A Conserved GPG-Motif in the HIV-1 Nef Core Is required for principal nef-activities

Detalhes bibliográficos
Autor(a) principal: Martínez-Bonet, Marta
Data de Publicação: 2015
Outros Autores: Palladino, Claudia, Briz, Veronica, Rudolph, Jochen M., Fackler, Oliver T., Relloso, Miguel, Muñoz-Fernandez, Maria Angeles, Madrid, Ricardo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/61039
Resumo: © 2015 Martínez-Bonet et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
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spelling A Conserved GPG-Motif in the HIV-1 Nef Core Is required for principal nef-activities© 2015 Martínez-Bonet et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are creditedTo find out new determinants required for Nef activity we performed a functional alanine scanning analysis along a discrete but highly conserved region at the core of HIV-1 Nef. We identified the GPG-motif, located at the 121–137 region of HIV-1 NL4.3 Nef, as a novel protein signature strictly required for the p56Lck dependent Nef-induced CD4-downregulation in T-cells. Since the Nef-GPG motif was dispensable for CD4-downregulation in HeLa-CD4 cells, Nef/AP-1 interaction and Nef-dependent effects on Tf-R trafficking, the observed effects on CD4 downregulation cannot be attributed to structure constraints or to alterations on general protein trafficking. Besides, we found that the GPG-motif was also required for Nef-dependent inhibition of ring actin re-organization upon TCR triggering and MHCI downregulation, suggesting that the GPG-motif could actively cooperate with the Nef PxxP motif for these HIV-1 Nef-related effects. Finally, we observed that the Nef-GPG motif was required for optimal infectivity of those viruses produced in T-cells. According to these findings, we propose the conserved GPG-motif in HIV-1 Nef as functional region required for HIV-1 infectivity and therefore with a potential interest for the interference of Nef activity during HIV-1 infection.This work was supported by the Ramon y Cajal research program (MICIIN-RYC-2005-002174; http://www.mineco.gob.es/portal/site/mineco/idi), Fondo de Investigación Sanitaria en España (FIS-PS09/01386; http://www.isciii.es/) to RM, and Comunidad de Madrid (S-2010/BMD-2332; http://www.madrimasd.org/) to MAM-F, and the Deutsche Forschungsgemeinschaft (TRR83, project 15 to OTF). MM-B, CP and VB hold a fellowship from the Fondo de Investigación Sanitaria en España (FIS, http://www.isciii.es/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.PLoSRepositório da Universidade de LisboaMartínez-Bonet, MartaPalladino, ClaudiaBriz, VeronicaRudolph, Jochen M.Fackler, Oliver T.Relloso, MiguelMuñoz-Fernandez, Maria AngelesMadrid, Ricardo2023-11-30T15:33:01Z20152023-09-15T14:40:02Z2015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/61039engMartínez-Bonet M, Palladino C, Briz V, Rudolph JM, Fackler OT, Relloso M, et al. A conserved gpg-motif in the hiv-1 nef core is required for principal nef-activities. Wu Y, editor. PLoS ONE. 2015;10(12):e0145239. Disponível em: https://dx.plos.org/10.1371/journal.pone.0145239cv-prod-118552810.1371/journal.pone.01452392-s2.0-84956906858info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-12-04T01:18:22Zoai:repositorio.ul.pt:10451/61039Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:40:48.095633Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A Conserved GPG-Motif in the HIV-1 Nef Core Is required for principal nef-activities
title A Conserved GPG-Motif in the HIV-1 Nef Core Is required for principal nef-activities
spellingShingle A Conserved GPG-Motif in the HIV-1 Nef Core Is required for principal nef-activities
Martínez-Bonet, Marta
title_short A Conserved GPG-Motif in the HIV-1 Nef Core Is required for principal nef-activities
title_full A Conserved GPG-Motif in the HIV-1 Nef Core Is required for principal nef-activities
title_fullStr A Conserved GPG-Motif in the HIV-1 Nef Core Is required for principal nef-activities
title_full_unstemmed A Conserved GPG-Motif in the HIV-1 Nef Core Is required for principal nef-activities
title_sort A Conserved GPG-Motif in the HIV-1 Nef Core Is required for principal nef-activities
author Martínez-Bonet, Marta
author_facet Martínez-Bonet, Marta
Palladino, Claudia
Briz, Veronica
Rudolph, Jochen M.
Fackler, Oliver T.
Relloso, Miguel
Muñoz-Fernandez, Maria Angeles
Madrid, Ricardo
author_role author
author2 Palladino, Claudia
Briz, Veronica
Rudolph, Jochen M.
Fackler, Oliver T.
Relloso, Miguel
Muñoz-Fernandez, Maria Angeles
Madrid, Ricardo
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Martínez-Bonet, Marta
Palladino, Claudia
Briz, Veronica
Rudolph, Jochen M.
Fackler, Oliver T.
Relloso, Miguel
Muñoz-Fernandez, Maria Angeles
Madrid, Ricardo
description © 2015 Martínez-Bonet et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-01-01T00:00:00Z
2023-11-30T15:33:01Z
2023-09-15T14:40:02Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/61039
url http://hdl.handle.net/10451/61039
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Martínez-Bonet M, Palladino C, Briz V, Rudolph JM, Fackler OT, Relloso M, et al. A conserved gpg-motif in the hiv-1 nef core is required for principal nef-activities. Wu Y, editor. PLoS ONE. 2015;10(12):e0145239. Disponível em: https://dx.plos.org/10.1371/journal.pone.0145239
cv-prod-1185528
10.1371/journal.pone.0145239
2-s2.0-84956906858
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv PLoS
publisher.none.fl_str_mv PLoS
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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