of two-dimensional electrophoresis gels
title_fullStr Protein extraction and comparison of stain protocols for analysis of two-dimensional electrophoresis gels
title_full_unstemmed Protein extraction and comparison of stain protocols for analysis of two-dimensional electrophoresis gels
title_sort Protein extraction and comparison of stain protocols for analysis of two-dimensional electrophoresis gels
author Silva, Joana
author_facet Silva, Joana
Carvalho, Ana Sofia
Votorino, Rui
Domingues, Pedro
Teixeira, Paula
Gibbs, paul
author_role author
author2 Carvalho, Ana Sofia
Votorino, Rui
Domingues, Pedro
Teixeira, Paula
Gibbs, paul
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Veritati - Repositório Institucional da Universidade Católica Portuguesa
dc.contributor.author.fl_str_mv Silva, Joana
Carvalho, Ana Sofia
Votorino, Rui
Domingues, Pedro
Teixeira, Paula
Gibbs, paul
dc.subject.por.fl_str_mv Lactobacillus bulgaricus
Protein extraction
2-DE electrophoresis
Staining
Sensitivity
topic Lactobacillus bulgaricus
Protein extraction
2-DE electrophoresis
Staining
Sensitivity
description Protein extraction and the proteome of Lactobacillus delbrueckii subsp. bulgaricus were studied using different stains. The reversible silver staining technique was shown to be more sensitive than the irreversible silver stain. Coomassie colloidal was demonstrated to be as sensitive as reversible silver stain; however, the Coomassie colloidal blue solution developed a higher background and for sample preparation was more time-consuming.
publishDate 2004
dc.date.none.fl_str_mv 2004
2004-01-01T00:00:00Z
2011-10-21T14:08:22Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.14/6688
url http://hdl.handle.net/10400.14/6688
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Protein and peptide letters" . ISSN 0929-866511:2 (2004) 189-194
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Bentham Science Publishers Ltd.
publisher.none.fl_str_mv Bentham Science Publishers Ltd.
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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