Phenolic profile and bioactive properties of carissa macrocarpa (Eckl.) A.DC.: An in vitro comparative study between leaves, stems, and flowers
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/20086 |
Resumo: | The present work aimed to characterize leaves, stems, and flowers of Carissa macrocarpa (Eckl.) A.DC., by performing an analysis of the phenolic compounds by HPLC-DAD/ESI-MS, correlating them with bioactive properties, such as antioxidant, anti-inflammatory, cytotoxic, and antimicrobial activities. Thirty polyphenols were identified in the hydroethanolic extract, including phenolic acids, flavan-3-ols, and flavonol glycosides derivatives (which presented the highest number of identified compounds). However, flavan-3-ols showed the highest concentration in stems (mainly owing to the presence of dimers, trimmers, and tetramers of type B (epi)catechin). Leaves were distinguished by their high antioxidant and anti-inflammatory activities, as well as their bactericidal e ect against E. coli, while stems presented a higher cytotoxic activity and bactericidal e ect against Gram-positive bacteria. Moreover, a high correlation between the studied bioactivities and the presence of phenolic compounds was also verified. The obtained results bring added value to the studied plant species. |
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Phenolic profile and bioactive properties of carissa macrocarpa (Eckl.) A.DC.: An in vitro comparative study between leaves, stems, and flowersBioactivitiesCarissa macrocarpaPearson's correlationPhenolic profilePlant aerial partsThe present work aimed to characterize leaves, stems, and flowers of Carissa macrocarpa (Eckl.) A.DC., by performing an analysis of the phenolic compounds by HPLC-DAD/ESI-MS, correlating them with bioactive properties, such as antioxidant, anti-inflammatory, cytotoxic, and antimicrobial activities. Thirty polyphenols were identified in the hydroethanolic extract, including phenolic acids, flavan-3-ols, and flavonol glycosides derivatives (which presented the highest number of identified compounds). However, flavan-3-ols showed the highest concentration in stems (mainly owing to the presence of dimers, trimmers, and tetramers of type B (epi)catechin). Leaves were distinguished by their high antioxidant and anti-inflammatory activities, as well as their bactericidal e ect against E. coli, while stems presented a higher cytotoxic activity and bactericidal e ect against Gram-positive bacteria. Moreover, a high correlation between the studied bioactivities and the presence of phenolic compounds was also verified. The obtained results bring added value to the studied plant species.The authors are grateful to the Foundation for Science and Technology (FCT, Portugal) and FEDER under Programmer PT2020 for financial support to CIMO (UID/AGR/00690/2019); L. Barros and M.I. Dias thank the national funding by FCT, P.I., through the institutional scientific employment program-contract for their contracts. The authors are also grateful to FEDER-Interreg España-Portugal programme for financial support through the project 0377_Iberphenol_6_E.Biblioteca Digital do IPBSouilem, FediaDias, Maria InêsBarros, LillianCalhelha, Ricardo C.Alves, Maria JoséHarzallah-Skhiri, FethiaFerreira, Isabel C.F.R.2018-01-19T10:00:00Z20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/20086engSouilem, Fedia; Dias, Maria Inês; Barros, Lillian; Calhelha, Ricardo C.; Alves, Maria José; Harzallah-Skhiri, Fethia; Ferreira, Isabel C.F.R. (2019). Phenolic profile and bioactive properties of carissa macrocarpa (Eckl.) A.DC.: An in vitro comparative study between leaves, stems, and flowers. Molecules. ISSN 1420-3049. 24, p. 1-1210.3390/molecules24091696info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:45:54Zoai:bibliotecadigital.ipb.pt:10198/20086Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:10:52.918550Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Phenolic profile and bioactive properties of carissa macrocarpa (Eckl.) A.DC.: An in vitro comparative study between leaves, stems, and flowers |
title |
Phenolic profile and bioactive properties of carissa macrocarpa (Eckl.) A.DC.: An in vitro comparative study between leaves, stems, and flowers |
spellingShingle |
Phenolic profile and bioactive properties of carissa macrocarpa (Eckl.) A.DC.: An in vitro comparative study between leaves, stems, and flowers Souilem, Fedia Bioactivities Carissa macrocarpa Pearson's correlation Phenolic profile Plant aerial parts |
title_short |
Phenolic profile and bioactive properties of carissa macrocarpa (Eckl.) A.DC.: An in vitro comparative study between leaves, stems, and flowers |
title_full |
Phenolic profile and bioactive properties of carissa macrocarpa (Eckl.) A.DC.: An in vitro comparative study between leaves, stems, and flowers |
title_fullStr |
Phenolic profile and bioactive properties of carissa macrocarpa (Eckl.) A.DC.: An in vitro comparative study between leaves, stems, and flowers |
title_full_unstemmed |
Phenolic profile and bioactive properties of carissa macrocarpa (Eckl.) A.DC.: An in vitro comparative study between leaves, stems, and flowers |
title_sort |
Phenolic profile and bioactive properties of carissa macrocarpa (Eckl.) A.DC.: An in vitro comparative study between leaves, stems, and flowers |
author |
Souilem, Fedia |
author_facet |
Souilem, Fedia Dias, Maria Inês Barros, Lillian Calhelha, Ricardo C. Alves, Maria José Harzallah-Skhiri, Fethia Ferreira, Isabel C.F.R. |
author_role |
author |
author2 |
Dias, Maria Inês Barros, Lillian Calhelha, Ricardo C. Alves, Maria José Harzallah-Skhiri, Fethia Ferreira, Isabel C.F.R. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Souilem, Fedia Dias, Maria Inês Barros, Lillian Calhelha, Ricardo C. Alves, Maria José Harzallah-Skhiri, Fethia Ferreira, Isabel C.F.R. |
dc.subject.por.fl_str_mv |
Bioactivities Carissa macrocarpa Pearson's correlation Phenolic profile Plant aerial parts |
topic |
Bioactivities Carissa macrocarpa Pearson's correlation Phenolic profile Plant aerial parts |
description |
The present work aimed to characterize leaves, stems, and flowers of Carissa macrocarpa (Eckl.) A.DC., by performing an analysis of the phenolic compounds by HPLC-DAD/ESI-MS, correlating them with bioactive properties, such as antioxidant, anti-inflammatory, cytotoxic, and antimicrobial activities. Thirty polyphenols were identified in the hydroethanolic extract, including phenolic acids, flavan-3-ols, and flavonol glycosides derivatives (which presented the highest number of identified compounds). However, flavan-3-ols showed the highest concentration in stems (mainly owing to the presence of dimers, trimmers, and tetramers of type B (epi)catechin). Leaves were distinguished by their high antioxidant and anti-inflammatory activities, as well as their bactericidal e ect against E. coli, while stems presented a higher cytotoxic activity and bactericidal e ect against Gram-positive bacteria. Moreover, a high correlation between the studied bioactivities and the presence of phenolic compounds was also verified. The obtained results bring added value to the studied plant species. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-19T10:00:00Z 2019 2019-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/20086 |
url |
http://hdl.handle.net/10198/20086 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Souilem, Fedia; Dias, Maria Inês; Barros, Lillian; Calhelha, Ricardo C.; Alves, Maria José; Harzallah-Skhiri, Fethia; Ferreira, Isabel C.F.R. (2019). Phenolic profile and bioactive properties of carissa macrocarpa (Eckl.) A.DC.: An in vitro comparative study between leaves, stems, and flowers. Molecules. ISSN 1420-3049. 24, p. 1-12 10.3390/molecules24091696 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799135375486091264 |