Cryptic Prophages Contribution for Campylobacter jejuni and Campylobacter coli Introgression

Detalhes bibliográficos
Autor(a) principal: Tanoeiro, Luís
Data de Publicação: 2022
Outros Autores: Oleastro, Mónica, Nunes, Alexandra, Marques, Andreia T., Duarte, Sílvia Vaz, Gomes, João Paulo, Matos, António Pedro Alves, Vítor, Jorge M.B., Vale, Filipa F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/8484
Resumo: This article belongs to the Special Issue Bacteriophage Genomics.
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spelling Cryptic Prophages Contribution for Campylobacter jejuni and Campylobacter coli IntrogressionCampylobacterBacteriophageHost RangeIntrogressionInfecções GastrointestinaisThis article belongs to the Special Issue Bacteriophage Genomics.Campylobacter coli and C. jejuni, the causing agents of campylobacteriosis, are described to be undergoing introgression events, i.e., the transference of genetic material between different species, with some isolates sharing almost a quarter of its genome. The participation of phages in introgression events and consequent impact on host ecology and evolution remain elusive. Three distinct prophages, named C. jejuni integrated elements 1, 2, and 4 (CJIE1, CJIE2, and CJIE4), are described in C. jejuni. Here, we identified two unreported prophages, Campylobacter coli integrated elements 1 and 2 (CCIE1 and CCIE2 prophages), which are C. coli homologues of CJIE1 and CJIE2, respectively. No induction was achieved for both prophages. Conversely, induction assays on CJIE1 and CJIE2 point towards the inducibility of these prophages. CCIE2-, CJIE1-, and CJIE4-like prophages were identified in a Campylobacter spp. population of 840 genomes, and phylogenetic analysis revealed clustering in three major groups: CJIE1-CCIE1, CJIE2-CCIE2, and CJIE4, clearly segregating prophages from C. jejuni and C. coli, but not from human- and nonhuman-derived isolates, corroborating the flowing between animals and humans in the agricultural context. Punctual bacteriophage host-jumps were observed in the context of C. jejuni and C. coli, and although random chance cannot be fully discarded, these observations seem to implicate prophages in evolutionary introgression events that are modulating the hybridization of C. jejuni and C. coli species.F.F.V. is funded by Fundação para a Ciência e a Tecnologia (FCT) through an Assis tant Researcher grant CEECIND/03023/2017, and a project grant (PTDC/BTM-SAL/28978/2017) that supported this work. The work is partially supported by Na-tional funds from FCT, projects UIDB/04138/2020 and UIDP/04138/2020. Campylobacter strains were sequenced under the GenomePT project (POCI-01-0145-FEDER-022184), supported by COMPETE 2020—Operational Programme for Competitiveness and Internationalization (POCI), Lisboa Portugal Regional Operational Programme (Lisboa2020), Algarve Portugal Regional Oper-ational Programme (CRESC Algarve2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF), and by FCT. This work was also supported by Fundos FEDER through the Programa Op eracional Factores de Competitivida-de—COMPETE and by Fundos Nacionais through the FCT within the scope of the project UID/BIM/00009/2019 (Centre for Toxicogenomics and Human Health-ToxOmics).MDPIRepositório Científico do Instituto Nacional de SaúdeTanoeiro, LuísOleastro, MónicaNunes, AlexandraMarques, Andreia T.Duarte, Sílvia VazGomes, João PauloMatos, António Pedro AlvesVítor, Jorge M.B.Vale, Filipa F.2023-01-31T14:27:40Z2022-02-262022-02-26T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/8484engMicroorganisms. 2022 Feb 26;10(3):516. doi: 10.3390/microorganisms10030516.2076-260710.3390/microorganisms10030516info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:42:35Zoai:repositorio.insa.pt:10400.18/8484Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:43:06.159450Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Cryptic Prophages Contribution for Campylobacter jejuni and Campylobacter coli Introgression
title Cryptic Prophages Contribution for Campylobacter jejuni and Campylobacter coli Introgression
spellingShingle Cryptic Prophages Contribution for Campylobacter jejuni and Campylobacter coli Introgression
Tanoeiro, Luís
Campylobacter
Bacteriophage
Host Range
Introgression
Infecções Gastrointestinais
title_short Cryptic Prophages Contribution for Campylobacter jejuni and Campylobacter coli Introgression
title_full Cryptic Prophages Contribution for Campylobacter jejuni and Campylobacter coli Introgression
title_fullStr Cryptic Prophages Contribution for Campylobacter jejuni and Campylobacter coli Introgression
title_full_unstemmed Cryptic Prophages Contribution for Campylobacter jejuni and Campylobacter coli Introgression
title_sort Cryptic Prophages Contribution for Campylobacter jejuni and Campylobacter coli Introgression
author Tanoeiro, Luís
author_facet Tanoeiro, Luís
Oleastro, Mónica
Nunes, Alexandra
Marques, Andreia T.
Duarte, Sílvia Vaz
Gomes, João Paulo
Matos, António Pedro Alves
Vítor, Jorge M.B.
Vale, Filipa F.
author_role author
author2 Oleastro, Mónica
Nunes, Alexandra
Marques, Andreia T.
Duarte, Sílvia Vaz
Gomes, João Paulo
Matos, António Pedro Alves
Vítor, Jorge M.B.
Vale, Filipa F.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Tanoeiro, Luís
Oleastro, Mónica
Nunes, Alexandra
Marques, Andreia T.
Duarte, Sílvia Vaz
Gomes, João Paulo
Matos, António Pedro Alves
Vítor, Jorge M.B.
Vale, Filipa F.
dc.subject.por.fl_str_mv Campylobacter
Bacteriophage
Host Range
Introgression
Infecções Gastrointestinais
topic Campylobacter
Bacteriophage
Host Range
Introgression
Infecções Gastrointestinais
description This article belongs to the Special Issue Bacteriophage Genomics.
publishDate 2022
dc.date.none.fl_str_mv 2022-02-26
2022-02-26T00:00:00Z
2023-01-31T14:27:40Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/8484
url http://hdl.handle.net/10400.18/8484
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Microorganisms. 2022 Feb 26;10(3):516. doi: 10.3390/microorganisms10030516.
2076-2607
10.3390/microorganisms10030516
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dc.publisher.none.fl_str_mv MDPI
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