Development of Chitosan and Poly (Vinyl Alcohol) Blended scaffolds for cell culture using supercritical fluids technology

Detalhes bibliográficos
Autor(a) principal: Silva, Lígia Marina Clemente
Data de Publicação: 2008
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/1805
Resumo: Dissertação apresentada na Faculdade de Ciências e Tecnologia da Universidade Nova de Lisboa para obtenção do grau de Mestre em Mestrado Integrado em Engenharia Química e Bioquímica
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spelling Development of Chitosan and Poly (Vinyl Alcohol) Blended scaffolds for cell culture using supercritical fluids technologyDissertação apresentada na Faculdade de Ciências e Tecnologia da Universidade Nova de Lisboa para obtenção do grau de Mestre em Mestrado Integrado em Engenharia Química e BioquímicaIn this work new porous materials for biomedical applications were developed using supercritical fluids (SCF) technology. In this context, two types of porous structures constituted by blends of chitosan and poly (vinyl alcohol) (PVA) using different ratios were prepared: membranes and three dimensional (3D) scaffolds. Phase inversion method using supercritical carbon dioxide (scCO2) as non-solvent was used to prepare porous membranes. The characterization of these materials showed that the presence of PVA in the membranes composition causes a decrease in porosity values and contact angle and an increase on pores interconnectivity and swelling degree. The mechanical properties were also modified by the existence of PVA in the membranes composition, increasing the elongation capacity of these structures and decreasing the supported break stresses. The 3D-scaffolds were prepared using freeze-drying technique and were treated with glutaraldehyde using a CO2-assisted crosslinking process. The optimization of the crosslinking process comprised the study of different glutaraldehyde concentrations and operation time. Morphological characterization showed that the casting solution concentration was a parameter with no influence in the 3Dscaffolds porosity neither on the pores interconnectivity. However, the PVA content in these matrices and the temperature used in the freeze-drying process determined their morphology. These highly porous structures were also submitted to dynamic swelling tests proving to be pH-sensitive with the capacity of swelling and deswelling when submitted to pH variations. The best response was obtained for scaffolds crosslinked with 1% (v/v) of glutaraldehyde during 10 minutes. Membranes and 3D-scaffolds were also tested for in vitro biodegradation using lysozyme and for cytotoxicity, proving to have potential applications in tissue engineering and regenerative medicine.FCT - UNLRicardo, Ana AguiarRUNSilva, Lígia Marina Clemente2009-01-29T14:37:53Z20082008-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/1805enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:31:52Zoai:run.unl.pt:10362/1805Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:14:51.886679Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Development of Chitosan and Poly (Vinyl Alcohol) Blended scaffolds for cell culture using supercritical fluids technology
title Development of Chitosan and Poly (Vinyl Alcohol) Blended scaffolds for cell culture using supercritical fluids technology
spellingShingle Development of Chitosan and Poly (Vinyl Alcohol) Blended scaffolds for cell culture using supercritical fluids technology
Silva, Lígia Marina Clemente
title_short Development of Chitosan and Poly (Vinyl Alcohol) Blended scaffolds for cell culture using supercritical fluids technology
title_full Development of Chitosan and Poly (Vinyl Alcohol) Blended scaffolds for cell culture using supercritical fluids technology
title_fullStr Development of Chitosan and Poly (Vinyl Alcohol) Blended scaffolds for cell culture using supercritical fluids technology
title_full_unstemmed Development of Chitosan and Poly (Vinyl Alcohol) Blended scaffolds for cell culture using supercritical fluids technology
title_sort Development of Chitosan and Poly (Vinyl Alcohol) Blended scaffolds for cell culture using supercritical fluids technology
author Silva, Lígia Marina Clemente
author_facet Silva, Lígia Marina Clemente
author_role author
dc.contributor.none.fl_str_mv Ricardo, Ana Aguiar
RUN
dc.contributor.author.fl_str_mv Silva, Lígia Marina Clemente
description Dissertação apresentada na Faculdade de Ciências e Tecnologia da Universidade Nova de Lisboa para obtenção do grau de Mestre em Mestrado Integrado em Engenharia Química e Bioquímica
publishDate 2008
dc.date.none.fl_str_mv 2008
2008-01-01T00:00:00Z
2009-01-29T14:37:53Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/1805
url http://hdl.handle.net/10362/1805
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv FCT - UNL
publisher.none.fl_str_mv FCT - UNL
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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