Human-derived NLS enhance the gene transfer efficiency of chitosan

Detalhes bibliográficos
Autor(a) principal: Bitoque, Diogo B
Data de Publicação: 2020
Outros Autores: Morais, Joana, Oliveira, Ana V, Sequeira, Raquel L, Calado, Sofia M, Fortunato, Tiago M, Simão, Sonia, Rosa da Costa, Ana M, Silva, Gabriela A
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/109485
Resumo: The authors acknowledge Dr. Jean Bennett (University of Pennsylvania, USA) for kindly providing the GFP plasmid and Dr. Lyn Schedlich and Sue Firth for kindly providing the NLS plasmids. The authors also acknowledge the financial support of Fundação para a Ciência e Tecnologia (PTDC/SAL -BEB/098475/2008 to Gabriela A. Silva, PD/BD/52424/2013 individual fellowship to Diogo Bitoque, SFRH/BD/70318/2010 individual fellowship to Ana V. Oliveira, SFRH/BD/76873/2011 individual fellowship to Sofia Calado, SFRH/BPD/78404/2011 individual fellowship to Sónia Simão, IBB/LA under the project PEst- OE/EQB/LA0023/2013, and PEst-OE_QUI_UI4023_2011) and the Marie Curie Reintegration Grant (PIRG-GA-2009-249314 to Gabriela A. Silva) under the FP7 program. iNOVA4Health - UID/Multi/04462/2013, a program financially supported by Fundação para a Ciência e Tecnologia / Ministério da Educação e Ciência, through national funds and co-funded by FEDER under the PT2020 Partnership Agreement is also acknowledged.
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spelling Human-derived NLS enhance the gene transfer efficiency of chitosangene therapychitosannuclear localization signalsIGFBPHEK293T cellsBiotechnologyThe authors acknowledge Dr. Jean Bennett (University of Pennsylvania, USA) for kindly providing the GFP plasmid and Dr. Lyn Schedlich and Sue Firth for kindly providing the NLS plasmids. The authors also acknowledge the financial support of Fundação para a Ciência e Tecnologia (PTDC/SAL -BEB/098475/2008 to Gabriela A. Silva, PD/BD/52424/2013 individual fellowship to Diogo Bitoque, SFRH/BD/70318/2010 individual fellowship to Ana V. Oliveira, SFRH/BD/76873/2011 individual fellowship to Sofia Calado, SFRH/BPD/78404/2011 individual fellowship to Sónia Simão, IBB/LA under the project PEst- OE/EQB/LA0023/2013, and PEst-OE_QUI_UI4023_2011) and the Marie Curie Reintegration Grant (PIRG-GA-2009-249314 to Gabriela A. Silva) under the FP7 program. iNOVA4Health - UID/Multi/04462/2013, a program financially supported by Fundação para a Ciência e Tecnologia / Ministério da Educação e Ciência, through national funds and co-funded by FEDER under the PT2020 Partnership Agreement is also acknowledged.Nuclear import is considered one of the major limitations for non-viral gene delivery systems and the incorporation of nuclear localization signals (NLS) that mediate nuclear intake can be used as a strategy to enhance internalization of exogenous DNA. In this work, human-derived endogenous NLS peptides based on Insulin Growth Factor Binding Proteins (IGFBP), namely IGFBP-3 and IGFBP-5, were tested for their ability to improve nuclear translocation of genetic material by non-viral vectors. Several strategies were tested to determine their effect on chitosan mediated transfection efficiency: co-administration with polyplexes, co-complexation at the time of polyplex formation, and covalent ligation to chitosan. Our results show that co-complexation and covalent ligation of the NLS peptide derived from IGFBP-3 to chitosan polyplexes yields a 2-fold increase in transfection efficiency, which was not observed for NLS peptide derived from IGFBP-5. These results indicate that the integration of IGFBP-NLS-3 peptides into polyplexes has potential as a strategy to enhance the efficiency of non-viral vectors.Centro de Estudos de Doenças Crónicas (CEDOC)NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNBitoque, Diogo BMorais, JoanaOliveira, Ana VSequeira, Raquel LCalado, Sofia MFortunato, Tiago MSimão, SoniaRosa da Costa, Ana MSilva, Gabriela A2020-12-30T05:04:18Z2021-012021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/109485eng0144-8463PURE: 26861895https://doi.org/10.1042/BSR20201026info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-22T17:49:37Zoai:run.unl.pt:10362/109485Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-22T17:49:37Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Human-derived NLS enhance the gene transfer efficiency of chitosan
title Human-derived NLS enhance the gene transfer efficiency of chitosan
spellingShingle Human-derived NLS enhance the gene transfer efficiency of chitosan
Bitoque, Diogo B
gene therapy
chitosan
nuclear localization signals
IGFBP
HEK293T cells
Biotechnology
title_short Human-derived NLS enhance the gene transfer efficiency of chitosan
title_full Human-derived NLS enhance the gene transfer efficiency of chitosan
title_fullStr Human-derived NLS enhance the gene transfer efficiency of chitosan
title_full_unstemmed Human-derived NLS enhance the gene transfer efficiency of chitosan
title_sort Human-derived NLS enhance the gene transfer efficiency of chitosan
author Bitoque, Diogo B
author_facet Bitoque, Diogo B
Morais, Joana
Oliveira, Ana V
Sequeira, Raquel L
Calado, Sofia M
Fortunato, Tiago M
Simão, Sonia
Rosa da Costa, Ana M
Silva, Gabriela A
author_role author
author2 Morais, Joana
Oliveira, Ana V
Sequeira, Raquel L
Calado, Sofia M
Fortunato, Tiago M
Simão, Sonia
Rosa da Costa, Ana M
Silva, Gabriela A
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Centro de Estudos de Doenças Crónicas (CEDOC)
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
RUN
dc.contributor.author.fl_str_mv Bitoque, Diogo B
Morais, Joana
Oliveira, Ana V
Sequeira, Raquel L
Calado, Sofia M
Fortunato, Tiago M
Simão, Sonia
Rosa da Costa, Ana M
Silva, Gabriela A
dc.subject.por.fl_str_mv gene therapy
chitosan
nuclear localization signals
IGFBP
HEK293T cells
Biotechnology
topic gene therapy
chitosan
nuclear localization signals
IGFBP
HEK293T cells
Biotechnology
description The authors acknowledge Dr. Jean Bennett (University of Pennsylvania, USA) for kindly providing the GFP plasmid and Dr. Lyn Schedlich and Sue Firth for kindly providing the NLS plasmids. The authors also acknowledge the financial support of Fundação para a Ciência e Tecnologia (PTDC/SAL -BEB/098475/2008 to Gabriela A. Silva, PD/BD/52424/2013 individual fellowship to Diogo Bitoque, SFRH/BD/70318/2010 individual fellowship to Ana V. Oliveira, SFRH/BD/76873/2011 individual fellowship to Sofia Calado, SFRH/BPD/78404/2011 individual fellowship to Sónia Simão, IBB/LA under the project PEst- OE/EQB/LA0023/2013, and PEst-OE_QUI_UI4023_2011) and the Marie Curie Reintegration Grant (PIRG-GA-2009-249314 to Gabriela A. Silva) under the FP7 program. iNOVA4Health - UID/Multi/04462/2013, a program financially supported by Fundação para a Ciência e Tecnologia / Ministério da Educação e Ciência, through national funds and co-funded by FEDER under the PT2020 Partnership Agreement is also acknowledged.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-30T05:04:18Z
2021-01
2021-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/109485
url http://hdl.handle.net/10362/109485
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0144-8463
PURE: 26861895
https://doi.org/10.1042/BSR20201026
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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