Estimation of kinetic parameters related to biochemical interactions between hydrogen peroxide and signal transduction proteins

Detalhes bibliográficos
Autor(a) principal: Brito, Paula
Data de Publicação: 2014
Outros Autores: Antunes, Fernando
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/51349
Resumo: Copyright © 2014 Brito and Antunes. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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spelling Estimation of kinetic parameters related to biochemical interactions between hydrogen peroxide and signal transduction proteinsPTP1BSHP-2KineticsProtein tyrosine phosphatasesRate constantRedox regulationRedox signalingCopyright © 2014 Brito and Antunes. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.The lack of kinetic data concerning the biological effects of reactive oxygen species is slowing down the development of the field of redox signaling. Herein, we deduced and applied equations to estimate kinetic parameters from typical redox signaling experiments. H2O2-sensing mediated by the oxidation of a protein target and the switch-off of this sensor, by being converted back to its reduced form, are the two processes for which kinetic parameters are determined. The experimental data required to apply the equations deduced is the fraction of the H2O2 sensor protein in the reduced or in the oxidized state measured in intact cells or living tissues after exposure to either endogenous or added H2O2. Either non-linear fittings that do not need transformation of the experimental data or linearized plots in which deviations from the equations are easily observed can be used. The equations were shown to be valid by fitting to them virtual time courses simulated with a kinetic model. The good agreement between the kinetic parameters estimated in these fittings and those used to simulate the virtual time courses supported the accuracy of the kinetic equations deduced. Finally, equations were successfully tested with real data taken from published experiments that describe redox signaling mediated by the oxidation of two protein tyrosine phosphatases, PTP1B and SHP-2, which are two of the few H2O2-sensing proteins with known kinetic parameters. Whereas for PTP1B estimated kinetic parameters fitted in general the present knowledge, for SHP-2 results obtained suggest that reactivity toward H2O2 as well as the rate of SHP-2 regeneration back to its reduced form are higher than previously thought. In conclusion, valuable quantitative kinetic data can be estimated from typical redox signaling experiments, thus improving our understanding about the complex processes that underlie the interplay between oxidative stress and redox signaling responses.Supported by Fundação para a Ciência e a Tecnologia (FCT), Portugal (PEst-OE/QUI/UI0612/2013 and VIH/SAU/0020/2011).FrontiersRepositório da Universidade de LisboaBrito, PaulaAntunes, Fernando2022-02-16T14:34:46Z20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/51349engFront Chem. 2014 Oct 2;2:8210.3389/fchem.2014.000822296-2646info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:55:59Zoai:repositorio.ul.pt:10451/51349Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:02:38.945987Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Estimation of kinetic parameters related to biochemical interactions between hydrogen peroxide and signal transduction proteins
title Estimation of kinetic parameters related to biochemical interactions between hydrogen peroxide and signal transduction proteins
spellingShingle Estimation of kinetic parameters related to biochemical interactions between hydrogen peroxide and signal transduction proteins
Brito, Paula
PTP1B
SHP-2
Kinetics
Protein tyrosine phosphatases
Rate constant
Redox regulation
Redox signaling
title_short Estimation of kinetic parameters related to biochemical interactions between hydrogen peroxide and signal transduction proteins
title_full Estimation of kinetic parameters related to biochemical interactions between hydrogen peroxide and signal transduction proteins
title_fullStr Estimation of kinetic parameters related to biochemical interactions between hydrogen peroxide and signal transduction proteins
title_full_unstemmed Estimation of kinetic parameters related to biochemical interactions between hydrogen peroxide and signal transduction proteins
title_sort Estimation of kinetic parameters related to biochemical interactions between hydrogen peroxide and signal transduction proteins
author Brito, Paula
author_facet Brito, Paula
Antunes, Fernando
author_role author
author2 Antunes, Fernando
author2_role author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Brito, Paula
Antunes, Fernando
dc.subject.por.fl_str_mv PTP1B
SHP-2
Kinetics
Protein tyrosine phosphatases
Rate constant
Redox regulation
Redox signaling
topic PTP1B
SHP-2
Kinetics
Protein tyrosine phosphatases
Rate constant
Redox regulation
Redox signaling
description Copyright © 2014 Brito and Antunes. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
publishDate 2014
dc.date.none.fl_str_mv 2014
2014-01-01T00:00:00Z
2022-02-16T14:34:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/51349
url http://hdl.handle.net/10451/51349
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Front Chem. 2014 Oct 2;2:82
10.3389/fchem.2014.00082
2296-2646
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers
publisher.none.fl_str_mv Frontiers
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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