Within host selection for faster replicating bacterial symbionts

Detalhes bibliográficos
Autor(a) principal: Chrostek, Ewa
Data de Publicação: 2018
Outros Autores: Teixeira, Luis
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.7/824
Resumo: This deposit is composed by the main article plus the supplementary materials of the publication.
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spelling Within host selection for faster replicating bacterial symbiontsWolbachiaDrosophila melanogasterInvertebrate genomicsNatural selectionBacterial pathogensGene poolGene amplificationPolymerase chain reactionThis deposit is composed by the main article plus the supplementary materials of the publication.The supplementary materials are present in the publisher's page in the following link: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0191530#sec010Wolbachia is a widespread, intracellular symbiont of arthropods, able to induce reproductive distortions and antiviral protection in insects. Wolbachia can also be pathogenic, as is the case with wMelPop, a virulent variant of the endosymbiont of Drosophila melanogaster. An extensive genomic amplification of the 20kb region encompassing eight Wolbachia genes, called Octomom, is responsible for wMelPop virulence. The Octomom copy number in wMelPop can be highly variable between individual D. melanogaster flies, even when comparing siblings arising from a single female. Moreover, Octomom copy number can change rapidly between generations. These data suggest an intra-host variability in Octomom copy number between Wolbachia cells. Since wMelPop Wolbachia with different Octomom copy numbers grow at different rates, we hypothesized that selection could act on this intra-host variability. Here we tested if total Octomom copy number changes during the lifespan of individual Drosophila hosts, revealing selection for different Wolbachia populations. We performed a time course analysis of Octomom amplification in flies whose mothers were controlled for Octomom copy number. We show that despite the Octomom copy number being relatively stable it increases slightly throughout D. melanogaster adult life. This indicates that there is selection acting on the intra-host variation in the Octomom copy number over the lifespan of individual hosts. This within host selection for faster replicating bacterial symbionts may be in conflict with between host selection against highly pathogenic Wolbachia.Fundação para a Ciência e Tecnologia (www.fct.pt) grant PTDC/BEX-GMG/3128/2014; EMBO Long Term Fellowship: (EMBO ALTF 1497-2015); Marie Curie Actions; European Commission grants: (LTFCOFUND2013, GA-2013-609409).Public Library of ScienceARCAChrostek, EwaTeixeira, Luis2018-01-25T17:00:43Z2018-01-182018-01-18T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.7/824engChrostek E, Teixeira L (2018) Within host selection for faster replicating bacterial symbionts. PLoS ONE 13(1): e0191530. https://doi.org/10.1371/journal.pone.019153010.1371/journal.pone.0191530info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-11-29T14:35:14Zoai:arca.igc.gulbenkian.pt:10400.7/824Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:12:02.708637Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Within host selection for faster replicating bacterial symbionts
title Within host selection for faster replicating bacterial symbionts
spellingShingle Within host selection for faster replicating bacterial symbionts
Chrostek, Ewa
Wolbachia
Drosophila melanogaster
Invertebrate genomics
Natural selection
Bacterial pathogens
Gene pool
Gene amplification
Polymerase chain reaction
title_short Within host selection for faster replicating bacterial symbionts
title_full Within host selection for faster replicating bacterial symbionts
title_fullStr Within host selection for faster replicating bacterial symbionts
title_full_unstemmed Within host selection for faster replicating bacterial symbionts
title_sort Within host selection for faster replicating bacterial symbionts
author Chrostek, Ewa
author_facet Chrostek, Ewa
Teixeira, Luis
author_role author
author2 Teixeira, Luis
author2_role author
dc.contributor.none.fl_str_mv ARCA
dc.contributor.author.fl_str_mv Chrostek, Ewa
Teixeira, Luis
dc.subject.por.fl_str_mv Wolbachia
Drosophila melanogaster
Invertebrate genomics
Natural selection
Bacterial pathogens
Gene pool
Gene amplification
Polymerase chain reaction
topic Wolbachia
Drosophila melanogaster
Invertebrate genomics
Natural selection
Bacterial pathogens
Gene pool
Gene amplification
Polymerase chain reaction
description This deposit is composed by the main article plus the supplementary materials of the publication.
publishDate 2018
dc.date.none.fl_str_mv 2018-01-25T17:00:43Z
2018-01-18
2018-01-18T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.7/824
url http://hdl.handle.net/10400.7/824
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Chrostek E, Teixeira L (2018) Within host selection for faster replicating bacterial symbionts. PLoS ONE 13(1): e0191530. https://doi.org/10.1371/journal.pone.0191530
10.1371/journal.pone.0191530
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
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instacron_str RCAAP
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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