Saccharomyces cerevisiae cells lacking the zinc vacuolar transporter Zrt3 display improved ethanol productivity in lignocellulosic hydrolysates
Autor(a) principal: | |
---|---|
Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/75597 |
Resumo: | Yeast-based bioethanol production from lignocellulosic hydrolysates (LH) is an attractive and sustainable alternative for biofuel production. However, the presence of acetic acid (AA) in LH is still a major problem. Indeed, above certain concentrations, AA inhibits yeast fermentation and triggers a regulated cell death (RCD) process mediated by the mitochondria and vacuole. Understanding the mechanisms involved in AA-induced RCD (AA-RCD) may thus help select robust fermentative yeast strains, providing novel insights to improve lignocellulosic ethanol (LE) production. Herein, we hypothesized that zinc vacuolar transporters are involved in vacuole-mediated AA-RCD, since zinc enhances ethanol production and zinc-dependent catalase and superoxide dismutase protect from AA-RCD. In this work, zinc limitation sensitized wild-type cells to AA-RCD, while zinc supplementation resulted in a small protective effect. Cells lacking the vacuolar zinc transporter Zrt3 were highly resistant to AA-RCD, exhibiting reduced vacuolar dysfunction. Moreover, zrt3Δ cells displayed higher ethanol productivity than their wild-type counterparts, both when cultivated in rich medium with AA (0.29 g L−1 h−1 versus 0.11 g L−1 h−1) and in an LH (0.73 g L−1 h−1 versus 0.55 g L−1 h−1). Overall, the deletion of ZRT3 emerges as a promising strategy to increase strain robustness in LE industrial production. |
id |
RCAP_d4102a914a8024f4a5d0ed7ec25b0709 |
---|---|
oai_identifier_str |
oai:repositorium.sdum.uminho.pt:1822/75597 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Saccharomyces cerevisiae cells lacking the zinc vacuolar transporter Zrt3 display improved ethanol productivity in lignocellulosic hydrolysatesSaccharomyces cerevisiaeAcetic acidLignocellulosic hydrolysatesVacuolar zinc transporterLignocellulosic ethanolScience & TechnologyYeast-based bioethanol production from lignocellulosic hydrolysates (LH) is an attractive and sustainable alternative for biofuel production. However, the presence of acetic acid (AA) in LH is still a major problem. Indeed, above certain concentrations, AA inhibits yeast fermentation and triggers a regulated cell death (RCD) process mediated by the mitochondria and vacuole. Understanding the mechanisms involved in AA-induced RCD (AA-RCD) may thus help select robust fermentative yeast strains, providing novel insights to improve lignocellulosic ethanol (LE) production. Herein, we hypothesized that zinc vacuolar transporters are involved in vacuole-mediated AA-RCD, since zinc enhances ethanol production and zinc-dependent catalase and superoxide dismutase protect from AA-RCD. In this work, zinc limitation sensitized wild-type cells to AA-RCD, while zinc supplementation resulted in a small protective effect. Cells lacking the vacuolar zinc transporter Zrt3 were highly resistant to AA-RCD, exhibiting reduced vacuolar dysfunction. Moreover, zrt3Δ cells displayed higher ethanol productivity than their wild-type counterparts, both when cultivated in rich medium with AA (0.29 g L−1 h−1 versus 0.11 g L−1 h−1) and in an LH (0.73 g L−1 h−1 versus 0.55 g L−1 h−1). Overall, the deletion of ZRT3 emerges as a promising strategy to increase strain robustness in LE industrial production.This work was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of “Contrato-Programa” UIDB/04050/2020 - PE 20-23 and UIDB/04469/2020 unit. Cátia Santos-Pereira acknowledges the Ph.D. fellowship PD/BD/128032/2016 funded by FCT under the scope of the doctoral program in Applied and Environmental Microbiology (DP_AEM).The authors acknowledge Helena Pereira for constructing the pRS413-Pep4- mCherry plasmid and Sara L. Baptista for the preparation of vine prune residue autohydrolysis liquors. BIOVINO project (0688_BIOVINO_6_E) funded by INTERREG España—Portugal and European Regional Development Fund (ERDF) is acknowledged for the supply of vine prune residuesinfo:eu-repo/semantics/publishedVersionMultidisciplinary Digital Publishing Institute (MDPI)Universidade do MinhoTerra-Matos, JoanaTeixeira, Marta OliveiraPereira, Cátia Sofia SantosNoronha, HenriqueDomingues, LucíliaSieiro, CarmenGerós, H.Chaves, Susana Alexandra RodriguesSousa, Maria JoãoCôrte-Real, Manuela2022-01-142022-01-14T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/75597engTerra-Matos, Joana; Teixeira, Marta Oliveira; Cátia S. Pereira; Noronha, Henrique; Domingues, Lucília; Sieiro, Carmen; Gerós, Hernâni; Chaves, Susana Rodrigues; Sousa, Maria João; Côrte-Real, Manuela, Saccharomyces cerevisiae cells lacking the zinc vacuolar transporter Zrt3 display improved ethanol productivity in lignocellulosic hydrolysates. Journal of Fungi, 8(1), 78, 20222309-608X10.3390/jof8010078http://www.mdpi.com/journal/jofinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T05:55:18Zoai:repositorium.sdum.uminho.pt:1822/75597Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T05:55:18Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Saccharomyces cerevisiae cells lacking the zinc vacuolar transporter Zrt3 display improved ethanol productivity in lignocellulosic hydrolysates |
title |
Saccharomyces cerevisiae cells lacking the zinc vacuolar transporter Zrt3 display improved ethanol productivity in lignocellulosic hydrolysates |
spellingShingle |
Saccharomyces cerevisiae cells lacking the zinc vacuolar transporter Zrt3 display improved ethanol productivity in lignocellulosic hydrolysates Terra-Matos, Joana Saccharomyces cerevisiae Acetic acid Lignocellulosic hydrolysates Vacuolar zinc transporter Lignocellulosic ethanol Science & Technology |
title_short |
Saccharomyces cerevisiae cells lacking the zinc vacuolar transporter Zrt3 display improved ethanol productivity in lignocellulosic hydrolysates |
title_full |
Saccharomyces cerevisiae cells lacking the zinc vacuolar transporter Zrt3 display improved ethanol productivity in lignocellulosic hydrolysates |
title_fullStr |
Saccharomyces cerevisiae cells lacking the zinc vacuolar transporter Zrt3 display improved ethanol productivity in lignocellulosic hydrolysates |
title_full_unstemmed |
Saccharomyces cerevisiae cells lacking the zinc vacuolar transporter Zrt3 display improved ethanol productivity in lignocellulosic hydrolysates |
title_sort |
Saccharomyces cerevisiae cells lacking the zinc vacuolar transporter Zrt3 display improved ethanol productivity in lignocellulosic hydrolysates |
author |
Terra-Matos, Joana |
author_facet |
Terra-Matos, Joana Teixeira, Marta Oliveira Pereira, Cátia Sofia Santos Noronha, Henrique Domingues, Lucília Sieiro, Carmen Gerós, H. Chaves, Susana Alexandra Rodrigues Sousa, Maria João Côrte-Real, Manuela |
author_role |
author |
author2 |
Teixeira, Marta Oliveira Pereira, Cátia Sofia Santos Noronha, Henrique Domingues, Lucília Sieiro, Carmen Gerós, H. Chaves, Susana Alexandra Rodrigues Sousa, Maria João Côrte-Real, Manuela |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Terra-Matos, Joana Teixeira, Marta Oliveira Pereira, Cátia Sofia Santos Noronha, Henrique Domingues, Lucília Sieiro, Carmen Gerós, H. Chaves, Susana Alexandra Rodrigues Sousa, Maria João Côrte-Real, Manuela |
dc.subject.por.fl_str_mv |
Saccharomyces cerevisiae Acetic acid Lignocellulosic hydrolysates Vacuolar zinc transporter Lignocellulosic ethanol Science & Technology |
topic |
Saccharomyces cerevisiae Acetic acid Lignocellulosic hydrolysates Vacuolar zinc transporter Lignocellulosic ethanol Science & Technology |
description |
Yeast-based bioethanol production from lignocellulosic hydrolysates (LH) is an attractive and sustainable alternative for biofuel production. However, the presence of acetic acid (AA) in LH is still a major problem. Indeed, above certain concentrations, AA inhibits yeast fermentation and triggers a regulated cell death (RCD) process mediated by the mitochondria and vacuole. Understanding the mechanisms involved in AA-induced RCD (AA-RCD) may thus help select robust fermentative yeast strains, providing novel insights to improve lignocellulosic ethanol (LE) production. Herein, we hypothesized that zinc vacuolar transporters are involved in vacuole-mediated AA-RCD, since zinc enhances ethanol production and zinc-dependent catalase and superoxide dismutase protect from AA-RCD. In this work, zinc limitation sensitized wild-type cells to AA-RCD, while zinc supplementation resulted in a small protective effect. Cells lacking the vacuolar zinc transporter Zrt3 were highly resistant to AA-RCD, exhibiting reduced vacuolar dysfunction. Moreover, zrt3Δ cells displayed higher ethanol productivity than their wild-type counterparts, both when cultivated in rich medium with AA (0.29 g L−1 h−1 versus 0.11 g L−1 h−1) and in an LH (0.73 g L−1 h−1 versus 0.55 g L−1 h−1). Overall, the deletion of ZRT3 emerges as a promising strategy to increase strain robustness in LE industrial production. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-14 2022-01-14T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/75597 |
url |
https://hdl.handle.net/1822/75597 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Terra-Matos, Joana; Teixeira, Marta Oliveira; Cátia S. Pereira; Noronha, Henrique; Domingues, Lucília; Sieiro, Carmen; Gerós, Hernâni; Chaves, Susana Rodrigues; Sousa, Maria João; Côrte-Real, Manuela, Saccharomyces cerevisiae cells lacking the zinc vacuolar transporter Zrt3 display improved ethanol productivity in lignocellulosic hydrolysates. Journal of Fungi, 8(1), 78, 2022 2309-608X 10.3390/jof8010078 http://www.mdpi.com/journal/jof |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute (MDPI) |
publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute (MDPI) |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
_version_ |
1817544787242254336 |