Development of a piezoelectric biosensor based on PVDF films

Detalhes bibliográficos
Autor(a) principal: Marques, Ana Raquel Correia Gonçalves
Data de Publicação: 2011
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/6575
Resumo: Dissertação para obtenção do Grau de Mestre em Engenharia Química e Bioquímica
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spelling Development of a piezoelectric biosensor based on PVDF filmsBiosensorPVDFPiezoelectricityPVDF membranesSurface modificationDissertação para obtenção do Grau de Mestre em Engenharia Química e BioquímicaThe core of this work is a piezoelectric biosensor in which acoustic waves are launched in very thin PVDF polymer films to produce an oscillatory resonant device. The essence of the device consists in a polymer film system made of a piezoelectric polymer, PVDF, responsible for the film oscillation and a porous membrane, Immobilon, a special type of porous PVDF with protein binding capacity which can act as a biosensitive area. The possibility of using a film system composed only by PVDF was studied. Due to its strong hydrophobic nature, surface modification was aimed to be performed by coating a functional layer on the membrane surface, in order to improve the hydrophilicity and biocompatibility of PVDF. An immersion method was preformed and applied to porous and non-porous PVDF membranes. Three distinct coating solutions were studied, namely, polyethylene glycol (PEG), 3,4-Dihydroxy-DL-phenylalanine (DL-DOPA) and Gum Arabic solutions. Hydrophilicity improvements of the membranes were characterized by water contact angle measurements and its elemental composition was studied by elemental microanalysis. BSA protein marked with FITC fluorescein was used to perform quantitative and qualitative assays in order to study its adsorption to coated and uncoated PVDF membranes. Globally, the best results were obtained when a solution of PEG was utilized with 0.2 μm microporous membrane. The sensor response was tested with polyethylene glycol (PEG) and bovine serum albumin (BSA), using a film system composed by PVDF/0.2 μm Immobilon/PVDF, which was able to respond to the presence of both compounds in liquid medium.Faculdade de Ciências e TecnologiaRoque, AnaGomes, PedroRUNMarques, Ana Raquel Correia Gonçalves2011-12-28T15:20:35Z20112011-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/6575enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:37:40Zoai:run.unl.pt:10362/6575Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:16:52.792259Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Development of a piezoelectric biosensor based on PVDF films
title Development of a piezoelectric biosensor based on PVDF films
spellingShingle Development of a piezoelectric biosensor based on PVDF films
Marques, Ana Raquel Correia Gonçalves
Biosensor
PVDF
Piezoelectricity
PVDF membranes
Surface modification
title_short Development of a piezoelectric biosensor based on PVDF films
title_full Development of a piezoelectric biosensor based on PVDF films
title_fullStr Development of a piezoelectric biosensor based on PVDF films
title_full_unstemmed Development of a piezoelectric biosensor based on PVDF films
title_sort Development of a piezoelectric biosensor based on PVDF films
author Marques, Ana Raquel Correia Gonçalves
author_facet Marques, Ana Raquel Correia Gonçalves
author_role author
dc.contributor.none.fl_str_mv Roque, Ana
Gomes, Pedro
RUN
dc.contributor.author.fl_str_mv Marques, Ana Raquel Correia Gonçalves
dc.subject.por.fl_str_mv Biosensor
PVDF
Piezoelectricity
PVDF membranes
Surface modification
topic Biosensor
PVDF
Piezoelectricity
PVDF membranes
Surface modification
description Dissertação para obtenção do Grau de Mestre em Engenharia Química e Bioquímica
publishDate 2011
dc.date.none.fl_str_mv 2011-12-28T15:20:35Z
2011
2011-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/6575
url http://hdl.handle.net/10362/6575
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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