Nitrite reduction by xanthine oxidase family enzymes: a new class of nitrite reductases

Detalhes bibliográficos
Autor(a) principal: Maia, Luisa B.
Data de Publicação: 2011
Outros Autores: Moura, José J. G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/8621
Resumo: J Biol Inorg Chem (2011) 16:443–460 DOI 10.1007/s00775-010-0741-z
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spelling Nitrite reduction by xanthine oxidase family enzymes: a new class of nitrite reductasesNitrite reductionNitric oxide formationMolybdenumXanthine oxidaseAldehyde oxidoreductaseJ Biol Inorg Chem (2011) 16:443–460 DOI 10.1007/s00775-010-0741-zMammalian xanthine oxidase (XO) and Desulfovibrio gigas aldehyde oxidoreductase (AOR) are members of the XO family of mononuclear molybdoenzymes that catalyse the oxidative hydroxylation of a wide range of aldehydes and heterocyclic compounds. Much less known is the XO ability to catalyse the nitrite reduction to nitric oxide radical (NO). To assess the competence of other XO family enzymes to catalyse the nitrite reduction and to shed some light onto the molecular mechanism of this reaction, we characterised the anaerobic XO- and AORcatalysed nitrite reduction. The identification of NO as the reaction product was done with a NO-selective electrode and by electron paramagnetic resonance (EPR) spectroscopy. The steady-state kinetic characterisation corroborated the XO-catalysed nitrite reduction and demonstrated, for the first time, that the prokaryotic AOR does catalyse the nitrite reduction to NO, in the presence of any electron donor to the enzyme, substrate (aldehyde) or not (dithionite). Nitrite binding and reduction was shown by EPR spectroscopy to occur on a reduced molybdenum centre. A molecular mechanism of AOR- and XO-catalysed nitrite reduction is discussed, in which the higher oxidation states of molybdenum seem to be involved in oxygen-atom insertion, whereas the lower oxidation states would favour oxygenatom abstraction. Our results define a new catalytic performance for AOR—the nitrite reduction—and propose a new class of molybdenum-containing nitrite reductases. Keywords Nitrite reduction Nitric oxide formation Molybdenum Xanthine oxidase Aldehyde oxidoreductase Abbreviations AOR Aldehyde oxidoreductase DMSOR Dimethylsulfoxide reductase EPR Electron paramagnetic resonance Fe/S Iron–sulfur centre Fe/S–NO Dinitrosyl–iron–sulfur complex (MGD)2–Fe Ferrous complex of di(N-methyl-Dglucamine dithiocarbamate)(MGD)2–Fe–NO Mononitrosyl–iron complex Mo-enzymes Pterin–molybdenum-containing enzymes NaR Nitrate reductases NO Nitric oxide radical SO Sulfite oxidase XO Xanthine oxidase Introduction Molybdenum is present in a wide variety of enzymes, in both prokaryotes and eukaryotes, where it performs catalyticSpringerRUNMaia, Luisa B.Moura, José J. G.2013-01-30T12:01:11Z20112011-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/8621enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:41:26Zoai:run.unl.pt:10362/8621Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:18:19.549875Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Nitrite reduction by xanthine oxidase family enzymes: a new class of nitrite reductases
title Nitrite reduction by xanthine oxidase family enzymes: a new class of nitrite reductases
spellingShingle Nitrite reduction by xanthine oxidase family enzymes: a new class of nitrite reductases
Maia, Luisa B.
Nitrite reduction
Nitric oxide formation
Molybdenum
Xanthine oxidase
Aldehyde oxidoreductase
title_short Nitrite reduction by xanthine oxidase family enzymes: a new class of nitrite reductases
title_full Nitrite reduction by xanthine oxidase family enzymes: a new class of nitrite reductases
title_fullStr Nitrite reduction by xanthine oxidase family enzymes: a new class of nitrite reductases
title_full_unstemmed Nitrite reduction by xanthine oxidase family enzymes: a new class of nitrite reductases
title_sort Nitrite reduction by xanthine oxidase family enzymes: a new class of nitrite reductases
author Maia, Luisa B.
author_facet Maia, Luisa B.
Moura, José J. G.
author_role author
author2 Moura, José J. G.
author2_role author
dc.contributor.none.fl_str_mv RUN
dc.contributor.author.fl_str_mv Maia, Luisa B.
Moura, José J. G.
dc.subject.por.fl_str_mv Nitrite reduction
Nitric oxide formation
Molybdenum
Xanthine oxidase
Aldehyde oxidoreductase
topic Nitrite reduction
Nitric oxide formation
Molybdenum
Xanthine oxidase
Aldehyde oxidoreductase
description J Biol Inorg Chem (2011) 16:443–460 DOI 10.1007/s00775-010-0741-z
publishDate 2011
dc.date.none.fl_str_mv 2011
2011-01-01T00:00:00Z
2013-01-30T12:01:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/8621
url http://hdl.handle.net/10362/8621
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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