Bio-inspired polymeric iron-doped hydroxyapatite microspheres as a tunable carrier of rhBMP-2
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.8/8265 |
Resumo: | Hybrid superparamagnetic microspheres with bone-like composition, previously developed by a bio-inspired assembling/mineralization process, are evaluated for their ability to uptake and deliver recombinant human bone morphogenetic protein-2 (rhBMP-2) in therapeutically-relevant doses along with prolonged release pro- files. The comparison with hybrid non-magnetic and with non-mineralized microspheres highlights the role of nanocrystalline, nanosize mineral phases when they exhibit surface charged groups enabling the chemical linking with the growth factor and thus moderating the release kinetics. All the microspheres show excellent osteogenic ability with human mesenchymal stem cells whereas the hybrid mineralized ones show a slow and sustained release of rhBMP-2 along 14 days of soaking into cell culture medium with substantially bioactive effect, as reported by assay with C2C12 BRE-Luc cell line. It is also shown that the release extent can be modulated by the application of pulsed electromagnetic field, thus showing the potential of remote controlling the bioactivity of the new micro-devices which is promising for future application of hybrid biomimetic mi- crospheres in precisely designed and personalized therapies. |
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Bio-inspired polymeric iron-doped hydroxyapatite microspheres as a tunable carrier of rhBMP-2Bone regenerationPulsed electromagnetic fieldSustained releaseOsteogenesisHybrid superparamagnetic microspheresrhBMP-2Hybrid superparamagnetic microspheres with bone-like composition, previously developed by a bio-inspired assembling/mineralization process, are evaluated for their ability to uptake and deliver recombinant human bone morphogenetic protein-2 (rhBMP-2) in therapeutically-relevant doses along with prolonged release pro- files. The comparison with hybrid non-magnetic and with non-mineralized microspheres highlights the role of nanocrystalline, nanosize mineral phases when they exhibit surface charged groups enabling the chemical linking with the growth factor and thus moderating the release kinetics. All the microspheres show excellent osteogenic ability with human mesenchymal stem cells whereas the hybrid mineralized ones show a slow and sustained release of rhBMP-2 along 14 days of soaking into cell culture medium with substantially bioactive effect, as reported by assay with C2C12 BRE-Luc cell line. It is also shown that the release extent can be modulated by the application of pulsed electromagnetic field, thus showing the potential of remote controlling the bioactivity of the new micro-devices which is promising for future application of hybrid biomimetic mi- crospheres in precisely designed and personalized therapies.ScienceDirectIC-OnlinePatrício, Tatiana Marisa FernandesMumcuoglu, DidemMontesi, MonicaPanseri, SilviaWitte-Bouma, JannekeGarcia, Shorouk FahmySandri, MonicaTampieri, AnnaFarrell, EricSprio, Simone2023-03-20T15:07:12Z2020-08-222020-08-22T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.8/8265engTatiana M. Fernandes Patrício, Didem Mumcuoglu, Monica Montesi, Silvia Panseri, Janneke Witte-Bouma, Shorouk Fahmy Garcia, Monica Sandri, Anna Tampieri, Eric Farrell, Simone Sprio, Bio-inspired polymeric iron-doped hydroxyapatite microspheres as a tunable carrier of rhBMP-2, Materials Science and Engineering: C, Volume 119, 2021, 111410, ISSN 0928-4931, https://doi.org/10.1016/j.msec.2020.1114101873-019110.1016/j.msec.2020.111410info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-17T15:56:58Zoai:iconline.ipleiria.pt:10400.8/8265Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:51:01.943559Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Bio-inspired polymeric iron-doped hydroxyapatite microspheres as a tunable carrier of rhBMP-2 |
title |
Bio-inspired polymeric iron-doped hydroxyapatite microspheres as a tunable carrier of rhBMP-2 |
spellingShingle |
Bio-inspired polymeric iron-doped hydroxyapatite microspheres as a tunable carrier of rhBMP-2 Patrício, Tatiana Marisa Fernandes Bone regeneration Pulsed electromagnetic field Sustained release Osteogenesis Hybrid superparamagnetic microspheres rhBMP-2 |
title_short |
Bio-inspired polymeric iron-doped hydroxyapatite microspheres as a tunable carrier of rhBMP-2 |
title_full |
Bio-inspired polymeric iron-doped hydroxyapatite microspheres as a tunable carrier of rhBMP-2 |
title_fullStr |
Bio-inspired polymeric iron-doped hydroxyapatite microspheres as a tunable carrier of rhBMP-2 |
title_full_unstemmed |
Bio-inspired polymeric iron-doped hydroxyapatite microspheres as a tunable carrier of rhBMP-2 |
title_sort |
Bio-inspired polymeric iron-doped hydroxyapatite microspheres as a tunable carrier of rhBMP-2 |
author |
Patrício, Tatiana Marisa Fernandes |
author_facet |
Patrício, Tatiana Marisa Fernandes Mumcuoglu, Didem Montesi, Monica Panseri, Silvia Witte-Bouma, Janneke Garcia, Shorouk Fahmy Sandri, Monica Tampieri, Anna Farrell, Eric Sprio, Simone |
author_role |
author |
author2 |
Mumcuoglu, Didem Montesi, Monica Panseri, Silvia Witte-Bouma, Janneke Garcia, Shorouk Fahmy Sandri, Monica Tampieri, Anna Farrell, Eric Sprio, Simone |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
IC-Online |
dc.contributor.author.fl_str_mv |
Patrício, Tatiana Marisa Fernandes Mumcuoglu, Didem Montesi, Monica Panseri, Silvia Witte-Bouma, Janneke Garcia, Shorouk Fahmy Sandri, Monica Tampieri, Anna Farrell, Eric Sprio, Simone |
dc.subject.por.fl_str_mv |
Bone regeneration Pulsed electromagnetic field Sustained release Osteogenesis Hybrid superparamagnetic microspheres rhBMP-2 |
topic |
Bone regeneration Pulsed electromagnetic field Sustained release Osteogenesis Hybrid superparamagnetic microspheres rhBMP-2 |
description |
Hybrid superparamagnetic microspheres with bone-like composition, previously developed by a bio-inspired assembling/mineralization process, are evaluated for their ability to uptake and deliver recombinant human bone morphogenetic protein-2 (rhBMP-2) in therapeutically-relevant doses along with prolonged release pro- files. The comparison with hybrid non-magnetic and with non-mineralized microspheres highlights the role of nanocrystalline, nanosize mineral phases when they exhibit surface charged groups enabling the chemical linking with the growth factor and thus moderating the release kinetics. All the microspheres show excellent osteogenic ability with human mesenchymal stem cells whereas the hybrid mineralized ones show a slow and sustained release of rhBMP-2 along 14 days of soaking into cell culture medium with substantially bioactive effect, as reported by assay with C2C12 BRE-Luc cell line. It is also shown that the release extent can be modulated by the application of pulsed electromagnetic field, thus showing the potential of remote controlling the bioactivity of the new micro-devices which is promising for future application of hybrid biomimetic mi- crospheres in precisely designed and personalized therapies. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-08-22 2020-08-22T00:00:00Z 2023-03-20T15:07:12Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.8/8265 |
url |
http://hdl.handle.net/10400.8/8265 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Tatiana M. Fernandes Patrício, Didem Mumcuoglu, Monica Montesi, Silvia Panseri, Janneke Witte-Bouma, Shorouk Fahmy Garcia, Monica Sandri, Anna Tampieri, Eric Farrell, Simone Sprio, Bio-inspired polymeric iron-doped hydroxyapatite microspheres as a tunable carrier of rhBMP-2, Materials Science and Engineering: C, Volume 119, 2021, 111410, ISSN 0928-4931, https://doi.org/10.1016/j.msec.2020.111410 1873-0191 10.1016/j.msec.2020.111410 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
ScienceDirect |
publisher.none.fl_str_mv |
ScienceDirect |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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