Second-generation environmental sequencing unmasks marine metazoan biodiversity
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.1/5488 |
Resumo: | Biodiversity is of crucial importance for ecosystem functioning, sustainability and resilience, but the magnitude and organization of marine diversity at a range of spatial and taxonomic scales are undefined. In this paper, we use second-generation sequencing to unmask putatively diverse marine metazoan biodiversity in a Scottish temperate benthic ecosystem. We show that remarkable differences in diversity occurred at microgeographical scales and refute currently accepted ecological and taxonomic paradigms of meiofaunal identity, rank abundance and concomitant understanding of trophic dynamics. Richness estimates from the current benchmarked Operational Clustering of Taxonomic Units from Parallel UltraSequencing analyses are broadly aligned with those derived from morphological assessments. However, the slope of taxon rarefaction curves for many phyla remains incomplete, suggesting that the true alpha diversity is likely to exceed current perceptions. The approaches provide a rapid, objective and cost-effective taxonomic framework for exploring links between ecosystem structure and function of all hitherto intractable, but ecologically important, communities. |
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Second-generation environmental sequencing unmasks marine metazoan biodiversityBiodiversity is of crucial importance for ecosystem functioning, sustainability and resilience, but the magnitude and organization of marine diversity at a range of spatial and taxonomic scales are undefined. In this paper, we use second-generation sequencing to unmask putatively diverse marine metazoan biodiversity in a Scottish temperate benthic ecosystem. We show that remarkable differences in diversity occurred at microgeographical scales and refute currently accepted ecological and taxonomic paradigms of meiofaunal identity, rank abundance and concomitant understanding of trophic dynamics. Richness estimates from the current benchmarked Operational Clustering of Taxonomic Units from Parallel UltraSequencing analyses are broadly aligned with those derived from morphological assessments. However, the slope of taxon rarefaction curves for many phyla remains incomplete, suggesting that the true alpha diversity is likely to exceed current perceptions. The approaches provide a rapid, objective and cost-effective taxonomic framework for exploring links between ecosystem structure and function of all hitherto intractable, but ecologically important, communities.Nature Publishing GroupSapientiaFonseca, V. G.Carvalho, GarySung, W.Johnson, Harriet F.Power, DeborahNeill, Simon P.Packer, MargaretBlaxter, M. L.Lambshead, P. J. D.Thomas, W. K.Creer, S.2014-10-24T13:15:35Z20102010-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/5488engVera G. Fonse, Gary R. Carvalho, Way Sung, Harriet F. Johnson, Deborah M. Power, Simon P. Neill, Margaret Packer, Mark L. Blaxter, P. John D. Lambshead, W. Kelley Thomas & Simon Creer, "Second-generation environmental sequencing unmasks marine metazoan biodiversity" in Nature Communications 1, Article number: 982041-1723AUT: DPO00386http://dx.doi.org/10.1038/ncomms1095info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:16:42Zoai:sapientia.ualg.pt:10400.1/5488Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:58:30.927097Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Second-generation environmental sequencing unmasks marine metazoan biodiversity |
title |
Second-generation environmental sequencing unmasks marine metazoan biodiversity |
spellingShingle |
Second-generation environmental sequencing unmasks marine metazoan biodiversity Fonseca, V. G. |
title_short |
Second-generation environmental sequencing unmasks marine metazoan biodiversity |
title_full |
Second-generation environmental sequencing unmasks marine metazoan biodiversity |
title_fullStr |
Second-generation environmental sequencing unmasks marine metazoan biodiversity |
title_full_unstemmed |
Second-generation environmental sequencing unmasks marine metazoan biodiversity |
title_sort |
Second-generation environmental sequencing unmasks marine metazoan biodiversity |
author |
Fonseca, V. G. |
author_facet |
Fonseca, V. G. Carvalho, Gary Sung, W. Johnson, Harriet F. Power, Deborah Neill, Simon P. Packer, Margaret Blaxter, M. L. Lambshead, P. J. D. Thomas, W. K. Creer, S. |
author_role |
author |
author2 |
Carvalho, Gary Sung, W. Johnson, Harriet F. Power, Deborah Neill, Simon P. Packer, Margaret Blaxter, M. L. Lambshead, P. J. D. Thomas, W. K. Creer, S. |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Sapientia |
dc.contributor.author.fl_str_mv |
Fonseca, V. G. Carvalho, Gary Sung, W. Johnson, Harriet F. Power, Deborah Neill, Simon P. Packer, Margaret Blaxter, M. L. Lambshead, P. J. D. Thomas, W. K. Creer, S. |
description |
Biodiversity is of crucial importance for ecosystem functioning, sustainability and resilience, but the magnitude and organization of marine diversity at a range of spatial and taxonomic scales are undefined. In this paper, we use second-generation sequencing to unmask putatively diverse marine metazoan biodiversity in a Scottish temperate benthic ecosystem. We show that remarkable differences in diversity occurred at microgeographical scales and refute currently accepted ecological and taxonomic paradigms of meiofaunal identity, rank abundance and concomitant understanding of trophic dynamics. Richness estimates from the current benchmarked Operational Clustering of Taxonomic Units from Parallel UltraSequencing analyses are broadly aligned with those derived from morphological assessments. However, the slope of taxon rarefaction curves for many phyla remains incomplete, suggesting that the true alpha diversity is likely to exceed current perceptions. The approaches provide a rapid, objective and cost-effective taxonomic framework for exploring links between ecosystem structure and function of all hitherto intractable, but ecologically important, communities. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010 2010-01-01T00:00:00Z 2014-10-24T13:15:35Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.1/5488 |
url |
http://hdl.handle.net/10400.1/5488 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Vera G. Fonse, Gary R. Carvalho, Way Sung, Harriet F. Johnson, Deborah M. Power, Simon P. Neill, Margaret Packer, Mark L. Blaxter, P. John D. Lambshead, W. Kelley Thomas & Simon Creer, "Second-generation environmental sequencing unmasks marine metazoan biodiversity" in Nature Communications 1, Article number: 98 2041-1723 AUT: DPO00386 http://dx.doi.org/10.1038/ncomms1095 |
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info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Nature Publishing Group |
publisher.none.fl_str_mv |
Nature Publishing Group |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133202266193920 |