Study of biomarkers to evaluate the protective effects of antioxidants against manganese neurotoxicity

Detalhes bibliográficos
Autor(a) principal: Santos, Maria Lopes
Data de Publicação: 2008
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/1261
Resumo: Tese de mestrado, Biologia (Biologia Humana e Ambiente), 2008, Universidade de Lisboa, Faculdade de Ciências
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spelling Study of biomarkers to evaluate the protective effects of antioxidants against manganese neurotoxicityBioquímicaMarcadores biológicosManganismoNeurotoxicologiaTeses de mestradoTese de mestrado, Biologia (Biologia Humana e Ambiente), 2008, Universidade de Lisboa, Faculdade de CiênciasManganese (Mn) an essential nutrient for several animal species is required for normal aminoacid, lipid, protein and carbohydrate metabolism. However, chronic exposure to this metal can originate accumulation in the brain, leading to adverse health effects, particularly in the central nervous system. Although the exact mechanism of Mn toxicity is not completely established, several studies indicate that oxidative stress plays a major role in the Mn-induced toxicity process. To avoid overexposure and the consequent neurotoxic effects, people chronically exposed must be monitorized recorring to adequate biomarkers. The aim of this study was to examine the correlation between exposure and effect biomarkers and the possible protective effect of ebselen, a compound with antioxidant properties, against Mn toxicity. In an in vivo assay rats were exposed to several doses of Mn (4 and 8 doses) by intraperitoneal injection of manganese chloride (10.0 mg/kg). The exposure to Mn was evaluated by measuring Mn levels in the brain and blood. Mn levels in brain were increased and significantly different from control (p<0.0001, for both doses). On the other hand, blood levels failed to evidence the accumulation of Mn in the brain in a dose-dependent manner, with only 8 doses group showing significant differences (p<0.0001). The levels of reduced glutathione (GSH) in the brain were assessed as an indirect indicator of oxidative stress and prolactin levels in serum were analyzed. GSH levels were significantly decreased in 8 doses treated animals (p<0.0001). Prolactin was shown to be a valuable indicator of the neurotoxic effects of Mn, since significant and dose-dependent increase was found for both treated groups (p<0.0001). In addition a decrease in motor activity was observed in the 8 doses group. Finally, ebselen evidenced a protective effect against Mn accumulation in the brain, for the 8 doses group (p<0.0001). Yet, it doesn't seem to protect against oxidative stress since decreased values of GSH were observedSantos, Ana Paula Marreilha dosDias, Deodália Maria Antunes, 1952-Repositório da Universidade de LisboaSantos, Maria Lopes2010-07-27T08:56:50Z20082008-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdftext/xmlhttp://hdl.handle.net/10451/1261enghttp://catalogo.ul.pt/F/?func=item-global&doc_library=ULB01&type=03&doc_number=000561592info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T15:40:40Zoai:repositorio.ul.pt:10451/1261Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:27:54.752434Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Study of biomarkers to evaluate the protective effects of antioxidants against manganese neurotoxicity
title Study of biomarkers to evaluate the protective effects of antioxidants against manganese neurotoxicity
spellingShingle Study of biomarkers to evaluate the protective effects of antioxidants against manganese neurotoxicity
Santos, Maria Lopes
Bioquímica
Marcadores biológicos
Manganismo
Neurotoxicologia
Teses de mestrado
title_short Study of biomarkers to evaluate the protective effects of antioxidants against manganese neurotoxicity
title_full Study of biomarkers to evaluate the protective effects of antioxidants against manganese neurotoxicity
title_fullStr Study of biomarkers to evaluate the protective effects of antioxidants against manganese neurotoxicity
title_full_unstemmed Study of biomarkers to evaluate the protective effects of antioxidants against manganese neurotoxicity
title_sort Study of biomarkers to evaluate the protective effects of antioxidants against manganese neurotoxicity
author Santos, Maria Lopes
author_facet Santos, Maria Lopes
author_role author
dc.contributor.none.fl_str_mv Santos, Ana Paula Marreilha dos
Dias, Deodália Maria Antunes, 1952-
Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Santos, Maria Lopes
dc.subject.por.fl_str_mv Bioquímica
Marcadores biológicos
Manganismo
Neurotoxicologia
Teses de mestrado
topic Bioquímica
Marcadores biológicos
Manganismo
Neurotoxicologia
Teses de mestrado
description Tese de mestrado, Biologia (Biologia Humana e Ambiente), 2008, Universidade de Lisboa, Faculdade de Ciências
publishDate 2008
dc.date.none.fl_str_mv 2008
2008-01-01T00:00:00Z
2010-07-27T08:56:50Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/1261
url http://hdl.handle.net/10451/1261
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv http://catalogo.ul.pt/F/?func=item-global&doc_library=ULB01&type=03&doc_number=000561592
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
text/xml
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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