Enzymatic identification of Glyptapanteles sp. (Insecta: Hymenoptera) from Madeira Island

Detalhes bibliográficos
Autor(a) principal: Oliveira, Luísa
Data de Publicação: 2001
Outros Autores: Vieira, Virgílio, Tavares, João, Garcia, Patrícia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.3/830
Resumo: During a scientific expedition carried out in Madeira Island in September 1997, Pseudaletia (=Mythimna) unipuncta (Lepidoptera: Noctuidae) larvae were collected in maize fields and pastures. These larvae were parasitized by a braconid belonging to Glyptapanteles genus. This population was characterised biochemically to identify the species. Seven enzyme systems studied by electrophoresis were analysed: aldehyde oxidase (AO), aglycerophosphate dehydrogenase (a-GPD), tetrazolium oxidase (TO), malate dehydrogenase (MDH), glucose-6-dehydrogenase (G6PD), malic enzyme (ME), isocitrate dehydrogenase (IDH). All systems showed only one band, with two exceptions: a-GPD and ME which had two bands which corresponded to different loci. No polymorphic enzymes were detected. Comparing this results with those obtained from G. militaris collected in Azores Islands and in Quebec-Canada we can suggest that the population of Glyptapanteles collected in Madeira Island belongs to G. militaris species.
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spelling Enzymatic identification of Glyptapanteles sp. (Insecta: Hymenoptera) from Madeira IslandEnzymatic IdentificationGlyptapanteles militarisMadeira IslandDuring a scientific expedition carried out in Madeira Island in September 1997, Pseudaletia (=Mythimna) unipuncta (Lepidoptera: Noctuidae) larvae were collected in maize fields and pastures. These larvae were parasitized by a braconid belonging to Glyptapanteles genus. This population was characterised biochemically to identify the species. Seven enzyme systems studied by electrophoresis were analysed: aldehyde oxidase (AO), aglycerophosphate dehydrogenase (a-GPD), tetrazolium oxidase (TO), malate dehydrogenase (MDH), glucose-6-dehydrogenase (G6PD), malic enzyme (ME), isocitrate dehydrogenase (IDH). All systems showed only one band, with two exceptions: a-GPD and ME which had two bands which corresponded to different loci. No polymorphic enzymes were detected. Comparing this results with those obtained from G. militaris collected in Azores Islands and in Quebec-Canada we can suggest that the population of Glyptapanteles collected in Madeira Island belongs to G. militaris species.Universidade dos AçoresRepositório da Universidade dos AçoresOliveira, LuísaVieira, VirgílioTavares, JoãoGarcia, Patrícia2011-01-18T15:19:45Z20012001-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.3/830engOLIVEIRA, L., V. VIEIRA, J. TAVARES & P. GARCIA, (2001). Enzymatic identification of Glyptapanteles militaris (Hymenoptera: Braconidae) from Madeira Island. “Arquipélago, Life and Marine Sciences”, Supl. 2(B): 39-42.0873-4704info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-12-20T14:28:21Zoai:repositorio.uac.pt:10400.3/830Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:23:49.023549Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Enzymatic identification of Glyptapanteles sp. (Insecta: Hymenoptera) from Madeira Island
title Enzymatic identification of Glyptapanteles sp. (Insecta: Hymenoptera) from Madeira Island
spellingShingle Enzymatic identification of Glyptapanteles sp. (Insecta: Hymenoptera) from Madeira Island
Oliveira, Luísa
Enzymatic Identification
Glyptapanteles militaris
Madeira Island
title_short Enzymatic identification of Glyptapanteles sp. (Insecta: Hymenoptera) from Madeira Island
title_full Enzymatic identification of Glyptapanteles sp. (Insecta: Hymenoptera) from Madeira Island
title_fullStr Enzymatic identification of Glyptapanteles sp. (Insecta: Hymenoptera) from Madeira Island
title_full_unstemmed Enzymatic identification of Glyptapanteles sp. (Insecta: Hymenoptera) from Madeira Island
title_sort Enzymatic identification of Glyptapanteles sp. (Insecta: Hymenoptera) from Madeira Island
author Oliveira, Luísa
author_facet Oliveira, Luísa
Vieira, Virgílio
Tavares, João
Garcia, Patrícia
author_role author
author2 Vieira, Virgílio
Tavares, João
Garcia, Patrícia
author2_role author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade dos Açores
dc.contributor.author.fl_str_mv Oliveira, Luísa
Vieira, Virgílio
Tavares, João
Garcia, Patrícia
dc.subject.por.fl_str_mv Enzymatic Identification
Glyptapanteles militaris
Madeira Island
topic Enzymatic Identification
Glyptapanteles militaris
Madeira Island
description During a scientific expedition carried out in Madeira Island in September 1997, Pseudaletia (=Mythimna) unipuncta (Lepidoptera: Noctuidae) larvae were collected in maize fields and pastures. These larvae were parasitized by a braconid belonging to Glyptapanteles genus. This population was characterised biochemically to identify the species. Seven enzyme systems studied by electrophoresis were analysed: aldehyde oxidase (AO), aglycerophosphate dehydrogenase (a-GPD), tetrazolium oxidase (TO), malate dehydrogenase (MDH), glucose-6-dehydrogenase (G6PD), malic enzyme (ME), isocitrate dehydrogenase (IDH). All systems showed only one band, with two exceptions: a-GPD and ME which had two bands which corresponded to different loci. No polymorphic enzymes were detected. Comparing this results with those obtained from G. militaris collected in Azores Islands and in Quebec-Canada we can suggest that the population of Glyptapanteles collected in Madeira Island belongs to G. militaris species.
publishDate 2001
dc.date.none.fl_str_mv 2001
2001-01-01T00:00:00Z
2011-01-18T15:19:45Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.3/830
url http://hdl.handle.net/10400.3/830
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv OLIVEIRA, L., V. VIEIRA, J. TAVARES & P. GARCIA, (2001). Enzymatic identification of Glyptapanteles militaris (Hymenoptera: Braconidae) from Madeira Island. “Arquipélago, Life and Marine Sciences”, Supl. 2(B): 39-42.
0873-4704
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Universidade dos Açores
publisher.none.fl_str_mv Universidade dos Açores
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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